Patlıcanda Toprak Kökenli Fungal Etmenlere Karşı Çoklu Dayanıklılık Geliştirmek İçin Gen Piramitleme Çalışmaları

Abstract

Dünya genelinde açıkta ve örtüaltında geniş ekiliş alanı olan patlıcanın yetiştiriciliğini kısıtlayan en önemli faktörler toprak kökenli patojenlerdir. Bunlardan özellikle Fusarium oxysporum f.sp. melongenae ve Verticillium dahliae’nin neden olduğu solgunluk hastalıkları, verim kayıplarına yol açmaktadır. Hastalık etmenleri ile mücadelede çevre dostu ve en kalıcı yöntem, dayanıklı çeşit kullanmaktır. Ancak bu hastalıklara dayanıklı kültür formunda ticari çeşit geliştirme çalışmalarından henüz yeterli düzeyde çıktılara ulaşılamamıştır. Batı Akdeniz Tarımsal Araştırma Enstitüsü’nde 2015-2021 yılları arasında yürütülen araştırmalar kapsamında, heterozis ve geriye melezleme metodu kullanılarak bitki ve meyve özellikleri bakımından arzu edilen özelliklere sahip, aynı zamanda Fusarium ve Verticillium solgunluk etmenlerine dayanıklılık sağlayan genleri de bulunduran ıslah hatları geliştirilmiştir. Böylece birden fazla hastalığa dayanıklılık gösteren nitelikli ticari çeşit ıslahı için gen piramitlemesi tekniğinden yararlanma esasında çalışmalar yapılmaktadır. Çoklu dayanıklılık gösteren ıslah hatlarının çeşit geliştirme programlarında kullanılması ile birlikte kısa sürede ticari çeşitler geliştirmenin yolu açılmıştır.

Keywords

• Fusarium
• Verticillium
• dayanıklılık
• verim
• meyve kalitesi
• bitki yapısal özellikleri

Gene Pyramiding Studies to Develop Multiple Resistances Against Soilborne Fungi in Eggplant

Abstract

Soil-borne pathogens are the most important factors limiting the cultivation of eggplant, which has a wide cultivation area in the open and under cover all over the world. Especially wilt diseases caused by Fusarium oxysporum f.sp. melongenae and Verticillium dahliae, leads to yield losses. The most environmentally friendly and most permanent method of combating disease agents is to use resistant varieties. However, sufficient outputs have not been reached yet from commercial cultivar development studies in the form of culture resistant to these diseases. Within the scope of the research carried out at the Batı Akdeniz Agricultural Research Institute between 2015-2021, breeding lines with the desired characteristics in terms of plant and fruit characteristics, as well as genes that provide resistance to Fusarium and Verticillium wilt fungi, were developed using the heterosis and backcross method. Thus, studies are carried out on the basis of utilizing the gene pyramiding technique for the breeding of qualified commercial varieties that are resistant to more than one disease. The use of breeding lines showing multiple resistance in cultivar development programs paved the way for developing commercial cultivars in a short time.

Keywords

• Fusarium
• Verticillium
• yield
• resistance
• fruit quality
• plant architecture features

References

1. Alam, I., M. Salimullah, 2021. Genetic engineering of eggplant (Solanum melongena L.):progress, controversy and potential. Horticulturae 7(4):78.
2. Aldrich, C., C.A. Cullis, 1993. CTAB DNA extraction from plant tissues. Plant Molecular Biology Reporter 11(2):128-141.
3. Altınok, H.H., C. Can, 2010. Characterization of Fusarium oxysporum f.sp. melongenae isolates from eggplant in Turkey by pathogenicity, VCG and RAPD analysis. Phytoparasitica 38(2):149-157.
4. Altınok, H.H., H.F. Boyacı, V. Topçu, 2012. Antalya, Mersin ve Samsun illeri örtü altı patlıcan üretim alanlarında Fusarium ve Verticillium solgunluklarının yaygınlığı ve izolatların virülensliklerinin coğrafi dağılımı. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 43(2):107-115.
5. Barchi, L., L. Toppino, D. Valentino, L. Bassolino, E. Portis, S. Lanteri, G.L. Rotino, 2018. QTL analysis reveals new eggplant loci involved in resistance to fungal wilts. Euphytica 214(2):1-15.
6. Boyacı, H.F. 2007. Patlıcanlarda Fusarium solgunluğuna dayanıklılık kaynakları ve dayanıklılığın kalıtımı (Doktora Tezi). Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Adana 96s.
7. Boyacı, H.F., V. Topçu, A. Ünlü, E. Gümrükçü, H. İkten, 2013. Patlıcanda Fusarium oxysporum f.sp. melongenae’ya dayanıklı ve Verticillium dahliae’ye tolerant hatların geliştirilmesi (Proje Sonuç Raporu) (2009-2013, DPT-2004K120170 ve özel sektör tohumculuk firmaları tarafından desteklenmiştir.).
8. Boyaci, H.F., J. Prohens, A. Unlu, E. Gumrukcu, M. Oten, M. Plazas, 2020. Association of heterotic groups with morphological relationships and general combining ability in eggplant. Agriculture 10(6):203.

