Research Article
BibTex RIS Cite

SIÇANLARDA BEYİN PREFRONTAL KORTEKS DOKUSU ÜZERİNE OMEGA - 3 YAĞ ASİTLERİNİN KORUYUCU ETKİSİ

Year 2014, Volume: 3 Issue: 1, 22 - 26, 30.04.2014

Abstract

AMAÇ: Çalışmamızda prefrontal korteks dokusu üzerine omega - 3 yağ asitlerinin olumlu etkilerinin biyokimyasal düzeyde incelenmesi amaçlandı.


YÖNTEMLER: Çalışmamız esnasında kullanılan 16 adet Wistar - Albino cinsi erişkin erkek sıçan iki ayrı gruba ayrıldı. Grup I’deki sıçanlar kontrol grubu olarak değerlendirildi ve intargastrik gavaj ile serum fizyolojik gün aşırı zerk edildi. Grup II’deki sıçanlara ise 0.4 g/kg dozdaki omega - 3 yağ asiti intragastrik gavaj yolu ile her gün verildi. Altı hafta devam eden deney süresi sonunda hayvanlar dekapite edilerek prefrontal korteks dokuları çıkarıldı. Alınan prefrontal korteks dokusu örneklerine ait süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH – Px) enzim aktivasyonları ile malondialdehit (MDA) düzeyleri spektrofotometrik olarak belirlendi.


BULGULAR: Çalışmamız neticesinde omega - 3 yağ asiti verilen hayvanların prefrontal korteks dokularında SOD ve GSH – Px enzim aktiviteleri kontrol grubuna göre istatistiksel açıdan anlamlı bir artış gösterdi. MDA düzeyleri ise kontrol grubuna oranla anlamlı bir düşüş seyri gösterdi. SONUÇ: Sonuç olarak omega - 3 yağ asitlerinin beyin prefrontal korteks dokusundaki oksidatif hasara karşı önleyici bir etki yarattığı ve dolayısıyla bu doku üzerinde koruyucu bir işlev yürüttüğü tespit edildi.


