Pixy Erik Klon Anacının Mikro Çoğaltımında Destek Sistemli Sıvı Besin Ortamının Sürgün Çoğaltma Aşamasında Performansı

Alper Ölmez; Hatice Dumanoğlu; Gülüstan Polat

doi:10.29278/azd.1645026

EN TR

Performance of Liquid Nutrient Medium with Support System in Micropropagation of Pixy Plum Clone Rootstock in Shoot Proliferation Stage

Abstract

Objective: The aim of this study was to investigate the effects of liquid nutrient medium with support system, together with different BAP (6-benzylaminopurine) doses, on shoot survival, multiplication and quality during the shoot proliferation stage in micropropagation of Pixy plum clone rootstock (P. insititia L.). Material and Methods: Micro shoots obtained from subcultures were used as plant material. Modified Murashige and Skoog (MS) nutrient medium containing 0.01 mg L-1 IBA (indole-3-butyric acid) and 0.1 mg L-1 GA3 (gibberellic acid), 0.5 mg L-1 or 1.0 mg L-1 doses of BAP and 30 g L-1 sucrose were added. Semi-solid nutrient medium was solidified with 0.65 g L-1 agar. Filter paper was used as a support system in liquid nutrient medium. Cultures were kept at 25±1 °C and 16 h light (35 µmol m-2 s-1) conditions. At the end of the fourth week, shoot survival percentage and shoot proliferation percentage, shoot number, length, thickness level (1-3) and leaf number in surviving cultures were determined. Results: As an average of BAP doses, shoot survival percentage was found to be significantly higher in liquid medium (%100.0) than in semi-solid medium (%66.7), whereas the proliferation percentage in surviving cultures was statistically significantly higher in semi-solid medium (%91.7) than in liquid medium (%73.6). As an average of all treatments, shoot number and leaf number were determined as 2.8±0.3 and 8.0±0.6 per explant, respectively, and shoot thickness level was 2.0±0.1. The average shoot length was found to be higher in semi-solid medium (10.1±3.1 mm) than in liquid medium (8.0±2.5 mm). Conclusion: The survival percentage of explants in liquid medium was found to be 33.3% higher than in semi-solid medium. In this case, the shoot proliferation percentage calculated according to the survival percentage was 12.4% higher in liquid medium (73.6%) than in semi-solid medium (61.2%). Shoots were generally produced in similar numbers and quality in both media types. As an average of media types, 0.5 mg/L dose of BAP (88.5%) increased the shoot proliferation percentage compared to 1.0 mg/L (72.9%).

Keywords

Prunus insititia , in vitro , 6-benzylaminopurine , semi-solid medium

Pixy Erik Klon Anacının Mikro Çoğaltımında Destek Sistemli Sıvı Besin Ortamının Sürgün Çoğaltma Aşamasında Performansı

Öz

Amaç: Bu çalışmanın amacı, Pixy erik klon anacının (P. insititia L.) mikro çoğaltımında sürgün çoğaltma aşamasında destek sistemli sıvı besin ortamının, farklı BAP (6-benzil amino pürin) dozları ile birlikte sürgün canlılığı, çoğaltımı ve kalitesi üzerine etkilerinin araştırılmasıdır. Materyal ve Yöntem: Bitkisel materyal olarak alt kültürlerden sağlanan mikro sürgünler kullanılmıştır. Modifiye edilmiş Murashige ve Skoog (MS) besin ortamına 0.01 mg L-1 IBA (indol-3-bütirik asit) ve 0.1 mg L-1 GA3 (gibberellik asit) ile birlikte BAP’ın 0.5 mg L-1 veya 1.0 mg L-1 dozları, 30 g L-1 sakaroz ilave edilmiştir. Yarı katı besin ortamı 0.65 g L-1 agar ile katılaştırılmıştır. Sıvı besin ortamında destek sistemi olarak filtre kâğıdı kullanılmıştır. Kültürler 251 oC sıcaklık ve 16 saat aydınlık (35 µmol m-2 s-1) koşullarda tutulmuştur. Dördüncü haftanın sonunda sürgünlerde canlılık oranı, canlı kalan kültürlerde sürgün çoğalma oranı, sürgün sayısı, uzunluğu, kalınlık düzeyi (1-3) ve yaprak sayısı belirlenmiştir. Araştırma Bulguları: Sürgünlerde canlılık oranı BAP dozlarının ortalaması olarak sıvı ortamda (%100.0), yarı katı ortama (%66.7) göre ve canlı kalan kültürlerde çoğalma oranı yarı katı ortamda (%91.7), sıvı ortama (%73.6) göre istatistiksel anlamda önemli düzeyde daha yüksek bulunmuştur. Tüm uygulamaların ortalaması olarak sürgün sayısı 2.8±0.3 ve yaprak sayısı ise 8.0±0.6 adet/eksplant, sürgün kalınlık düzeyi 2.0±0.1 olarak belirlenmiştir. Ortalama sürgün uzunluğu, yarı katı ortamda (10.1±3.1 mm), sıvı ortamdan (8.0±2.5 mm) daha yüksek bulunmuştur. Sonuç: Sıvı ortamda eksplantların canlılık oranı yarı katı ortama göre %33.3 daha yüksek bulunmuştur. Bu durum dikkate alınarak canlılık yüzdesine göre hesaplanan sürgün çoğalma oranı sıvı ortamda (%73.6), yarı katı ortamdan (%61.2) %12.4 daha yüksek gerçekleşmiştir. Her iki ortam tipinde de sürgünler genellikle benzer sayıda ve kalitede üretilmiştir. Ortam tiplerinin ortalaması olarak BAP’ın 0.5 mg L-1 dozu (%88.5), 1.0 mg L-1 dozuna (%72.9) göre sürgün çoğaltım oranını artırmıştır.

Anahtar Kelimeler

Prunus insititia , in vitro , 6-benzil amino pürin , yarı katı ortam

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Details

Primary Language

Turkish

Subjects

Pomology and Treatment

Journal Section

Research Article

Authors

Alper Ölmez
0009-0007-3826-5919
Türkiye

Hatice Dumanoğlu ^*
0000-0002-7099-7630
Türkiye

Gülüstan Polat
0000-0002-8878-4959
Türkiye

Publication Date

June 30, 2025

Submission Date

February 22, 2025

Acceptance Date

May 27, 2025

Published in Issue

Year 2025 Volume: 14 Number: 1

DOI

https://doi.org/10.29278/azd.1645026

IZ

https://izlik.org/JA33DZ88MX

APA

Ölmez, A., Dumanoğlu, H., & Polat, G. (2025). Pixy Erik Klon Anacının Mikro Çoğaltımında Destek Sistemli Sıvı Besin Ortamının Sürgün Çoğaltma Aşamasında Performansı. Akademik Ziraat Dergisi, 14(1), 13-22. https://doi.org/10.29278/azd.1645026

Performance of Liquid Nutrient Medium with Support System in Micropropagation of Pixy Plum Clone Rootstock in Shoot Proliferation Stage

Abstract

Keywords

Pixy Erik Klon Anacının Mikro Çoğaltımında Destek Sistemli Sıvı Besin Ortamının Sürgün Çoğaltma Aşamasında Performansı

Öz

Anahtar Kelimeler

References

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Publication Date

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Acceptance Date

Published in Issue

DOI

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