Bitki Gelişimini Teşvik Eden Bazı Bakterilerin White Dynasty Lale (Tulipa sp. L.) Çeşidinin Gelişimine Etkisi

Year 2025, Volume: 54 Issue: Özel Sayı 1, 300 - 304, 25.03.2025

Rukiye Gezer , Utku Şanver , Hatice Özaktan

https://doi.org/10.53471/bahce.1557498

Abstract

Bu çalışmada White dynasty lale çeşidine, özgün ve yararlı bakteri ırklarının gelişim ve çiçeklenme üzerine etkileri araştırılmıştır. Ege Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Bakteriyoloji Laboratuvarı kültür koleksiyonuna ait endofitik karakterli olan 117 adet aday yararlı bakteri izolatı in vitro olarak siderofor aktivitesi, IAA üretimi, ACC deamilaz aktivitesi ve Fosfataz Aktivitesi testleri yapılmıştır. In vitro PGPR testlerinin sonucuna üzerinden yapılan tartılı derecelendirme metodu ile en başarılı dört yararlı bakteri bitkiler üzerinde uygulanmak üzere seçilmiştir. Bakterilerin OD 600 = 0.1 (~1×10⁸ cfu/ml) yoğunluğundaki süspansiyonları 5 ml olarak içirme şeklinde bitki köklerine uygulanmıştır. Çalışma, tesadüf parselleri deneme desenine göre 3 tekerrürlü ve her tekerrürde 3 adet bitki olacak şekilde laboratuvar koşullarında kurulmuştur. Lalelerin yaprak sayısı, yaprak uzunluğu, yaprak genişliği, bitki boyu, çiçek boyu, çiçek genişliği ve sap kalınlığı incelenmiştir. Çalışmanın sonucunda en yüksek yaprak sayısı, en uzun bitki boyu, en yüksek yaprak uzunluğu, en fazla yaprak genişliği 213 numaralı bakteri uygulamasından; en yüksek çiçek boyunun ve en geniş çiçek çapı kontrol uygulamasından elde edilmiştir. Yürütülen çalışmada lale yetiştiriciliği için incelenen parametreler bakımından 213 no.lu özgün izolatına ait bakteri uygulamasının diğer uygulamalara nazaran daha fazla olumlu etki oluşturduğu gözlemlenmiştir.

Keywords

Çiçeklenme, PGPB, Tulipa. sp. L.

Effect of Some Plant Growth Promoting Bacteria on the Development of White Dynasty Tulip (Tulipa L.) Variety

Abstract

In this study, the effects of specific and beneficial bacterial strains on the development and flowering of White dynasty tulip variety were investigated. In vitro siderophore activity, IAA production, ACC deamylase activity and phosphatase activity tests were performed on 117 candidate beneficial bacterial isolates with endophytic character belonging to the culture collection of Ege University, Faculty of Agriculture, Department of Plant Protection, Department Bacteriology Laboratory. The most successful four beneficial bacteria were selected to be applied on plants by the weighted ranking method based on the results of in vitro PGPB tests. OD 600 = 0.1 (~1×10⁸ cfu/ml) suspensions of bacteria were applied to the plant roots as 5 ml by drinking. The study was established under laboratory conditions according to the randomized plot design with 3 replications and 3 plants in each replication. The number of leaves, leaf length, leaf width, plant height, flower length, flower width and stem thickness of tulips were examined. As a result of the study, the highest leaf number, the longest plant height, the highest leaf length, and the largest leaf width were obtained from the 213 numbered bacteria application; the highest flower length and the largest flower diameter were obtained from the control application. In the study conducted, it was observed that the bacteria application of the original isolate numbered 213 had a more positive effect than the other applications in terms of the parameters examined for tulip cultivation.

Keywords

Flowering, PGPB, Tulipa. sp. L.

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