9. Boyacı, H.F. 2021. Patlıcan ıslahı. A. Eren, (Ed.):Yazlık sebze ıslahı (domates, biber, patlıcan, hıyar, kavun). Nobel Akademik Yayıncılık s:155-188. ISBN 978-625-439-257-3, 292s.
10. Cappelli, C., V.M. Stravato, G.L. Rotino, R. Buonaurio, 1995. Source of resistance among Solanum spp. to an Italian isolate of Fusarium oxysporum f.sp. melongenae. 9. Eucarpia Meeting on Genetics and Breeding of Capsicum & Eggplant. Budapest (Hungary), 21-25 August, 221-224.
11. Collonnier, C., I. Fock, V. Kashyap, G.L. Rotino, M.C. Daunay, Y. Lian, I.K. Mariska, M.V. Rajam, A. Servaes, G. Ducreux, D. Sihachakr, 2001. Applications of biotechnology in eggplant. Plant Cell, Tissue and Organ Culture 65(2):91-107.
12. Daunay, M.C., J. Salinier, X. Aubriot, 2019. Crossability and diversity of eggplants and their wild relatives. In The Eggplant Genome. Springer, Cham. pp:135-191.
13. Deketelaere, S., L. Tyvaert, S.C. França, M. Höfte, 2017. Desirable traits of a good biocontrol agent against Verticillium wilt. Frontiers in microbiology 8:1186.
14. Dervis, S., H. Yetisir, H. Yıldırım, F.M. Tok, S. Kurt, F. Karaca, 2009. Genetic and pathogenic characterization of Verticillium dahliae isolates from eggplant in Turkey. Phytoparasitica 37:467-476.
15. FAO, 2020-a. Food and Agriculture Organization of the United Nations (FAO) Bitkisel üretim verileri. (https://www.fao.org/faostat/en/#data/qcl Erişim:17 Eylül 2022).
16. FAO, 2020-b. Food and Agriculture Organization of the United Nations (FAO) Yıllık nüfus verileri. (https://www.fao.org/faostat/en/#data/oa; Erişim:17 Eylül 2022).
17. Fukuoka, S., S.I. Yamamoto, R. Mizobuchi, U. Yamanouchi, K. Ono, N. Kitazawa, N. Yasuda, Y. Fujita, T.T.T. Nguyen, S. Koizu, K. Sugimoto, T. Matsumuto, M. Yano, 2014. Multiple functional polymorphisms in a single disease resistance gene in rice enhance durable resistance to blast. Scientific Reports 4(1):1-7.
18. Fukuoka, S., N. Saka, Y. Mizukami, H. Koga, U. Yamanouchi, Y. Yoshioka, N. Hayashi, K. Ebana, R. Mizobuchi, M. Yano, 2015. Gene pyramiding enhances durable blast disease resistance in rice. Scientific reports 5(1):1-7.
19. Gaion, L.A., L.T. Braz, R.F. Carvalho, 2018. Grafting in vegetable crops:A great technique for agriculture. International Journal of Vegetable Science 24(1):85-102.
20. Garibaldi, A., A. Minuto, M.L. Gullino, 2005. Verticillium wilt incited by Verticillium dahliae in eggplant grafted on Solanum torvum in Italy. Plant Disease 89:777.
21. Gebeloğlu, N., Ş.Ş. Ellialtıoğlu, 2022. Patlıcan ıslahı. K., Abak, A. Balkaya, Ş.Ş. Ellialtıoğlu ve E. Düzyaman (Ed):Sebze Islahı Cilt-3 Solanaceae (Patlıcangiller). Gece Kitaplığı s:319-412. ISBN:978-625-430-116-2.
22. Gramazio, P., J. Prohens, S. Vilanova, 2021. Genomic resources in the eggplant wild genepool. In the wild solanums, Genomes Springer, Cham. pp:189-200.
23. Gümrükcü, E., A. Ünlü, H.F. Boyacı, V. Topçu, N. Karatekin, 2014. Verticillium solgunluk hastalığına (Verticillium dahliae Kleb.) karşı patlıcan hatlarının reaksiyonlarının belirlenmesi. Türkiye 5. Bitki Koruma Kongresi, 3-5 Şubat 2014, Antalya.
24. Hoagland, D.R., D.I. Arnon, 1950. The water-culture method for growing plants without Soil. California College Agricultural Experiment Station Cire. Berkeley, Circular 347.
