The Enzyme Histochemistry of Eimeria-Infected Lambs

Year 2024, , 434 - 440, 28.08.2024

Mehmet Özüiçli , İhsan Kısadere , Mehmet Faruk Aydın

https://doi.org/10.53424/balikesirsbd.1469416

Abstract

Objective: We aimed to investigate the effects of Eimeria infection on ANAE-positive lymphocyte percentages of lambs in the present study. Materials and Methods: For this purpose, fecal samples were taken from 200 animals into sterile containers on day 0. Eimeria positivity was detected in lambs according to the Fulleborn saturated saline method. Besides, oocyst load was detected by using the Mc. Master method in lambs. Forty of 200 lambs (n=40) aged approximately 60-75 days from a farm where Merino sheep were raised were included in this study. Experimental groups were established as Eimeria-positive male lambs (n =10), Eimeria-negative male lambs (n =10), Eimeria-positive female lambs (n =10), Eimeria-negative female lambs (n =10). Blood samples were collected to determine ANAE-positive lymphocyte percentages in lambs. Results: Results of the present study shown that Eimeria positivity was detected in 10 male and 10 female lambs according to the Fulleborn saturated saline method. According to the Mc. Master method, oocyst load was determined between 10.000-15.000 in male and female positive lambs. Eimeria-positive male and female lambs had the greatest ANAE-positive lymphocyte percentages in our study (p < 0.05). Conversely, the lowest ANAE-positive lymphocyte percentages were found in Eimeria-negative female lambs, which were not statistically different from male lambs in the present study. It was the first report according to the detection of ANAE-positive lymphocyte percentages in Eimeria-infected lambs. Conclusion: In conclusion, ANAE-positive lymphocyte percentages can be affected by Eimeria positivity and oocyst load (between 10.000-15.000) both in male and also female lambs.

Keywords

Eimeria, ANAE positivity, Lymphocyte, Lambs.

Project Number

Yayınımızın herhangi bir proje kapsamından türetilmemiştir.