References

  • 1. Storlien LH, Higgins JA, Thomas TCI. Diet composition and insülin action in animal models. Br J Nutr 2000; 83: 85-90.
  • 2. Mantzioris E, Cleland LG, Gibson RA. Biochemical effects of a diet containing foods enriched with n-3 fatty acids. Am J Clin Nutr 2000; 72: 42-48.
  • 3. Masters C. Omega - 3 fatty acids and the peroxisome. Mol Cell Biochem 1996; 165 (2): 83-93
  • 4. Salem N Jr, Litman B, Kim HY, Gawrish K. Mechanisms of docosahexaenoic acid in the nervous system. Lipids 2001; 36: 945-959.
  • 5. Piomelli D. Eicosanoids in synaptic transmission. Crit Rev Neurbiol 1994; 8: 65-83.
  • 6. Haag M. Essential fatty acids and the brain. Can J Psychiatry 2003; 48: 195-203
  • 7. Zararsız İ, Kuş İ, Akpolat N, Songur A, Ögetürk M, Sarsılmaz M. Protective effects of omega - 3 essential fatty acids agains formaldehyde – induced neuronal damage in Prefrontal cortex of rats. Cell Biochem Funct 2006; 24 (3): 237-244.
  • 8. Zararsız İ, Sönmez MF, Yılmaz HR, et al. Effects of omega - 3 essential fatty acids agains formaldehyde induced nephropathy in rats. Toxicol Ind Health 2006; 22 (5): 223-229.
  • 9. Wall R, Ross RP, Fitzgerald GF, Stanton C. Fatty acids from fish: the antiinflammatory potential of long - chain omega - 3 fatty acids. Nutr Rev 2010; 68: 280-289.
  • 10. Guest J, Garg M, Bilgin A, Grant R. Relationship between central and peripheral fatty acids in humans. Lipids Health Dis 2013; 12: 79.
  • 11. Konagai C, Yanagimoto K, Hayamizu K, Han L, Tsuji T, Koga Y. Effects of krill oil containing n-3 polyunsaturated fatty acids in phospholipid form on human brain function: a randomized controlled trial in healthy elderly volunteers. Clin Interv Aging 2013; 8: 1247-1257.
  • 12. Arıncı K, Elhan A. Anatomi, 2. Cilt, s.388, Güneş Kitapevi, Ankara (1995).
  • 13. Duncan J. Ana adaptive coding model of neural function in prefrontal cortex. Nat Rev Neurosci 2001; 2: 820-829.
  • 14. Nyberg L, Marklund P, Persson J, Cabeza R, Forkstam C, Peterss KM, Ingvar M. Common prefrontal activations during working memory, episodic memory and semantic memory. Neuropsychology 2003; 41: 371-377.
  • 15. Ganong WF. Tıbbi Fizyoloji, s. 249-268, Nobel Tıp Kitabevi, İstanbul (2002).
  • 16. Ranganath C, Jonson MK, D’ESpisoto M. Prefrontal activity associated with working memory and episodic long – term memory. Neuropsychologia 2003; 41: 378-389.
  • 17. Sarsılmaz M, Songur A, Ozyurt H, et al. Potential role of dieterty omega - 3 essential fatty acids on same oxidant/antioxidant parameters in rats corpus striatum. Prostaglandins Leukot Essent Fatty Acids 2003; 69 (4): 253-259.
  • 18. Stone NJ. Fish comsumption, fish oil, lipids and coronary heart disease. Am J Clin Nutr 1997; 65: 1083-1086.
  • 19. Miyasaca CK, de souza JA, Torres RP, Filho JM, Lajolo FM, Curi R. Effect of the administration of fish oil by gavage on activities of antioxidant enzymes of rat lympohoid organs. Gen Pharmacol 1998; 30 (5): 759-762.
  • 20. Tekzaki T, Inoue M, Kataoka H, et al. Diet and Lung canser risk from a 14 - year population-based prospective study in Japan with special reference of fish consumption. Nutr Canser 2003; 45: 160-167
  • 21. Kamal AA, Gomaa A, El Khafif M, Hammad AS. Plasma lipid peroxides among workers exposed to silica or asbestos dust. Environ Res 1989; 49: 173-180.
  • 22. Hallivel B. Free radicals, antioxidans and human disease; curiosity, course or consequence ? Lanced 1994; 344: 721-724.
  • 23. Akyol Ö. Beyin tümörlerinde doku SOD, CAT ve GSH-Px aktiviteleri. Uzmanlık Tezi Ankara Üniversitesi Tıp Fakültesi, Ankara 1994.
  • 24. Pompella A. Biochemistry and histochemistry of oxidant stress and lipid peroxidation. İnternational Journal for Vitamin and Nutrition Research 1997; 67: 289-297.
  • 25. Kanter M, Coskun O, Korkmaz A, et al. Effects of nigella sativa on oxidative stress and beta – cell damage in streptozotocin – induced diabetic rats. The Anatomical Record. Part A, Discoveries in Molecular, Cellular and Evalutionary Biology 2004; 279: 685-691.
  • 26. Song C, Zhang XY, Manku M. Increased phospholipase A2 activityand inflammatory responce but decreased nerve growth factor expression in the olfactory bulbectomized rat model of depression : effects of chronic ethyl – eicosapentaenoate treatment. J Neurosci 2009; 29: 14-22.
  • 27. Lonergan PE, Martin DS, Horrobin DF, Lynch MA. Neuroprotective effect of eicosapentaenoic acid in hippocampus of rats exposed to gamma - irradiation. J Biol Chem 2002; 277: 20804-20811.
  • 28. Martin DS, Lonergan PE, Boland B, Fogarty MP, Brady M. Apoptotic changes in the aged brain are triggered by interleukin - 1 beta - induced activation of p38 and reversed by treatment with eicosapentaenoic acid. J Biol Chem 2002; 277: 34239-34246.
  • 29. Nordoy A. Is there a rational use for n - 3 fatty asids (fish oils) in clinical medicine ?. Drugs 1991; 42: 331-342.