25. Kashyap, V., S.V. Kumar, C. Collonnier, F. Fusari, R. Haicour, G.L. Rotino, D. Sihachakr, M.V. Rajam, 2003. Biotechnology of eggplant. Scientia Horticulturae 97(1):1-25.
26. Kefalogianni, I., D. Gkizi, E. Pappa, L. Dulaj, S.E. Tjamos and I. Chatzipavlidis, 2017. Combined use of biocontrol agents and zeolite as a management strategy against Fusarium and Verticillium wilt. BioControl 62(2):139-150.
27. Kim, S.K., W.K. Kim, W.E. Park, S.S. Hong, 2000. Occurrence of eggplant wilt caused by Verticillium dahliae. Plant Pathology Journal 16 (3):156-161.
28. Miller, A.S., C.R. Rowe, M.R. Riedel, 1996. Fusarium and Verticillium wilts of tomato, potato, pepper and eggplant. The Ohio State University Extension Plant Pathology, HGY-3122-96. 2021 Cofey Road. Columbus, OH 432101087.
29. Miyatake, K., T. Saito, S. Negoro, H. Yamaguchi, T. Nunome, A. Ohyama, H. Fukuoka, 2016. Detailed mapping of a resistance locus against Fusarium wilt in cultivated eggplant (Solanum melongena). Theoretical and Applied Genetics 129(2):357-367.
30. Mochizuki, H., Y. Sakata, K. Yamakawa, T. Nishio, S. Komochi, T. Nariakawa, S. Monma, 1997. Eggplant parental line 1’ and eggplant breeding line resistant to Fusarium wilt. Bulletin of the National Research Institute of Vegetables, Ornamental Plants and Tea. Series A:Vegetables and Ornamental Plants 12:85-90.
31. Monma, S., I. Sato, H. Matsunaga, 1996. Evaluation of resistance to bacterial, Fusarium and Verticillium in eggplant and eggplant-related species collected in Ghana. Capsicum and Eggplant Newsletter 15:71-72.
32. Mutlu, N., F.H. Boyaci, M. Göçmen, K. Abak, 2008. Development of SRAP, SRAPRGA, RAPD and SCAR markers linked with a Fusarium wilt resistance gene in eggplant. Theoretical and Applied Genetics 117:1303-1312.
33. Panth, M., S.C. Hassler, F. Baysal-Gurel, 2020. Methods for management of soilborne diseases in crop production. Agriculture 10(1):16.
34. Pegg, G.F., 1981. Biochemistry and physiology of pathogenesis. In:E.M. Mace, A.A. Bell, C.H. Beckman (Eds):Fungal wilt disease of plants. Academic Press Inc. London, ISBN:0-12-464450-3.
35. Pitrat, M., G. Risser, C. Epinat, C. Ferrière, M. Ricard, C. Olivier, A. RuYnato, H. Lecoq, D. Blancard, F. Bertrand, A. Nicot, A. Glandard, P.M. Molot, P. Mas, 1991. Techniques d’inoculation artiwcielle du melon avec divérents agents pathogènes pour la sélection de variétés résistantes. Informal technical Bulletin edited by INRA, Station d’Amélioration des Plantes maraîchères and Station de Pathologie végétale, Montfavet, France, 8p.
36. Prohens, J., B.D. Whitaker, M. Plazas, S. Vilanova, M. Hurtado, M. Blasco, P. Gramazio, J.R. Stommel, 2013. Genetic diversity in morphological characters and phenolic acids content resulting from an interspecific cross between eggplant, Solanum melongena, and its wild ancestor (S.incanum). Annals of Applied Biology 162(2):242-257.
37. Rajam, M.V., S.V. Kumar, 2007. Eggplant. In Transgenic Crops IV. Springer, Berlin, Heidelberg. pp:201-219.
38. Rakha, M., J. Prohens, D. Taher, T.H. Wu, S.O. Solberg, 2021. Eggplant (Solanum melongena, S.aethiopicum and S.macrocarpon) breeding. In Advances in Plant Breeding Strategies:Vegetable Crops. Springer, Cham. pp:163-203.
