Aethionema coridifolium (Brassicaceae) Kümesi Üzerine Moleküler Sistematik Bir Yaklaşım

Year 2020, Volume 7, Issue 3, 61 - 67, 30.12.2020

Kuddisi ERTUĞRUL Tuğba PENSE Meryem BOZKURT Tuna UYSAL

Abstract

Aethionema Aiton Brassicaceae familyasının taksonomik açıdan en zor cinslerinden biridir ve son zamanlarda yayımlanan çok sayıda yeni taksonlarla cins Türkiye’de 33’ü endemik yaklaşık 52 takson ile temsil edilmektedir. Bu çalışmada, Aethionema coridifolium tür kümesi içerisinde değerlendirilen, Ae. coridifolium, Ae. armenum ve Ae. grandiflorum türleri ile bu türlerle yakın ilişkili Ae. huber-morathii ve Ae. karamanicum türleri arasındaki filogenetik ilişkiler ITS gen bölgesi kullanılarak ortaya çıkarıldı. Küme içerisindeki taksonların akrabalık ilişkilerini belirlemek için ITS gen dizileri hizalanarak bir veri matrisi oluşturulmuş ve filogenetik bir ağaç üretilmiştir. Verilere göre Ae. coridifolium kümesi monofiletiktir (BS:%91, PP:1). Oluşturulan ağaca göre Ae. coridifolium kümesinde yer alan taksonların farklı türler olarak değerlendirilmesi kuvvetlice desteklenmektedir.

