Fotoperiyot, Potasyum Nitrat ve Giberellik Asidin Lavanta (Lavandula angustifolia) Tohumlarında Dormansinin Kırılması ve Çimlenme Üzerine Etkisi

Öz

Amaç: Bu çalışmanın amacı, fotoperiyot, gibberellik asit (GA3) ve potasyum nitratın (KNO3) lavanta (Lavandula angustifolia) tohumlarında dormansinin kırılması ve çimlenme parametreleri üzerindeki etkilerini araştırmaktır. Materyal ve Yöntem: Lavanta tohumlarının farklı fotoperiyot koşulları (16/8 saat aydınlık/karanlık ve 24 saat karanlık) ile GA3 (0, 125, 250, 375 ve 500 ppm) ve KNO3 (0, 1000, 2000, 3000 ve 4000 ppm) uygulamalarına karşı çimlenme tepkileri kontrollü koşullar altında belirlenmiştir. Çimlenme hızı, çimlenme potansiyeli ve ortalama çimlenme süresi çimlenme parametreleri olarak değerlendirilmiştir Bulgular: Fotoperiyot ve GA3 incelenen tüm çimlenme parametrelerini önemli ölçüde etkilemiştir. 16/8 saat aydınlık/karanlık fotoperiyot koşullarında inkübe edilen lavanta tohumlarında çimlenme hızı %88.4 ve çimlenme potansiyeli %20.0 artarken, ortalama çimlenme süresi karanlıkta inkübe edilen tohumlara kıyasla %16.2 kısalmıştır. En etkili GA3 dozunda (375 ppm) çimlenme hızı ve çimlenme potansiyeli sırasıyla %54.2 ve %31.3 artmış, ortalama çimlenme süresi %29.3 kısalmıştır. KNO3'ün etkisi sadece çimlenme potansiyeli için önemli çıkmış ve 3000 ppm dozunda %12.3 artmıştır. GA3'ün KNO₃ ve ışıkla birlikte uygulanması çimlenme hızı ve potansiyelini daha da artırmıştır. Sonuç: Sonuç olarak, bu bulgular ışığında lavanta tohumlarının çimlenmesini önemli ölçüde uyardığını, 16/8 saat aydınlık/karanlık fotoperiyot koşullarında kombine 375 ppm GA3 ve 3000 ppm KNO₃ uygulamasının lavanta tohumlarında dormansinin kırılması ve çimlenmenin artırılmasında etkili bir yöntem olduğunu göstermektedir.

Anahtar Kelimeler

• GA3
• KNO3
• Çimlenme parametreleri
• Tohum dormansisi

Effect of Photoperiod, Potassium Nitrate and Gibberellic Acid on Dormancy Breaking and Germination of Lavender (Lavandula angustifolia) Seeds

Abstract

Objective: The objective of this study was to investigate the effects of photoperiod, gibberellic acid (GA3) and potassium nitrate (KNO3) on dormancy breaking and germination parameters in lavender (Lavandula angustifolia) seeds. Materials and Methods: The germination responses of lavender seeds to different photoperiod conditions (16/8 h light/dark and 24 h dark), GA3 (0, 125, 250, 375 and 500 ppm) and KNO3 (0, 1000, 2000, 3000 and 4000 ppm) treatments were determined under controlled conditions. Germination speed, germination potential, and mean germination time were evaluated as germination parameters. Results: The photoperiod and GA3 significantly affected all germination parameters studied. Lavender seeds that were incubated under a 16/8 h light/dark photoperiodic condition had an 88.4% increase in germination speed and a 20.0% increase in germination potential, while mean germination time was 16.2% shorter compared to the seeds that were incubated in the dark. The most effective dose of GA3 was 375 ppm, which increased the speed and potential of germination by 54.2% and 31.3%, respectively, while reducing the mean germination time by 29.3%. KNO₃ only had a significant effect on germination potential, which increased by 12.3% at a concentration of 3.000 ppm. The combined application of GA3 with light and KNO₃ further increased the speed and potential of germination. Conclusion: In conclusion, these results suggest that light significantly stimulates lavender seed germination. The combined application of 375 ppm GA3 with 3000 ppm KNO3 in a 16/8 h light/dark photoperiod is an effective treatment for breaking dormancy and increasing germination.

