Bazı Bahçe Nanesi (Mentha spicata L.) Genotiplerinin Biyolojik Aktivite Özellikleri

Year 2025, Volume: 54 Issue: 2, 93 - 101, 27.11.2025

Yalçın Kaya , Gül Kuşaksız (tarımcılar) , Özer Yılmaz

https://doi.org/10.53471/bahce.1747304

Abstract

Bu çalışmada, 32 bahçe nanesi (Mentha spicata) genotipinin toplam fenolik ve flavonoid miktarı ile antioksidan aktiviteleri araştırılmıştır. Toplam fenolik madde miktarları 2,74 ile 8,34 mg GAE.100 mg⁻¹ arasında değişirken, toplam flavonoid madde miktarları 4,35 ile 10,36 mgQE.100 mg⁻¹ olarak belirlenmiştir. Genotiplerin 2,2-difenil-1-pikrilhidrazil (DPPH) serbest radikal giderme aktivitesi IC₅₀ değerleri 0,97 ile 2,27 mg.mL⁻¹ aralığında tespit edilmiştir. Bakır iyonu indirgeme antioksidan kapasitesi (CUPRAC) ise 18,66 ile 47,85 mgTE.100 mg⁻¹ arasında değişiklik göstermiştir. Toplam fenolik ve flavonoid madde miktarları ile antioksidan aktiviteler arasında anlamlı korelasyonlar bulunmuştur. Elde edilen bulgular, bazı Mentha spicata genotiplerinin fenolik ve flavonoid içeriklerinin etkili bir antioksidan potansiyel sağladığını ortaya koymakta olup, bu genotiplerin gıda, ilaç ve kozmetik sektörlerinde doğal antioksidan kaynağı olarak değerlendirilebileceğini göstermektedir.

Keywords

Nane , Toplam Fenolik Madde , Toplam Flavanoid Madde , DPPH , CUPRAC

Supporting Institution

Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü

Project Number

TAGEM/TBAD/B/20/A7/P6/2072

Biological Activity Properties of Some Spearmint (Mentha spicata L.) Genotypes

Abstract

In this study, the total phenolic and flavonoid contents as well as the antioxidant activities of 32 spearmint (Mentha spicata) genotypes were investigated. The total phenolic contents ranged from 2.74 to 8.34 mgGAE.100 mg⁻¹, while the total flavonoid contents were determined to be between 4.35 and 10.36 mgQE.100 mg⁻¹. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of the genotypes showed IC₅₀ values ranging from 0.97 to 2.27 mg.mL⁻¹. The cupric ion reducing antioxidant capacity (CUPRAC) values varied between 18.66 and 47.85 mgTE.100 mg⁻¹. Significant correlations were found between total phenolic and flavonoid contents and antioxidant activities. The findings indicate that certain some Mentha spicata genotypes possess effective antioxidant potential due to their high phenolic and flavonoid contents, suggesting that these genotypes could be utilized as natural antioxidant sources in the food, pharmaceutical and cosmetic industries.

Keywords

Mentha spicata , Total phenolic content , Total flavonoid content , DPPH , CUPRAC