Keywords

Cruciferae, Filogeni, ITS, Kayagülü, Taksonomi, Türkiye

Abstract

References

• Adıgüzel, N. (2000). Aethionema R. Br. Şu Eserde: Güner, A., Özhatay, N., Ekim, T., Başer, K. H. C. (edlr.). Flora of Turkey and the East Aegean Islands 11: 31-34. Edinburgh University Press. Edinburgh.
• Al-Shehbaz, I.A., Beilstein, M.A. ve Kellogg, E.A. (2006). Systematics and phylogeny of the Brassicaceae: an overview. Plant Systematics and Evolution 259: 89-120.
• Al-Shehbaz, I.A. (2012). A generic and tribal synopsis of the Brassicaceae (Cruciferae). Taxon 61: 931-954.
• Bailey, C.D., Koch, M.A., Mayer, M., Mummenhoff, K., O’Kane, S.L. Jr., Warwick, S.I., Windham, M.D. ve Al-Shehbaz, I.A. (2006). Towards a global phylogeny of the Brassicaceae. Molecular Biology and Evolution 23: 2142-2160.
• Bartolucci, F. et al. (2018). An updated checklist of the vascular flora native to Italy. Plant Biosystems 152: 179-303.
• Batur Ö.S. (2014). Aethionema W.T.Aiton (Brassicaceae) Cinsinin Moleküler Filogenisi (Yüksek lisans tezi). Hacettepe Üniversitesi, Fen Bilimleri Enstitüsü, Ankara.
• Czerepanov, S.K. (1995). Vascular plants of Russia and adjacent states (the former USSR), s.s. 516. Cambridge University Press, Cambridge.
• Cullings, K.W. (1992). Design and testing of a plant-specific PCR primer for ecological and evolutionary studies, Molecular Ecology 1: 233-240.
• Davis, P.H. (1988). Aethionema R. Br. Şu eserde: Davis, P.H. (ed.). Flora of Turkey and the East Aegean Islands 10: 35-39. Edinburgh Univ. Press, Edinburgh.
• Doyle, J.J. ve Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, Botanical Society of America 19: 11-15.
• Ertuğrul, K. (2012). Aethionema Aiton. Şu eserde: Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (edlr.). Türkiye Bitkileri Listesi (Damarlı Bitkiler), 246-248. Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul.
• German, D.A., Friesen, N., Neuffer, B., Al-Shehbaz, I.A. ve Hurka, H. (2009). Contribution to ITS phylogeny of the Brassicaceae, with special reference to some Asian Taxa. Plant Systematics and Evolution 283 (1): 33-56.
• Greuter, W., Burdet, H. M., Long, G. (1986). Med-Checklist 3. Conservatoire et Jardin Botaniques, Genève.
• Hall, J.C., Systsma, K.J. ve Iltis, H.H. (2002). Phylogeny of Capparaceae and Brassicaceae based on chloroplast sequence data. American Journal of Botany 89: 1826-1842.
• Hall, T.A. (1999). Bio Edit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acid Symposium 41: 95-98.
• Hedge, I.C. (1965). Aethionema R. Br. Şu eserde: Davis, P.H. (ed.) Flora of Turkey and the East Aegean Islands 1: 314-330. Edinburgh Univ. Press, Edinburgh
• Hedge, I.C. (1968). Aethionema R. Br. & Moriera Boiss. Şu Eserde: Hedge, I.C. & Rechinger, K.H. (edlr.). Flora Iranica 57: 100-110, 354. Academische Drucku. Verlagsanstalt, Graz.
• Hedge, I.C. ve Lamond, J.L. (1980). Brassicaceae. Şu eserde: Townsend, C.C. & Guest, E. (edlr.). Flora of Iraq 4: 915-922. Ministry of Agriculture, Baghdad.
• Kandemir, A., Aytaç, Z. ve Fişne, A. Y. (2017). Aethionema erzincanum (Brassicaceae), a new species from Turkey. Annales Botanici Fennici 54 (1-3): 1-5.
• Karabacak, O., Öztürk, M. ve Duran, A. (2013). Aethionema anatolica (Brassicaceae), a new species from South Anatolia, Turkey. Annales Botanici Fennici 50: 183-186.
• Khosravi, A.R, Mohsenzadeh, S. ve Mummenhoff, K. (2009). Phylogenetic relationships of Old World Brassicaceae from Iran based on nuclear ribosomal DNA sequences. Biochemical Systematics and Ecology 37: 106-115.
• Koch, M. (2003). Molecular phylogenetics, evolution and population biology in Brassicaceae. Şu eserde: Sharma, A.K. ve Sharma, A. (edlr.) Plant Genome: Biodiversity and Evolution 1: Phanerograms, 1-35. Science Publishers Inc., Enfield.
• Kumar, S., Stecher, G., Li, M., Knyaz, C. ve Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35:1547-1549.
• Meyer, F.K. (1973). Conspectus der "Thlaspi"-Arten Europas, Afrikas und Vorderasiens. Feddes Repert 84: 449-470.
• Moazzeni, H., Al-Shehbaz I.A., German D.A. Assadi M., Müller J., Joharchi M.R. ve Memariani F. (2018). A taxonomic revision of the genus Aethionema s.l. (Brassicaceae) in Iran. Phytotaxa 356 (4): 241-266.
• Mohammadin, S., Peterse, K., van de Kerke, S.J., Chatrou, L.W., Dönmez, A.A., Mummenhoff, K., Pires, J.C., Edger, P.P., Al-Shehbaz, I.A.ve Schranz, M.E. (2017). Anatolian origins and diversification of Aethionema, the sister lineage of the core Brassicaceae. American Journal of Botany 104 (7): 1042-1054.
• Montemurro, M. (1993). Aethionema. Şu Eserde: Castroviejo, S., Aedo, C., Gómez-Campo, C., Laínz, M., Montserrat, P., Morales, R., Muñoz Garmendia, F., Nieto Feliner, G., Rico, E., Talavera, S. & Villar, L. (edlr.). Flora Iberica 4: 264-269. Real Jardín, Botánico, Madrid.
• Özüdoğru, B., Özgişi, K., Tarıkahya-Hacıoğlu, B., Ocak, A., Mummenhoff, K., Al-Shehbaz, I. (2019). Phylogeny of the genus Noccaea Moench (Brassicaceae) and a critical review of its generic circumscription. Annals of the Missouri Botanical Garden 104(3): 339-354.
• Pavlova, D. (2007). A new species of Aethionema (Brassicaceae) from the Bulgarian flora. Botanical Journal of the Linnean Society 155:533-540.
• Pınar, N.M., Adıgüzel, N. ve Geven, F. (2007). Seed coat macrosculpturing in some Turkish Aethionema R. Br. (Brassicaceae), Pakistan Journal of Botany 39: 1025-1036.
• Pignatti, S. (1982). Flora d’Italia 1:790 Edagricole.
• Prantl, K. (1891). Eunomia, Die natürlichen Pflanzenfamilien, (edlr.: Engler, A., & Prantl K.), Engel-mann, Leipzig, 3(2).
• Ronquist, F., Teslenko, M., Mark, P.V.D., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A., ve Huelsenbeck J.P. (2012). MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. Syst. Biol. 61(3): 539-542.
• Soltis, P.S. ve Doyle, J.J. (1998). Molecular systematics of plants II: DNA sequencing, Kluwer Academic Pub, 2.
• Soltis, D.E., Collier, T.G., Edgerton, M.L. (1991). The Heuchera group (Saxifragaceae): Evidence for chloroplast transfer and paraphyly. American Journal of Botany 78: 1091-1112.
• Swofford, D.L. (1999). PAUP 4.0: Phylogenetic Analysis Using Parsimony (And Other Methods). Sinauer Associates Inc, Sunderland, MA.
• Strid A., Tan K. (edlr.) (2002). Flora Hellenica 2: xvi+511. Gantner Verlag, Ruggell.
• The Bulgarian flora online. (2006-2017). https://bgflora.net/index_en.html (Erişim tarihi: 15.11.2020).
• Warwick, S.I. ve Sauder, C. (2005). Phylogeny of tribe Brassiceae (Brassicaceae) based on chloroplast restriction site polymorphisms and nuclear ribosomal internal transcribed spacer and chloroplast trnL intron sequences. Canadian Journal of Botany 83: 467-483.
• Warwick, S.I., Sauder, C.A., Al-Shehbaz, I.A. ve Jacquemoud, F. (2007). Phylogenetic relationships in the tribes Anchonieae, Chorisporeae, Euclidieae and Hesperideae (Brassicaceae) based on nuclear ribosomal ITS DNA sequences. Annals of the Missouri Botanical Garden 94: 56-78.
• Warwick, S.I., Sauder, C.A. ve Al-Shehbaz, I.A. (2008). Phylogenetic relationships in the tribe Alysseae (Brassicaceae) based on nuclear ribosomal ITS DNA sequences. Canadian Journal of Botany 86: 315-336.
• White, T.J., Bruns, T., Lee, S., Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. ve White, T.J. (edlr.) PCR Protocols: A Guide to Methods and Applications s.s. 315–322. Academic Press, New York.
• Yıldırımlı, S. ve Kılıç, Ö. (2016). New infrageneric taxa and species of Aethionema W.T. Aiton (Brassicaceae) and their current key from Turkey. Ot Sistematik Botanik Dergisi 23 (1-2): 1-6.
• Yıldırımlı, S. ve Kılıç, Ö. (2018). A new species of Aethionema (Brassicaceae) A. adiyamanense from Turkey. Ot Sistematik Botanik Dergisi 25 (1): 25-30.
• Yıldırımlı, S. ve Kılıç, Ö. (2019). A new Aethionema (Brassicaceae), A. sancakense p.p. and a new description of A. adıyamanense from Turkey. Ot Sistematik Botanik Dergisi 26 (2): 49-58.