Keywords

• GA3
• germinattion parameters
• KNO3
• seed dormancy

References

1. Alboresi, A., Gestin, C., Leydecker, M. T., Bedu, M., Meyer, C., & Truong, H. N. (2005). Nitrate, a signal relieving seed dormancy in Arabidopsis. Plant Cell Environment, 28(4), 500-512.
2. Aoyama, E., Ono, E., & Furlan, M. (1996). Germination study of lavender seeds. Scientia Agricola, 53(2-3), 267-272.
3. Balouchi, H. R., & Sanavy, A. M. M. (2006). Effect of gibberellic acid, prechilling, sulfiric acid and potassium nitrate on seed germination and dormancy of annual Medics. Pakistan Journal of Biological Sciences, 9(15), 2875-2880.
4. Baskin, J. M., Baskin, C. C., & Xiaojie, L. I. (2000). Taxonomy, anatomy and evolution of physical dormancy in seeds. Plant Species Biology, 15, 139–152.
5. Baskin, J. M., & Baskin, C. C. (2004). A clasification system for seed dormancy. Seed Science Research,14, 1-6.
6. Bell, D. T., King, L. A., & Plummer, J. A. (2009). Ecophysiological effects of light quality and nitrate on seed germination in species from Western Australia. Australian Journal of Ecology, 24, 2–10.
7. Bewley, J. D., & Black, M. (1994). Dormancy and the control of germination. Seeds: Physiology of Development and Germination (2nd ed), Springer Science, NY, 199-271 ps.
8. Caser, M., Demasi, S., Mozzanini, E., Chiavazza, P. M., & Scariot, V. (2022). Germination Performances of 14 Wildflowers Screened for Shaping Urban Landscapes in Mountain Areas. Sustainability, 14(5), 2641.