THE PROTECTIVE EFFECTS OF OMEGA - 3 FATTY ACIDS ON PREFRONTAL CORTEX OF BRAIN IN RATS

Year 2014, Volume: 3 Issue: 1, 22 - 26, 30.04.2014

Abstract


OBJECTIVE: In our study of prefrontal cortex on the positive effects of omega - 3 fatty acids was aimed to investigate the biochemical level.



METHODS: During our study used 16 Wistar - Albino male rats in the two groups were divided. Rats in Group I are used as control group and they are only intragastric gavaged with normal saline. Rats in group II, 0.4 g / kg dose of omega - 3 fatty acid was given every day by intragastric gavage. At the end of six weeks the ongoing experiments animals were decapitated and prefrontal cortex were removed. Get in the prefrontal cortex of superoxide dismutase (SOD) and glutathione peroxidase (GSH - Px) enzyme activation and malondialdehyde (MDA) levels were determined spectrophotometrically.



RESULTS: As a result of our study of omega - 3 fatty acids in the prefrontal cortex of animals SOD and GSH - Px enzyme activity compared to the control group showed a statistically significant increase. MDA levels compared to the control group showed a significant downward trend.

CONCLUSION: As a result, omega - 3 fatty acids in the prefrontal cortex of the brain, a preventive effect against oxidative damage and therefore conducted a protective function on these tissues were detected.


