Researching germination properties of bean (Phaseolus vulgaris L.) under polyethylene glycol osmotic stress and saline conditions
Abstract
Plant growth is negatively affected by environmental factors such as salinity and drought. This research was carried out in the laboratory to determine the germination characteristics of bean cultivar called “Akkuş” under drought and salt stress conditions. The experiment was set up using a completely randomized experimental design with ten replications. For the purpose of the study, germination rate (%), radicle length (cm), plumule length (cm), hypocotyl length (cm), radicle fresh weight (g), plumule fresh weight (g), and hypocotyl fresh weight (g) were calculated. Polyethylene glycol (PEG) 6000 was used to create a dry environment and NaCI was used for a saline environment. Different doses of NaCl (0 (control), 50, 100, 150, and 200 mM) and different osmotic potential levels of PEG 6000 (0 (control), -0.2, -0.4, -0.6, and -0.8 bar) were used in the experiment. The germination rate of bean decreased with increasing salt concentrations. Similarly, an increase in PEG 6000 osmotic potential levels caused a decrease in the parameters. The highest values were obtained in the control groups. As a result of the study, increase in salinity and drought stress cause negative effects on bean germination properties.
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References
- Chen, J., Wu, W., Zheng, Y., Hou, K., Xu, Y., Zai, J., 2010. Drought resistance of Angelica aahurica during seedling stage under polyethylene glycol (PEG-6000) simulated drought stress. China journal of Chinese Materia Medica, 35(2): 149-53.
- Çokkızgın, A., 2012. Salinity stress in common bean (Phaseolus vulgaris L.) seed Germination. Not Bot Horti Agrobo Cluj-Napoca, 40(1): 177–182.
- Çolak, M.S., Avcı, S., Öztürk, A., Yurtseven, E., 2018. Effects of abiotic stress conditions on plants. 1. International GAP Agriculture and Livestock Congress. 25-27 April 2018, Şanlıurfa, Turkey, 171-175.
- Çulha, Ş., Çakırlar, H., 2011. Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları. Afyon Kocatepe University, Journal of Sciences, 11-34.
- Ghanbari, A.A., Mousavi, S.H., Mousapou-Gorji, A., Rao, I., 2013. Effects of water stress on leaves and seeds of bean (Phaseolus vulgaris L.). Turkish Journal of Field Crops, 18(1): 73-77.
- Ghiyasi, M, Tajbakhsh, M., 2013. Osmopriming alleviates drought stress in soybean (Glycine max L.) seeds during germination and early growth stages. Journal of Applied Biological Sciences. 7(1): 27-32.
- Kalefetoğlu Macar, T., Turan, O., Ekmekci, Y., 2009. Effect of water deficit induced by PEG and NaCl on chickpea (Cicer arieitinum L.) cultivar and lines at early seedling stage. Gazi Univercity Journal of Science, 22(1): 5-14.
- Kaya, M.D., Okçu, G., Atak, M., Çıkılı Y., Kolsarıcı Ö., 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). The European Journal of Agronomy. 24: 291–295.
- Kaymakanova, M., 2014. Effect of salinity on germination and seed physiology in bean (Phaseolus vulgaris L.). Biotechnology & Biotechnological Equipment. Special Edition.
- Khan, H., Ayup, A., Pervez, C.M., Bilal, M.A., Shahid, R.M., Ziaf, M.A., 2009. Effect of seed priming with NaCI on salinity tolerance of hot pepper (Capsicum annuum L.) at seedling stage. Soil Environment, 28: 81-87.