9. Castro-Camba, R., Sánchez, C., Vidal, N., & Vielba, J. M. (2022). Plant Development and Crop Yield: The Role of Gibberellins. Plants, 11(19), 2650.
10. Cavanagh, H. M. A., & Wilkinson, J. M. (2002). Biological activities of lavender essential oil. Phytotherapy Research, 16(4), 301–308.
11. Chavagnat, A. (1977). Lavender seed dormancy and germination. Acta Horticulturae, 83, 147-154.
12. Crișan, I., Ona, A., Vârban, D., Muntean, L., Vârban, R., Stoie, A., Mihăiescu, T., & Morea, A. (2023). Current Trends for Lavender (Lavandula angustifolia Mill.) Crops and Products with Emphasis on Essential Oil Quality. Plants, 12(2), 357.
13. Dev, R., Dayal, D., & Sureshkumar, M. (2020). Gibberellic acid and potassium nitrate promote seed germination and growth of grey-leaved saucer-berry (Cordia sinensis Lam.) seedlings. International Journal of Fruit Science, 20(2), 937-954.
14. Dissanayake, P., George, D. L., & Gupta, M. L. (2010). Effect of light, gibberellic acid and abscisic acid on germination of guayule (Parthenium argentatum Gray) seed. Industrial Crops and Products, 32(2), 111-117.
15. Du, G., Zhang, H., Yang, Y., Zhao, Y., Tang, K., & Liu, F. (2022). Effects of Gibberellin Pre-Treatment on Seed Germination and Seedling Physiology Characteristics in Industrial Hemp under Drought Stress Condition. Life, 12, 1907.
16. Duermeyer, L., Khodapanahi, E., Yan, D., Krapp, A., Rothstein, S. J., & Nambara, E. (2018). Regulation of seed dormancy and germination by nitrate. Seed Science Research, 28(3), 150-157.
17. Finch-Savage, W. E., & Leubner-Metzger, G. (2006). Seed dormancy and the control of germination. New Phytologist, 171(3), 501–523.
18. Ghavami, T., Kazeminia, M., & Rajati, F. (2022). The effect of lavender on stress in individuals: A systematic review and meta-analysis. Complementary Therapies in Medicine, 68, 102832.
19. Ghaderi-Far, G., Coşgun, Z. L., Değirmenci, Ç. Ü., Tüysüz, İ., Ülgen, Ç., & Tavşanoğlu, Ç. (2021). Light and temperature requirements for germination in the Mediterranean shrub Lavandula stoechas (Lamiaceae). Plant Biology, 23, 992-999.
20. Giray, F. H. (2018). An analysis of world lavender oil markets and lessons for Turkey, Journal of Essential Oil Bearing Plants, 21(6), 1612-1623.
21. ISTA, International Rules for Seed Testing. (2021). Internationl Seed Testing Associatin, Zürichstr. 50, CH-8303 Bassersdorf, Switzerland.
22. Koornneef, M., Bentsink, L., & Hilhorst, H. (2002). Seed dormancy and germination. Current Opinion in Plant Biology, 5, 33–36.
23. Kucerna, B., Cohn, M. A., & Leubner-Metzger, G. (2005). Plant hormone interactions during seed dormancy release and germination. Seed Science Research, 15(4), 281-307.
24. Labbafi, M., Khalaj, H., Delfani, M., & Qavami, N. (2022). Evaluation of different hormonal and temperature treatments on dormancy breaking of Lavender (Lavandula angustifolia) seed. Iranian Journal of Seed Research, 9(1), 163 – 176.
25. Li, X. R., Kang, W. H., Yu, C. Y., & Kim, L. S. (1998). Effects of temperature, light and plant growth regulators on the seed germination of Lavandula angustifolia Mill. Korean Journal of Medicinal Crop Science, 6(4), 258-264.
26. Liopa-Tsakalidi, A., Zakynthinos, G., Varzakas, T., & Xynias, I. N. (2011). Effect of NaCl and GA3 on seed germination and seedling growth of eleven medicinal and aromatic crops. Journal of Medicinal Plants Research, 5(17), 4065-4073.
27. Maher, J., Gerasopoulos, D., & Maloupa, E. (2000). Temperature and light effects on germination of Lavandula stoechas seeds. Acta Horticulturae, 541, 261-264.
28. Nasiri, A. A., Mortazaeinezhad, F., & Taheri, R. (2018). Seed germination of medicinal sage is affected by gibberellic acid, magnetic field and laser irradiation, Electromagnetic Biology and Medicine, 37(1), 50-56.
29. Penfield, S. (2008). Temperature perception and signal transduction in plants. New Phytologist, 179(3), 615–628.
30. Ramzan, A., Hafiz, I. A., Ahmad, T., & Abbasi, N.A. (2010). Effect of priming with potassium nitrate and dehusking on seed germination of gladiolus (Gladiolus alatus). Pakistan Journal of Botany, 42, 247–258.
31. Seo M., Nambara, E., Choi, G., & Yamaguchi. S. (2009). Interaction of light and hormone signals in germinating seeds. Plant Molecular Biology, 69(4), 463-72.
32. Singh, J. M., & Srivastava, I. J. (1990). Seed germination in lavender with acid treatment. Seed Research, 18(1), 86-87.
33. Slimani, C., Sqalli, H., Rais, C., Wafae, S., Lazraq, A., Ghadraoui, L.E., Belmalha, S., & Echchgadda, G. (2020). Improvement of germination rate and in vitro multiplication of Lavandula angustifolia. Journal of Applied Biology & Biotechnology, 8(2), 52-57.
34. Szekely-Varga, Z., Kentelky, E., & Cantor, M. (2021). Effect of gibberellic acid on the seed germination of Lavandula angustifolia Mill. Romanian Journal of Horticulture, 2(2), 169–176.
35. Wu, G. L., Du, G. Z., & Shi, Z. H. (2013). Germination strategies of 20 alpine species with varying seed mass and light availability. Australian Journal of Botany, 61, 404–411.
36. Yamaguchi, S. (2008). Gibberellin metabolism and its regulation. Annual Review of Plant Biology, 59, 225–251. Yamaguchi, S., & Kamiya, Y. (2002). Gibberellins and light-stimulated seed germination. Journal of Plant Growth Regulators, 20, 369-376.
37. Yan, A., & Chen, Z. (2020). The control of seed dormancy and germination by temperature, light and nitrate. Botanical Review, 86, 39–75.
38. Yang, L., Liu, S., & Lin, R. (2020a). The role of light in regulating seed dormancy and germination. Journal of Integrated Plant Biology, 62(9), 1310-1326.
39. Yang, L. E., Peng, D. L., Li, Z. M., Huang, L., Yang, J., & Sun, H. (2020b). Cold stratification, temperature, light, GA3, and KNO3 effects on seed germination of Primula beesiana from Yunnan, China. Plant diversity, 42(3), 168-173.
40. Yücel, E., & Yılmaz, G. (2009). Effects of different alkaline metal salts (NaCl, KNO3), acid concentrations (H2SO4) and growth regulator (GA3) on the germination of Salvia cyanescens Boiss. & Bal. seeds. Gazi University Journal of Science, 22, 123–127.