TİCARİ YUMURTACI TAVUK SÜRÜLERİNDE TERMOFİLİK CAMPYLOBACTER SPP. PREVALANSI VE ANTİBİYOTİK DUYARLILIKLARI

Year 2015, Volume: 4 Issue: 2, 85 - 90, 31.08.2015

Yavuz Çokal

Abstract

AMAÇ: Bu çalışmada, Bandırma’da bulunan ticari yumurtacı tavuk sürülerinde termofilik Campylobacter spp. prevalansının ve elde edilen izolatların değişik antibiyotiklere duyarlılıklarının belirlenmesi amaçlandı.

YÖNTEMLER: Ağustos 2013-Şubat 2014 döneminde, 9 adet ticari yumurtacı tavuk kümesi ziyaret edildi ve toplam 362 adet taze dışkı örneği alındı. Dışkı örneklerinden Modified Charcoal Cefaperazone Deoxycholate Agar’a direkt ekimler yapıldı ve mikroaerofilik koşullarda 42°C’de 48 saat inkübe edildi. Campylobacter şüpheli koloniler morfolojik ve biyokimyasal karakterlerine göre identifiye edildi. İzolatların antibiyotiklere duyarlılıkları agar disk difüzyon yöntemi ile belirlendi.

BULGULAR: Bakteriyolojik analiz sonucunda, 9 kümesin tamamından (%100) ve örneklerin %39,8’inden Campylobacter spp. izolasyonu gerçekleşti. İzole edilen 144 adet Campylobacter izolatının 88’i C. jejuni (%61,1) ve 56’sı C. coli (%38,9) olarak identifiye edildi. Antibiyotik duyarlılık test sonuçlarına göre, C. jejuni ve C. coli izolatlarının tetrasikline sırasıyla %46,6 ve %50 oranlarında dirençli oldukları bulundu. Florokinolonlara (siprofloksasin ve enrofloksasin) direnç oranlarının ise sırasıyla C. jejuni izolatlarında %62,5 ve %27,3, C. coli izolatlarında %82,1 ve %71,4 olduğu tespit edildi.

SONUÇ: Termofilik Campylobacter türlerinin ticari yumurtacı sürülerde yüksek prevalansa sahip olduğu belirlendi. Ayrıca, C. jejuni ve C. coli izolatlarının tetrasiklin, siprofloksasin ve enrofloksasine yüksek oranda dirençli oldukları tespit edildi.

Keywords

Campylobacter spp., Ticari yumurtacı tavuk, Prevalans

THE PREVALENCE AND ANTIBIOTIC SUSCEPTIBILITIES OF THERMOPHILIC CAMPYLOBACTER SPP. IN COMMERCIAL LAYER FLOCKS

Abstract

OBJECTIVE: This study was aimed to determine the prevalence and antibiotic susceptibilities of thermophilic Campylobacter spp. in commercial layer flocks in Bandirma.

METHODS: Nine commercial layer flocks were visited between August 2013 and February 2014, and a total of 362 fresh fecal specimens were collected from chickens. Fecal specimens were inoculated onto the Modified Charcoal Cefaperazone Deoxycholate Agar and incubated at 42 °C for 48 h. Colonies suspected for Campylobacter spp. were identified at species level by morphological and biochemical characters. The antibiotic susceptibilities of the isolates were determined by using the disk diffusion method.

RESULTS: As a result of the bacteriological analysis, Campylobacter spp. was isolated from all flocks (100%) and 39,8% of all the analysed samples. Eighty eight of 144 Campylobacter isolates C. jejuni (61,1%) and 56 of 144 Campylobacter isolates C. coli (38,9%) were identified. According to the results of antibiotic susceptibility test, the isolates of C. jejuni and C. coli were found to be resistant to tetracycline at the rates of 46,6% and 50% respectively. Resistance rates to the fluoroquinolones (ciprofloxacin and enrofloxacin) were found to be 62,5% and 27,3% for C. jejuni isolates and to be 82,1% and 71,4% for C. coli isolates, respectively

CONCLUSION: The prevalence of thermophilic Campylobacter spp. was determined to be high in commercial layer flocks. Also, it was found that the isolates of C. jejuni and C. coli are highly resistant to tetracycline, ciprofloxacin and enrofloxacin.

Keywords

Campylobacter spp., Commercial layer flock

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