References

  • 1. Storlien LH, Higgins JA, Thomas TCI. Diet composition and insülin action in animal models. Br J Nutr 2000; 83: 85-90.
  • 2. Mantzioris E, Cleland LG, Gibson RA. Biochemical effects of a diet containing foods enriched with n-3 fatty acids. Am J Clin Nutr 2000; 72: 42-48.
  • 3. Masters C. Omega - 3 fatty acids and the peroxisome. Mol Cell Biochem 1996; 165 (2): 83-93
  • 4. Salem N Jr, Litman B, Kim HY, Gawrish K. Mechanisms of docosahexaenoic acid in the nervous system. Lipids 2001; 36: 945-959.
  • 5. Piomelli D. Eicosanoids in synaptic transmission. Crit Rev Neurbiol 1994; 8: 65-83.
  • 6. Haag M. Essential fatty acids and the brain. Can J Psychiatry 2003; 48: 195-203
  • 7. Zararsız İ, Kuş İ, Akpolat N, Songur A, Ögetürk M, Sarsılmaz M. Protective effects of omega - 3 essential fatty acids agains formaldehyde – induced neuronal damage in Prefrontal cortex of rats. Cell Biochem Funct 2006; 24 (3): 237-244.
  • 8. Zararsız İ, Sönmez MF, Yılmaz HR, et al. Effects of omega - 3 essential fatty acids agains formaldehyde induced nephropathy in rats. Toxicol Ind Health 2006; 22 (5): 223-229.
  • 9. Wall R, Ross RP, Fitzgerald GF, Stanton C. Fatty acids from fish: the antiinflammatory potential of long - chain omega - 3 fatty acids. Nutr Rev 2010; 68: 280-289.
  • 10. Guest J, Garg M, Bilgin A, Grant R. Relationship between central and peripheral fatty acids in humans. Lipids Health Dis 2013; 12: 79.
  • 11. Konagai C, Yanagimoto K, Hayamizu K, Han L, Tsuji T, Koga Y. Effects of krill oil containing n-3 polyunsaturated fatty acids in phospholipid form on human brain function: a randomized controlled trial in healthy elderly volunteers. Clin Interv Aging 2013; 8: 1247-1257.
  • 12. Arıncı K, Elhan A. Anatomi, 2. Cilt, s.388, Güneş Kitapevi, Ankara (1995).
  • 13. Duncan J. Ana adaptive coding model of neural function in prefrontal cortex. Nat Rev Neurosci 2001; 2: 820-829.
  • 14. Nyberg L, Marklund P, Persson J, Cabeza R, Forkstam C, Peterss KM, Ingvar M. Common prefrontal activations during working memory, episodic memory and semantic memory. Neuropsychology 2003; 41: 371-377.
  • 15. Ganong WF. Tıbbi Fizyoloji, s. 249-268, Nobel Tıp Kitabevi, İstanbul (2002).
  • 16. Ranganath C, Jonson MK, D’ESpisoto M. Prefrontal activity associated with working memory and episodic long – term memory. Neuropsychologia 2003; 41: 378-389.
  • 17. Sarsılmaz M, Songur A, Ozyurt H, et al. Potential role of dieterty omega - 3 essential fatty acids on same oxidant/antioxidant parameters in rats corpus striatum. Prostaglandins Leukot Essent Fatty Acids 2003; 69 (4): 253-259.
  • 18. Stone NJ. Fish comsumption, fish oil, lipids and coronary heart disease. Am J Clin Nutr 1997; 65: 1083-1086.
  • 19. Miyasaca CK, de souza JA, Torres RP, Filho JM, Lajolo FM, Curi R. Effect of the administration of fish oil by gavage on activities of antioxidant enzymes of rat lympohoid organs. Gen Pharmacol 1998; 30 (5): 759-762.
  • 20. Tekzaki T, Inoue M, Kataoka H, et al. Diet and Lung canser risk from a 14 - year population-based prospective study in Japan with special reference of fish consumption. Nutr Canser 2003; 45: 160-167
  • 21. Kamal AA, Gomaa A, El Khafif M, Hammad AS. Plasma lipid peroxides among workers exposed to silica or asbestos dust. Environ Res 1989; 49: 173-180.
  • 22. Hallivel B. Free radicals, antioxidans and human disease; curiosity, course or consequence ? Lanced 1994; 344: 721-724.
  • 23. Akyol Ö. Beyin tümörlerinde doku SOD, CAT ve GSH-Px aktiviteleri. Uzmanlık Tezi Ankara Üniversitesi Tıp Fakültesi, Ankara 1994.
  • 24. Pompella A. Biochemistry and histochemistry of oxidant stress and lipid peroxidation. İnternational Journal for Vitamin and Nutrition Research 1997; 67: 289-297.
  • 25. Kanter M, Coskun O, Korkmaz A, et al. Effects of nigella sativa on oxidative stress and beta – cell damage in streptozotocin – induced diabetic rats. The Anatomical Record. Part A, Discoveries in Molecular, Cellular and Evalutionary Biology 2004; 279: 685-691.
  • 26. Song C, Zhang XY, Manku M. Increased phospholipase A2 activityand inflammatory responce but decreased nerve growth factor expression in the olfactory bulbectomized rat model of depression : effects of chronic ethyl – eicosapentaenoate treatment. J Neurosci 2009; 29: 14-22.
  • 27. Lonergan PE, Martin DS, Horrobin DF, Lynch MA. Neuroprotective effect of eicosapentaenoic acid in hippocampus of rats exposed to gamma - irradiation. J Biol Chem 2002; 277: 20804-20811.
  • 28. Martin DS, Lonergan PE, Boland B, Fogarty MP, Brady M. Apoptotic changes in the aged brain are triggered by interleukin - 1 beta - induced activation of p38 and reversed by treatment with eicosapentaenoic acid. J Biol Chem 2002; 277: 34239-34246.
  • 29. Nordoy A. Is there a rational use for n - 3 fatty asids (fish oils) in clinical medicine ?. Drugs 1991; 42: 331-342.
There are 29 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Burak Gülcen This is me

Ömür Karaca This is me

Murat Abdulgani Kuş This is me

Emrah Özcan

Dilara Kaman This is me

Murat Ögetürk This is me

İlter Kuş This is me

Publication Date April 30, 2014
Submission Date November 12, 2013
Published in Issue Year 2014 Volume: 3 Issue: 1

Cite

APA Gülcen, B., Karaca, Ö., Kuş, M. A., Özcan, E., et al. (2014). SIÇANLARDA BEYİN PREFRONTAL KORTEKS DOKUSU ÜZERİNE OMEGA - 3 YAĞ ASİTLERİNİN KORUYUCU ETKİSİ. Balıkesir Sağlık Bilimleri Dergisi, 3(1), 22-26.

International Peer Reviewed Journal

The journal adopts Open Access Policy and does not request article proccessing charge (APC), article publishing charge or any other charges.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.