Zambak (Lilium spp.) Soğan Pullarının, in vitro Koşullarda Köklendirilmesi Üzerine, Farklı Besin Ortamlarının Etkilerinin Belirlenmesi

Öz

Lilium spp. kesme çiçek yada tıbbi amaçla farklı alanlarda değerlendirilebilen soğanlı bir süs bitkisidir. Zambaklar, vejetatif olarak soğan pulları ayırma, doku kültürü gibi yöntemlerle veya generatif olarak tohumla çoğaltılabilmektedir. Çiçeklenmenin uzun sürmesi ve heterozigot yapıdan kaynaklı genetik açılmalar sebebiyle, bu türün vejetatif çoğaltma teknikleriyle başarılı olarak çoğaltılabilmesi, bitkinin sürdürülebilirliği ve muhafazası açısından oldukça önem taşımaktadır. Ülkemizde süs bitkileri ticaretinde, soğanlı bitkilerin ithalatının oldukça yüksek olduğu göz önünde bulundurulduğunda, soğan üretiminde dışa bağımlığı azaltmak amacıyla yapılan çalışmaların arttırılması, yeni uygulamaların ve mevcut araştırmaların ilerletilmesi gerekmektedir. Bu bağlamda, zambak soğan pullarının in vitro koşullarda köklendirilmesi amacıyla gerçekleştirilen çalışmada, farklı oranlarda Murashige ve Skoog (MS) besin ortamı (1-1/2) ve değişik oranlarda oksin kaynakları (0-0,5-1 mg.l⁻¹ 3-İndol Bütirik Asit (IBA) ve 1-Naftalenil Asetik Asit (NAA)) denenmiştir. Çalışmanın sonuçlarına göre, yaprak uzunluğu (48,62 mm), köklenme oranı (%100), kök uzunluğu (40,32 mm), yaprak sayısı (5,25 adet/bitki) ve pul sayısı (9,17 adet/bitki) açısından en iyi sonuçlar, ½ MS+1 mg.l⁻¹ IBA içerikli ortamda tespit edilirken, bitki yaş ağırlığı (5,46 g) bakımından 0.5 mg.l⁻¹ NAA, soğan çapı (9,65 mm) için ise 1 mg.l⁻¹ NAA takviye edilmiş ortamdan en yüksek sonuçlar belirlenmiştir. Tüm parametreler birlikte değerlendirildiğinde, ½ MS+1 mg.l⁻¹ IBA içerikli ortamın, zambak soğan pullarının in vitro çoğaltımında etkili olduğu sonucuna varılmıştır.

Anahtar Kelimeler

• Lilium
• Soğan Pulu
• In Vitro
• Köklendirme.

Evaluating the Impact of Various Nutrient Media on the in vitro Rooting Efficiency of Lily (Lilium spp.) Bulb Scales

Abstract

Lilium spp. is a bulbous ornamental plant that can be utilized in different areas for cut flowers or medicinal purposes. Lilies can be propagated vegetatively by methods such as bulb scale separation, tissue culture or generatively by seed. Due to the long flowering period and genetic openings caused by heterozygous structure, the successful propagation of this species with clonal propagation techniques is significant for the sustainability and conservation of the plant. Considering that the import of bulbous plants is quite high in the ornamental plants trade in our country, it is necessary to increase the studies carried out so as to reduce the foreign dependence on onion production, to advance new applications and existing researches. In this context, MS nutrient medium (1-1/2) at different ratios and auxin sources (0-0,5-1 mg.l⁻¹ IBA and NAA) at different ratios were tried in the study carried out for rooting of lily bulbs under in vitro conditions. Based on the findings of the study, the best results in relation to leaf length (48,62 mm), rooting rate (100%), root length (40,32 mm), number of leaves (5.25 pcs/plant) and number of scales (9.17 pcs/plant) were obtained from ½ MS+1 mg.l⁻¹ IBA, while for plant fresh weight (5,46 g), 0.5 mg.l⁻¹ NAA and for bulb diameter (9,65 mm), the highest results were obtained from 1 mg.l⁻¹ NAA supplemented medium. In the comprehensive evaluation of all parameters, it was concluded that the composition that consists of ½ MS+1 mg.l⁻¹ IBA was effective in micropropagation of lily bulb scales.

Keywords

• Lilium
• Bulb Scale
• In Vitro
• Rooting.

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