Project Number

TAGEM/TBAD/B/20/A7/P6/2072

References

• Inoue, M., Hayashi, S., Craker, L.E., 2019. Role of medicinal and aromatic plants: Past, present, and future. In: Perveen, S., Al-Taweel, A. (Eds.), Pharmacognosy-Medicinal Plants. Intech Open, London, UK, pp:1-13.
• World Health Organization, 2022. WHO establishes the Global Centre for Traditional Medicine in India. https://www.who.int/news/ item/25-03-2022-who-establishes-the-global-centre-for-traditional-medicine-in-india (Erişim Tarihi: 1 Temmuz 2025).
• Bakır, Ö., 2020. Sekonder metabolitler ve rolleri. Uluslararası Anadolu Ziraat Mühendisliği Bilimleri Dergisi, 2(4), 39-45.
• Khan, A., Kanwal, F., Ullah, S., Fahad, M., Tariq, L., Altaf, M.T., Zhang, G., 2025. Plant secondary metabolites-central regulators against abiotic and biotic stresses. Metabolites, 15(4), 276.
• Kochhar, S.L., Gujral, S.K., 2020. Secondary Plant Metabolites. In: Plant Physiology: Theory and Applications. Cambridge University Press, Cambridge, pp:590-610.
• Edo, G.I., Nwachukwu, S.C., Ali, A.B., Yousif, E., Jikah, A.N., Zainulabdeen, K., Essaghah, A.E.A., 2024. A review on the composition, extraction and applications of phenolic compounds. Ecological Frontiers.
• Naseem, I., Khan, M.A., Habib, U., Rana, R.M., Qasim, M., Alwahibi, M.S., Iqbal, R., 2025. Morphological profiling and DNA barcoding revealed genetic diversity and phylogeny of Mentha species cultivated in Pakistan. Genetic Resources and Crop Evolution, 72(3), 2977-2995.
• Mamadalieva, N.Z., Akramov, D.K., Ovidi, E., Tiezzi, A., Nahar, L., Azimova, S.S., Sarker, S.D., 2017. Aromatic medicinal plants of the Lamiaceae family from Uzbekistan: Ethnopharmacology, essential oils composition, and biological activities. Medicines, 4(1), 8.
• Hudz, N., Kobylinska, L., Pokajewicz, K., Horčinová Sedláčková, V., Fedin, R., Voloshyn, M., Myskiv, I., Brindza, J., Wieczorek, P.P., Lipok, J., 2023. Mentha piperita: essential oil and extracts, their biological activities, and perspectives on the development of new medicinal and cosmetic products. Molecules 28(21), 7444.
• Rita, I., Pereira, C., Barros, L., Santos-Buelga, C., Ferreira, I.C., 2016. Mentha spicata L. infusions as sources of antioxidant phenolic compounds: Emerging reserve lots with special harvest requirements. Food & Function, 7(10), 4188-4192.
• Shaikh, S., Yaacob, H.B., Rahim, Z.H.A., 2014. Prospective role in treatment of major illnesses and potential benefits as a safe insecticide and natural food preservative of mint (Mentha spp.): A review. Asian Journal of Biomedicine and Pharmaceutical Sciences, 4, 1-12.
• Kuşaksız, G., Kaynak, G., 2002. Karadeniz Bölgesi Mentha L. (Labiatae) taksonları üzerine morfolojik bir araştırma. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 5(3).
• Mahendran, G., Verma, S.K., Rahman, L.U., 2021. The traditional uses, phytochemistry and pharmacology of spearmint (Mentha spicata L.): A review. Journal of Ethnopharmacology, 278, 114266.
• El-Haoud, H., Boufellous, M., Berrani, A., Tazougart, H., Bengueddour, R., 2018. Screening phytochimique D’une plante medicinale: Mentha spicata L. American Journal of Innovative Research & Applied Sciences, 7(4), 226-233.
• Cemeroğlu, B., 2010. Gıda Analizleri (2. Baskı). Gıda Teknolojisi Yayınları Derneği, Yayın No:39, Ankara.
• Thaipong, K., Boonprakob, U., Crosby, K., Cisneros-Zevallos, L., Byrne, D.H., 2006. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis, 19(6-7), 669-675.
• Lafka, T.I., Sinanoglou, V., Lazos, E.S., 2007. On the extraction and antioxidant activity of phenolic compounds from winery wastes. Food Chemistry, 104(3), 1206-1214.
• Apak, R., Güçlü, K., Özyürek, M., Karademir, S.E., 2004. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC Method. Journal of Agricultural and Food Chemistry, 52(26), 7970-7981.
