Sebzelerde Hücre Süspansiyon Kültürleri

Öz

Bitki biyokimyası ve moleküler biyoloji ile ilgili temel çalışmalarda yararlanılan önemli bir araç olan bitki hücre kültüründe çeşitli yöntemler mevcut olup bunlar arasında, farklılaşmış kültürler (tüm bitki ve organ kültürleri; sürgün, kök ve adventif kök kültürleri) veya farklılaşmamış kültürler (kallus-hücre süspansiyon kültürleri, protoplast kültürü) yer almaktadır. Bitki hücre süspansiyon kültürleri, tüm bir bitki organizmasının yapısal karmaşıklığını göz ardı ederek, çeşitli olayların araştırılması amacıyla pratik bir araç olarak sıklıkla kullanılmaktadır. Süspansiyon tekniği ile kültüre alınmış hücreler, in vitro hücre popülasyonunun homojenliği, materyalin geniş ölçekte mevcudiyeti, hücre gelişiminin hızlı temposu ve koşulların güçlü bir şekilde tekrarlanabilir olması sayesinde karmaşık fizyolojik süreçlerin hücresel ve moleküler düzeyde araştırılmasına olanak sağlar. Bu değerlendirmede başlangıç materyalleri olan eksplantlardan başlayarak bitki hücre süspansiyon kültürlerinin nasıl başlatılacağı ve sürdürüleceği açıklanmakta ve sebzelerde uygulanmış hücre süspansiyon kültürü çalışmalarından örnekler sunulmaktadır.

Anahtar Kelimeler

• In vitro
• doku kültürü
• hücre süspansiyon kültürü

Cell Suspension Cultures in Vegetables

Abstract

There are various methods in plant cell culture, which is an important tool used in basic studies on plant biochemistry and molecular biology, and these include differentiated cultures (whole plant and organ cultures; shoot, root and adventitious root cultures) or undifferentiated cultures (callus-cell suspension cultures, protoplast culture). Plant cell suspension cultures are frequently used as a practical tool for the investigation of various phenomena, ignoring the structural complexity of an entire plant organism. Cells cultured with the suspension technique enable the investigation of complex physiological processes at the cellular and molecular level, thanks to the homogeneity of the in vitro cell population, the large-scale availability of the material, the rapid pace of cell development and the strong reproducibility of the conditions. In this research, it is explained how to initiate and maintain plant cell suspension cultures, starting from explants, which are the starting materials, and examples of cell suspension culture studies applied to vegetables are presented.

Keywords

• In vitro
• tissue culture
• cell suspension culture

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