References

• Abdel-Saeed, H., & Salem, N. Y. (2019). Clinical, hematologic, sero-biochemical and IgE response in lambs with diarrhea caused by Eimeria. International Journal of Veterinary Science, 8(1), 10-13.
• Abdulmageed, Z. M., & Mohamed, A. E. (2022). Effect of Eimeria infection on hematology and biochemistry of sheep at Sohag Governorate, Egypt. Journal of Applied Veterinary Sciences, 7(2), 66-72. https://doi.org/10.21608/javs.2022.123302.1130
• Alcala-Canto, Y., Figueroa-Castillo, J. A., Ibarra-Velarde, F., Vera-Montenegro, Y., & Cervantes-Valencia, M. E. (2020). First database of the spatial distribution of Eimeria species of cattle, sheep and goats in Mexico. Parasitology Research, 119, 1057-1074. https://doi.org/10.1007/s00436-019-06548-8
• Al-dujaily, A. H., & Al-mialy, A. J. (2017). Clinical and hemato-biochemical studies in Awassi lambs infected with coccidiosis. Kufa Journal for Veterinary Medical Sciences, 8(1), 1-7. https://doi.org/10.36326/kjvs/2017/v8i14322
• Aydin, M. F., Celik, I., & Sur, E. (2012). Investigation of α-naphthyl acetate esterase and acid phosphatase in the peripheral blood leukocytes of greyhounds. Biotechnic and Histochemistry, 87, 265–272. https://doi.org/10.3109/10520295.2011.639717
• Aziz, S. J., & Mahmoud, O. I. (2022). Measurement of hematological and biochemical parameters in sheep infected with intestinal protozoa and helminthes. Tikrit Journal of Pure Science, 26(1), 8-11. http://dx.doi.org/10.25130/tjps.26.2021.002
• Baberi, N. A. S., Razmi, G., Karimi, I., Nourani, H., & Azizi, H. (2021). Evaluation of parasitological, hematological and biochemical parameters in experimental infection of lamb with Eimeria ahsata. Iranian Journal of Veterinary Clinical Sciences, 14(1), 79-87.
• Carneiro, P. G., Sasse, J. P., Silva, A. C. D. S., Seixas, M., Paschoal, A. T. P., Minutti, A. F., … & Garcia, J. L. (2022). Prevalence and risk factors of Eimeria spp. natural infection in sheep from Northern Paraná, Brazil. Brasilian Journal of Veterinary Parasitology, 31(1), e017421. https://doi.org/10.1590/S1984-29612022004
• Cazarotto, C. J., Boito, J. P., Gebert, R. R., Reis, J. H., Machado, G., Bottari, N. B., & Da Silva, A. S. (2018). Metaphylactic effect of minerals on immunological and antioxidant responses, weight gain and minimization of coccidiosis of newborn lambs. Research in Veterinary Science, 121, 46-52. https://doi.org/10.1016/j.rvsc.2018.09.003
• Donmez, H. H., & Sur, E. (2008). Hematology and enzyme histochemistry of the peripheral blood leucocytes in rock partridges (Alectoris graeca). Poultry Science, 87, 56-60. https://doi.org/10.3382/ps.2007-00328
• Donmez, N., Donmez, H. H., Kisadere, I., Kadiralieva, N. (2016). Some hematological values and alpha-naphthyl acetate esterase (ANAE)-positive lymphocyte ratios in Jaydara sheep. Journal of Advanced Veterinary and Animal Research, 3(1), 8-12. http://doi.org/10.5455/javar.2016.c124
• Donmez, H. H., Donmez, N., Kısadere, I., & Undag, I. (2019). Protective effect of quercetin on some hematological parameters in rats exposed to cadmium. Biotechnic and Histochemistry, 94, 381-386. https://doi.org/10.1080/10520295.2019.1574027
• Elitok, B. (2020). Epidemiology of coccidiosis and effects of the infection on some clinical and hematological examination findings in calves. Journal of Istanbul Veterinary Sciences, 4(1), 21-25. http://doi.org/10.30704/http-www-jivs-net.671228
• Filipenko, O. V., & Soroka, N. M. (2023). Hematological and biochemical indicators of lambs’ blood after the use of chemotherapeutic agents for spontaneous eimeriosis. Regulatory Mechanisms in Biosystems, 14(4), 595-600. https://doi.org/10.15421/022386
• Ghanem, M. M., & Abd el-raof, Y. M. (2005). Clinical and haemato-biochemical studies on lamb coccidiosis and changes following amprolium and sulphadimthoxine therapy. Benha Veterinary Medical Journal, 16(2), 286-99.
• Gokbulut, C., Ozuicli, M., Aksit, D., Aksoz, E., Korkut, O., Yalcinkaya, M., & Cirak, V. Y. (2016). Comparative plasma and milk dispositions, faecal excretion and efficacy of per os ivermectin and pour‐on eprinomectin in horses. Journal of Veterinary Pharmacology and Therapeutics, 39(6), 584-591. https://doi.org/10.1111/jvp.12308