• Karadeniz, F., Burdurlu, H.S., Koca, N., Soyer, Y., 2005. Antioxidant activity of selected fruits and vegetables grown in Turkey. Turkish Journal of Agriculture and Forestry, 29(4), 297-303.
• Anwar, F., Abbas, A., Mehmood, T., Gilani, A.H., Rehman, N.U., 2019. Mentha: a genus rich in vital nutra-pharmaceuticals - a review. Phytotherapy Research, 33(10), 2548-2570.
• Kanatt, S.R., Chander, R., Sharma, A., 2007. Antioxidant potential of mint (Mentha spicata L.) in radiation-processed lamb meat. Food Chemistry, 100, 451-458.
• Cirlini, M., Mena, P., Tassotti, M., Herrlinger, K. A., Nieman, K.M., Dall’Asta, C., & Del Rio, D. 2016. Phenolic and volatile composition of a dry spearmint (Mentha spicata L.) extract. Molecules, 21(8), 1007.
• Ćavar Zeljković, S., Šišková, J., Komzáková, K., De Diego, N., Kaffková, K., Tarkowski, P., 2021. Phenolic compounds and biological activity of selected Mentha species. Plants, 10(3), 550.
• Capecka, E., Mareczek, A., Leja, M., 2005. Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chemistry, 93(2), 223-226.
• Scherer, R., Lemos, M.F., Martinelli, G.C., Martins, J.D.L., da Silva, A.G., 2013. Antioxidant and antibacterial activities and composition of Brazilian spearmint (Mentha spicata L.). Industrial Crops and Products, 50, 408-413.
• Suresh, S.M., Suresh, S.N., Sagadevan, P., Kumar, S.R., 2014. In vitro antioxidant activity of methanolic leaf extract of Mentha arvensis. JPBR, 2(2), 115-204.
• Mirzaei, A., Rezanejad, M.T., Mirzaei, N., 2015. Phytochemical and antiradical properties of alcoholic and aqueous extracts of Red capsicum and Mentha piperita. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(3), 174-179.
• Abdel-Hady, H., El-Wakil, E.A., Abdel-Gawad, M., 2018. GC-MS analysis, antioxidant and cytotoxic activities of Mentha spicata. European Journal of Medicinal Plants, 26(1), 1-12.
• Nickavar, B. Alinaghi, A. Kamalinejad, M., 2008. Evaluation of the antioxidant properties of five Mentha species. Iran. J. Pharm. Res. 7, 203-209.
• Yolcu, M.S., Öksüz, A., Güler, E., Turan, F., 2024. Tıbbi nane (Mentha piperita L.) bitkisinin fide gelişim döneminde uygulanan farklı BAP, IBA ve IAA hormon dozlarının büyüme ve biyokimyasal parametreler üzerine etkileri. Journal of Agricultural Biotechnology, 5(2), 63-70.
• Meriç, Z.İ., Nath, E.Ö., Doğan, A., Bitiş, L., 2024. Antioxidant, anti-tyrosinase activities and characterization of phenolic compounds for some plants from the Marmara Region, Türkiye. Journal of Research in Pharmacy, 28(2), 396-408.
• George, J., Edwards, D., Pun, S., Williams, D., 2022. Evaluation of antioxidant capacity (ABTS and CUPRAC) and total phenolic content (Folin-Ciocalteu) assays of selected fruit, vegetables, and spices. International Journal of Food Science, (1), 2581470.
• Kapp, K., 2015. Polyphenolic and essential oil composition of Mentha and their antimicrobial effect. Dissertation. Faculty of Pharmacy of the University of Helsinki, 90p.
• Shafiee, Z., Samani, B.H., Rostami, S., Lorigooini, Z., Jmashidi-kia, F., Taki, K., 2024. Enhancing the quality attributes of mint medicinal plant (Mentha spicata L.) through simultaneous combined ozone and infrared drying. Journal of Food Process Engineering, 47(1), e14529.
• İçli, N., 2022. Taşköprü’de yetiştirilen tıbbi nanenin antioksidan özelliklerinin belirlenmesi. Kastamonu Üniversitesi Sağlık Bilimleri Fakültesi Dergisi, 1(1), 1-10.
• Asyhar, R., Minarni, M., Arista, R.A., Nurcholis, W., 2023. Total phenolic and flavonoid contents and their antioxidant capacity of Curcuma xanthorrhiza accessions from Jambi. Biodiversitas Journal of Biological Diversity, 24(9).
• Adıgüzelli, G., Osman, E., Varol, T., Şimşek, S., Kandemir, A., 2024. Erzincan ili-Ergan dağının farklı rakımlarında yayılış gösteren bazı taksonların antioksidan kapasiteleri. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 7(5), 2315-2332.