OMEGA–3 YAĞ ASİTLERİNİN BÖBREK ANTİOKSİDAN SAVUNMA SİSTEMİ ÜZERİNDEKİ ETKİSİ: DENEYSEL BİR ÇALIŞMA

Year 2012, Volume: 1 Issue: 2, 70 - 74, 30.08.2012

Burak Gülcen Ömür Karaca Murat Abdulgani Kuş Dilara Kaman Murat Ögetürk İlter Kuş

Abstract

AMAÇ: Bu çalışmada böbrek dokusu üzerine omega – 3 yağ asitlerinin koruyucu etkisinin biyokimyasal düzeyde araştırılması amaçlandı.

GEREÇ VE YÖNTEM: Toplam 16 adet Wistar – Albino cinsi erişkin erkek sıçan iki gruba ayrıldı ve incelendi. Grup I’deki sıçanlar kontrol grubu olarak kullanıldı ve intragastrik gavaj yöntemi ile serum fizyolojik verildi. Grup II’deki sıçanlara ise, 400 mg/kg dozundaki omega – 3 yağ asidi intragastrik gavaj yöntemi ile günlük olarak verildi. Altı haftalık deney süresinden sonra sıçanlar dekapitasyon yöntemi ile öldürüldü. Hayvanlardan alınan böbrek doku örneklerinde malondialdehit (MDA) düzeyi ve superoksit dizmutaz (SOD), glutatyon peroksidaz (GSH - Px) enzim aktiviteleri spektrofotometri yöntemi ile tayin edildi.

BULGULAR: Çalışmamızda omega – 3 yağ asiti verilen sıçanların böbrek SOD ve GSH – Px aktiviteleri istatistiksel açıdan anlamlı bir şekilde artarken MDA düzeylerinin ise kontrol grubuna göre anlamlı bir düşüşü tespit edildi.

SONUÇ: Omega – 3 yağ asitlerinin sıçan böbrek dokusunda antioksidan savunma sistemini güçlendirdiği, oksidatif hasarı önlediği ve böbrek dokusu üzerinde koruyucu etki gösterdiği belirlendi.

Keywords

Omega – 3 yağ asiti, böbrek

EFFECTS OF OMEGA - 3 FATTY ACIDS ON KIDNEY ANTIOXIDANT DEFENSE SYSTEM: AN EXPERIMENTAL STUDY

Abstract

OBJECTİVE: In this research, protective effect of omega – 3 fatty acids on kidney tissue is biochemically investigated.

MATERİAL AND METHOD: For his purpose, 16 adult Wistar – Albino rats are divided into two groups and investigated. Rats in Group I are used as control group and they are only intragastric gavaged with normal saline. However, rats in Group II are intragastric gavaged daily with a dose of 400 mg/kg fatty acid. All rats are killed by using decapitation method at the end of six weeks experiment period. Kidney tissues collected from experimental subjects are spectrophotometric investigated for malondialdehyde level and superoxide dismutase (SOD), glutathione peroxidase (GSH – Px) enzyme activities.

RESULTS: It has been observed that SOD and GSH – Px activities in kidney are statistically increased in rats treated with omega – 3 fatty acid. Along with this, there had been statistical decrease in their MDA levels compared to the results coming from control group subjects.

CONCLUSİON: It is concluded that omega – 3 fatty acids prevent the oxidative damage in rat kidney tissue. These fatty acids have positive antioxidant effect, thus protect kidney tissue.

