Cinnamomum verum Uçucu Yağının Pseudomonas aeruginosa ve Acinetobacter baumannii Üzerindeki Anti-biyofilm ve Anti-quarum Sensing Aktivitesi

Year 2023, , 169 - 176, 15.03.2023

Esin Kıray

https://doi.org/10.53424/balikesirsbd.1071396

Abstract

ÖZ
Amaç: Günümüzde mevcut kullanımdaki antimikrobiyaller üzerinde dirençli bakterilerinin sayısının artması halk sağlığı açısından büyük tehlike oluşturmaktadır. Çoklu ilaca dirençli patojenlerin ortaya çıkması, alternatif tedavi stratejilerinin araştırılmasını zorunlu hale getirmiştir. Bitki uçucu yağları hastalıkların tedavisinde toksik olmayan, uygun maliyetli ve erişilebilir olması nedeni ile alternatif antimikrobiyaller olarak değerlendirilmektedir. Bu çalışmada, Cinnamomun verum yaprağı esansiyel yağının (EY) Pseudomonas aeruginosa ve Acinetobacter baumannii üzerindeki antibakteriyel ve anti-biyofilm etkisi ile anti-quarum sensing (QS) özelliklerini değerlendirmek amaçlamıştır. Gereç ve Yöntem: Çalışmada C. verum EY’nın P. aeruginosa ve A. baumannii üzerindeki Minimum İnhibitör Konsantrasyon (MIC) ve Minimum Bakterisidal Konsantrasyon (MBC) ları belirlenmiştir. Minimum inhibitör konsantrasyonlarının (MIC'ler) belirlenmesinde mikrodilüsyon yöntemi kullanılmıştır. C. verum EY’nin kimyasal içeriği, Gaz Kromatografisi-Kütle Spektrometrisi (GC-MS) kullanılarak belirlenmiştir. Bulgular: C. verum EY test edilen suşlara karşı 0,5 ve 1,0 mg/mL oranlarında MIC ve MBC göstermiştir. C. verum EY’nin 2 mg/mL konsantrasyonda yüksek oranda anti-QS aktivite gösterdiği belirlenmiştir. GC-MS sonucuna göre C. verum yağında en bol bulunan bileşik sinnamaldehit olarak bulunmuştur. Sonuç: C. verum EY’nin bakteriyel biyofilmlerin neden olduğu enfeksiyonlara karşı potansiyel kullanım alanı oluşturabilecek etkinliğe sahiptir. C. verum EY’lerin anti-QS aktivitesi üzerine yapılan çalışmalar sınırlı olup, bu alanda daha çok patojen mikroorganizma üzerindeki anti-QS aktivitesi araştırılmalıdır.

Keywords

Cinnamomun verum, Uçucu yağ, Anti-biyofilm

Supporting Institution

Kırşehir Ahi Evran Üniversitesi Bilimsel Araştırma Projeleri

Project Number

Proje No: PYO-FEN.4001.16.012

Thanks

Bu araştırma Kırşehir Ahi Evran Üniversitesi Bilimsel Araştırma Projeleri Proje No: PYO-FEN.4001.16.012 tarafından desteklenmiştir.

Anti-biofilm and Anti-quarum Sensing Activity of Cinnamomum verum Essential Oil on Pseudomonas aeruginosa and Acinetobacter baumannii

Abstract

Objective: The increase in the number of bacteria resistant to antimicrobials in current use poses a great danger to public health. The emergence of multi-drug-resistant pathogens on antimicrobials in current use has made it necessary to search for alternative treatment strategies. Plant essential oils are considered as alternative antimicrobials in the treatment of diseases because they are non-toxic, cost-effective and accessible. This study aimed to evaluate the antibacterial and antibiofilm effect and anti-quarum sensing (QS) properties of Cinnamomun verum leaf essential oil on Pseudomonas aeruginosa and Acinetobacter baumannii. Materials and Methods: In the study, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of EO on P. aeruginosa and A. baumannii were determined. The microdilution method was used to determine the minimum inhibitory concentrations (MICs). The chemical content of C. verum EO was determined using Gas Chromatography-Mass Spectrometry (GC-MS). Results: C. verum EO showed MIC and MBC at 0.5 and 1.0 mg/mL versus strains tested. It was determined that C. verum EO showed high anti-QS activity at a concentration of 2 mg/mL. According to GC-MS results, cinnamaldehyde was found to be the most abundant compound in C. verum EO. Conclusion: C. verum EO has potential to be used against infections caused by bacterial biofilms. Studies on the anti-QS activity of C. verum EOs are limited, and the anti-QS activity on pathogenic microorganisms should be investigated in this area.

Keywords

Cinnamon verum, Essential oil, Anti-biofilm, Anti-quarum detection

Project Number

Proje No: PYO-FEN.4001.16.012

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