• Hermosilla, C., Bürger, H. J., & Zahner, H. (1999). T cell responses in calves to a primary Eimeria bovis infection: phenotypical and functional changes. Veterinary Parasitology, 84(1-2), 49-64. https://doi.org/10.1016/S0304-4017(99)00075-8
• Hughes, H. P., Thomas, K. R., & Speer, C. A. (1988). Antigen-specific lymphocyte transformation induced by oocyst antigens of Eimeria bovis. Infection and Immunity, 56(6), 1518-1525. https://doi.org/10.1128/iai.56.6.1518-1525.1988
• Hughes, H. P., Speer, C. A., Kyle, J. E., & Dubey, J. P. (1987). Activation of murine macrophages and a bovine monocyte cell line by bovine lymphokines to kill the intracellular pathogens Eimeria bovis and Toxoplasma gondii. Infection and Immunity, 55, 784–791. https://doi.org/10.1128/iai.55.3.784-791.1987
• Keeton, S.T.N., & Navarre, C. B. (2017). Coccidiosis in large and small ruminants. Veterinary Clinics: Food Animal Practice, 34 (1): 201-208. https://doi.org/10.1016/j.cvfa.2017.10.009
• Kisadere, İ., Kadyralieva, N., Sur, E., Dönmez, N., & Oruç, E. (2016). Some physiological, hematological values and ANAE-positive lymphocyte rations of domestic donkeys (Equus asinus) in Kyrgyzstan. Journal of Kafkas Üniversitesi Veterinerlik Fakültesi Dergisi, 23, 165-168. https://doi.org/10.9775/kvfd.2016.15784
• Maiti, N. K., Saini S. S., & Sharma S. N. (1990). Histochemical studies on chicken peripheral blood lymphocytes. Veterinary Research Communication, 14, 207-210.
• Martins, N. S., Motta, S. P. D., Santos, C. C., Moreira, A. S., Farias, N. A., & Ruas, J. L. (2020a). Eimeria spp. infection in lambs from southern Brazil. Pesquisa Veterinária Brasileira, 40, 871-874. https://doi.org/0.1590/1678-5150-PVB-6745
• Martins, N. S., Motta, S. P., Santos, C. C., Moreira, A. S., & Farias, N. A. R. (2020b). Eimeriosis in cattle and sheep: an invisible enemy. Brazilian Journal of Development, 6 (4), 19421-19434. https://doi.org/10.34117/bjdv6n4-201
• Matos, L., Muñoz, M. C., Molina, J. M., Rodríguez, F., Pérez, D., López, A. M., & Ruiz, A. (2018). Age-related immune response to experimental infection with Eimeria ninakohlyakimovae in goat kids. Research in Veterinary Science, 118, 155-163. https://doi.org/10.1016/j.rvsc.2018.02.004
• Olmos, L. H., Caro, L. C., Avellaneda-Cáceres, A., Medina, D. M., Sandoval, V., Aguirre, D. H., & Micheloud, J. F. (2020). First record of clinical coccidiosis (Eimeria ovinoidalis) in adult sheep from northwestern Argentina. Veterinary Parasitology: Regional Studies and Reports, 21, 100429. https://doi.org/10.1016/j.vprsr.2020.100429
• Pérez, D., Muñoz, M. C., Molina, J. M., Muñoz-Caro, T., Silva, L. M. R., Taubert, A., ... & Ruiz, A. (2016). Eimeria ninakohlyakimovae induces NADPH oxidase-dependent monocyte extracellular trap formation and upregulates IL-12 and TNF-α, IL-6 and CCL2 gene transcription. Veterinary Parasitology, 227, 143-150. https://doi.org/10.1016/j.vetpar.2016.07.028
• Połozowski, A., Zawadzki, W., & Nowak, M. (2006). Comparison of two fecal flotation techniques for diagnostic of internal parasites infections in swine and dogs. Electronic Journal of Polish Agricultural Universities, 9, 39.
• Rakhshandehroo, E., Nazifi, S., Razavi, S. M., Ghane, M., & Alavi, A. M. (2013). Caprine coccidiosis: the effects of induced infection on certain blood parameters. Veterinarski Arhiv, 83(6), 623-631.
• Ruiz, A., Muñoz, M. C., Molina, J. M., Hermosilla, C., Andrada, M., Lara, P., ... & Taubert, A. (2014). Immunization with Eimeria ninakohlyakimovae-live attenuated oocysts protect goat kids from clinical coccidiosis. Veterinary Parasitology, 199(1-2), 8-17. https://doi.org/10.1016/j.vetpar.2013.09.032
• Taubert, A., Hermosilla, C., Sühwold, A., & Zahner, H. (2008). Antigen-induced cytokine production in lymphocytes of Eimeria bovis primary and challenge infected calves. Veterinary Immunology and Immunopathology, 126(3-4), 309-320. https://doi.org/10.1016/j.vetimm.2008.09.003
• Trejo-Huitrón, G., Bautista-Gómez, L. G., Martínez-Castañeda, J. S., Romero-Núñez, C., Trejo-Castro, L., & Espinosa-Ayala, E. (2020). Morphological characterization and first molecular identification of the eleven Eimeria species that infect sheep from Mexico. Parasitology Research, 119, 115-122. https://doi.org/10.1007/s00436-019-06477-6 .