Keywords

Omega – 3 fatty acid, kidney

References

• 1. Nordoy A. Is there a rational use for n – 3 fatty asids (fish oils) in clinical medicine ?. Drugs 1991; 42: 331-342.
• 2. Bouurre JM, Bonneil M, Chaudiere J, Clement M, Dumont O, Durant G. Structural and functional importance of dietary polyunsaturated fatty acids in the nervous system. Adv Exp Med Biol 1992; 18 (1): 211-229.
• 3. Storlien LH, Higgins JA, Thomas TCI. Diet composition and insülin action in animal models. Br J Nutr 2000; 83: 85-90.
• 4. Mantzioris E, Cleland LG, Gibson RA. Biochemical effects of a diet containing foods enriched with n-3 fatty acids. Am J Clin Nutr 2000; 72: 42-48.
• 5. Zararsız İ, Kuş İ, Akpolat N, Songur A, Ögetürk M, Sarsılmaz M. Protective effects of omega - 3 essential fatty acids agains formaldehyde – induced neuronal damage in Prefrontal cortex of rats. Cell Biochem Funct 2006; 24 (3): 237-244.
• 6. Zararsız İ, Sönmez MF, Yılmaz HR, et al. Effects of omega - 3 essential fatty acids agains formaldehyde induced nephropathy in rats. Toxicol Ind Health 2006; 22 (5): 223-229.
• 7. Bourre JM, Bonneil M, Chaudiere J, Clement M, Dumont O, Durant, G. Structural and functional importance of dietary polyunsaturated fatty acids in the nervous system. Adv Exp Med Bio 1992; 318: 211-229.
• 8. Gomoh S, Hashimoto M, Sugioka K, Shahdat Hossain M, Hata N, Misawa Y, Masumura S. Cronic administration of docosahexaenoic acid improves reference memory – related learning ability in young rats. Neuroscience 1999; 93 (1): 237-241.
• 9. Sarsılmaz M, Songur A, Ozyurt H, et al. Potential role of dieterty omega – 3 essential fatty acids on same oxidant/antioxidant parameters in rats corpus striatum. Prostaglandins Leukot Essent Fatty Acids 2003; 69 (4): 131-135.
• 10. Stone NJ. Fish comsumption, fish oil, lipids and coronary heart disease. Am J Clin Nutr 1997; 65: 1083-1086.
• 11. Ramesh G, Das UN. Effects of free fatty acids on two-stage skin carcinogenesis in mice. Cancer Lett 1996; 100: 199-209.
• 12. Oosthuizen W, Vorster HH, Jerling JC, Bernard HC, Smuts CM, Silvis N, Kruger A, Venter CS. Both fish oil and olive oil lowered plasma fibrinojen in women with baseline fibrinogen levels. Thromb Haemostasis 1994; 72: 557-562.
• 13. Yılmaz HR, Songur A, Ozyurt B, Zararsız I, Sarsılmaz M. The effects of n – 3 polyunsaturated fatty acids by gavage on some metabolic enzymes of rat liver. Prostaglandins Leukot Essent Fatty Acids 2004; 71 (2): 131-135.
• 14. Saravanan P, Davidson NC, Schmidt EB, Calder PC. Cardiovascular effects of marine omega – 3 fatty acids. The Lancet 2010; 376 (9740): 540-550.
• 15. De Caterina RD, Enders S, Kristensen SD ve ark. N – 3 fatty acids and renal diseases. Am J Kidney Dis 1994; 24: 397-415.
• 16. Grannde JP, Walker HJ, Holub BJ ve ark. Suppresive effects of fish oil on mesengial cell proliferation in vitro and in vivo. Kidney int 2000; 57: 1027-1040.
• 17. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497-500.
• 18. Esterbauer H, Cheeseman KH: Determitation of aldehydic lipid peroxidation products: malonaldehyde and 4 – hidroxynonenal. Methods Enzymol 1990; 186: 407-421.
• 19. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterisation of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158-169.
• 20. Hallivel B. Free radicals, antioxidans and human disease; curiocity, course or consequence ? Lanced 1994; 344 (8924): 721-724.
• 21. Miyasaca CK, de souza JA, Torres RP, Filho JM, Lajolo FM, Curi R. Effect of the administration of fish oil by gavage on activities of antioxidant enzymes of rat lympohoid organs. Gen Pharmacol 1998; 30 (5): 759-762.
• 22. Roussea D, Tusseaint CH, Reaerstorff D ve ark. Dietary n – 3 polyunsatureted fatty acids affect the development of renovasculer hypertantion in rats. Molec Cell Biochem 2001; 225: 109 – 119.
• 23. Akyol Ö. Beyin tümörlerinde doku SOD, CAT ve GSH-Px aktiviteleri. Uzmanlık Tezi Ankara: Ankara Üniversitesi Tıp Fakültesi 1994.
• 24. Kamal AA, Gomaa A, el Khafif M, Hammad AS. Plasma lipid peroxides among workers exposed to silica or asbestos dust. Environ Res 1989; 49: 173-180.
• 25. Pompella A. Biochemistry and histochemistry of oxidant stress and lipid peroxidation. İnternational Journal for Vitamin and Nutrition Research 1997; 67: 289-297.
• 26. Kanter M, Coskun O, Korkmaz A, et al. Effects of nigella sativa on oxidative stress and beta – cell damage in streptozotocin – induced diabetic rats. The Anatomical Record. Part A, Discoveries in Molecular, Cellular and Evalutionary Biology 2004; 279: 685-691.
• 27. Song C, Zhang XY, Manku M. Increased phospholipase A2 activityand inflammatory responce but decreased nerve growth factor expression in the olfactory bulbectomized rat model of depression : effects of chronic ethyl – eicosapentaenoate treatment. J Neurosci 2009; 29: 14-22.
• 28. Zararsız İ, Sönmez MF, Yılmaz HR, ve ark. Sıçanlarda formaldehid uygulamasıyla akciğerlerde oluian hasar üzerine omega – 3 yağ asitlerinin koruyucu etkisi. Selçuk Tıp Dergisi 2004; 20: 93-98.
• 29. Pettersson EE, Rekola S, Berglund L, Sundqvist KG, Angelinm B, Diczfalusy U, et al. Treatment of Ig A nephropathy with omega – 3 polunsaturated fatty acids: a prospective, double-blind, randomized study. Clin Nephrol 1994; 41: 183-190.
• 30. MW Khan, S Priyamvada, SA Khan, S Khan ve ark. Protective effect of ω-3 polyunsaturated fatty acids (PUFAs) on sodium nitroprusside-induced nephrotoxicity and oxidative damege in rat kidney. Hum Exp Toxicol 2012; May 1: 1- 15.