39. Rotino, G.L., G. Mennella, F. Fusari, G. Vitelli, M.G. Tacconi, A. D’Alessandro, N. Acciarri, 2001. Towards introgression of resistance to Fusarium oxysporum f.sp. melongenae form Solanum integrifolium into eggplant. 11. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Antalya, Turkey, pp:303-307.
40. Rotino, G.L., T. Sala, L. Toppino, 2014. Eggplant. In Alien Gene Transfer in Crop Plants, 2:381-409. Springer, New York, NY.
41. Sakata, Y., S. Monma, T. Narikawa, S. Komochi, 1996. Evaluation of resistance to bacterial wilt and Verticillium wilt in eggplants (Solanum melongena L.) collected in Malaysia. Journal of the Japanese Society for Horticultural Science, 65(1):81-88.
42. Sarkar, M., I. Panigrahi, S. Praneetha, R. Muthuselvi, 2022. Current status of brinjal (Solanum melongena L.) Diseases and their management. In Diseases of Horticultural Crops. Apple Academic Press. pp:1-32.
43. Sekara A., S. Cebula, E. Kunicki, 2007. Cultivated eggplants-origin, breeding objectives and genetic resources, a review. Folia Horticulturae Ann. 19(1):97-114.
44. Swarup, V., 1995. Genetics resources and breeding of aubergine (Solanum melongena L.), Acta Horticulturae 412:71-79.
45. Tassone, M.R., P. Bagnaresi, F. Desiderio, L. Bassolino, L. Barchi, F.E. Florio, F. Sunseri, T.M. Sirangelo, G.L. Rotino, L. Toppino, 2022. A genomic BSAseq approach for the characterization of QTLs underlying resistance to Fusarium oxysporum in eggplant. Cells 11(16):2548.
46. Thies, J.A., 2021. Grafting for managing vegetable crop pests. Pest Management Science 77(11):4825-4835.
47. Toppino, L., G. Valè, G.L. Rotino, 2008. Inheritance of Fusarium wilt resistance introgressed from Solanum aethiopicum Gilo and Aculeatum groups into cultivated eggplant (S.melongena) and development of associated PCR-based markers. Molecular Breeding 22(2):237-250.
48. Toppino, L., J. Prohens, G.L. Rotino, M. Plazas, M. Parisi, C. Carrizo García, P. Tripodi, 2021. Pepper and eggplant genetic resources. In The Wild Solanums Genomes. Springer, Cham. pp:119-154.
49. Van Steekelenburg, N.A.M., 1976. Fusarium wilt of eggplant in the Netherlands. Netherlands Journal of Plant Pathology 82(5):191-192.
50. Varvara, R.A., K. Szabo, D.C. Vodnar, 2021. 3D food printing:Principles of obtaining digitally-designed nourishment. Nutrients 13(10):3617.
51. Wei, Q., L. Du, W. Wang, T. Hu, H. Hu, J. Wang, K. David, C. Bao, 2019. Comparative transcriptome analysis in eggplant reveals selection trends during eggplant domestication. International Journal of Genomics, Article ID 7924383.
52. Yücel, S., 1989. Domates Fusarium solgunluğuna (Fusarium oxysporum Schlecht f.sp. Iycopersici (Sacc.) Synd. and Hans) karşı biyolojik kontrolde antagonistlerin ve toprak solarizasyon uygulamasının karşılıklı etkileşimlerinden yararlanma olanakları üzerinde araştırmalar. Adana Zirai mücadele Araştırma Enstitüsü Müdürlüğü, Ankara, Yayın No:64.
53. Yücel, S., Elekçioğlu, İ.H., Uludağ, A., Can, C., Söğüt, M., Özarslandan, A., Aksoy, E. 2002. The first year result of methyl bromide alternatives in strawberry, pepper and eggplant in the Eastern Mediterranean Part of Turkey. Annual International Research Conference on Methyl Bromide Alternatives and Emissions Reductions. 5-9 November, San Diego, California 92108.
54. Yücel, S., A. Özarslandan, C. Can, 2015. Örtü altı sebze ve çilek yetiştiriciliğinde toprak dezenfeksiyonu uygulamaları. Harran Tarım ve Gıda Bilimleri Dergisi 19(3):144-150.