Sisplatinin Neden Olduğu Testikular Oksidatif Stres Üzerine Kı̇sspeptı̇n-10'un Etkisi

Year 2025, Volume: 14 Issue: 1, 184 - 191, 28.03.2025

Gözde Arkalı , Tutku Can Acısu , Edanur Güler Ekmen , Meltem Sağıroğlu , Fatma Beril Koçyiğit , Mehmet Çay , Abdurrauf Yüce , Mesut Aksakal

Abstract

Amaç: Antikanserojen ilaçlardan biri olan sisplatin, spermatogenik hücrelerde, Sertoli hücrelerinde, Leydig hücrelerinde hasara yol açmaktadır. Bu çalışmada, sisplatinin erkek üreme organlarında neden olduğu oksidatif stres ve akabinde gelişen spermatolojik parametreler üzerindeki olumsuz etkilere karşı kisspeptin-10’un etkisinin araştırılması amaçlandı. Gereç ve Yöntem: Deneyde 34 adet erkek Sprague Dawley sıçanı kullanıldı. Ratlar kontrol, sisplatin, Kisspeptin-10 ve sisplatin+kisspeptin-10 olarak 4 gruba ayrıldı. Sisplatin 5 mg/kg/tek doz, Kisspeptin-10 ise 50 nmol/kg dozda 7 gün boyunca intraperitoneal uygulandı. Bulgular: Sisplatin grubunda testikular malondialdehit düzeyinin arttığı (p<0.001), katalaz enzim aktivitesinin (p<0.001) ve glutatyon düzeyinin (p<0.01) azaldığı, sperm motilitesinin (p<0.05) ve testis (p<0.01) ile vezikula seminalis (p<0.001) üreme organ ağırlığının azaldığı belirlendi. CP grubu ile kıyaslandığında CP+Kiss-10 grubunda testikular malondialdehit düzeyinin azaldığı (p<0.001), glutatyon düzeyinin arttığı (p<0.01), sperm motilitesinin (p<0.05) ve testis (p<0.01) ile vezikula seminalis (p<0.001) üreme organ ağırlığının arttığı belirlendi. Sonuç: Kisspeptin-10’un sisplatin kaynaklı testikular hasarda etkili olan oksidatif stresi azaltarak, spermatolojik parametreler üzerinde iyileştirici etkiler gösterdiği kanısına varılmıştır.

Keywords

Sisplatin, Testis, Kisspeptin-10, Oksidatif Stres

The Effect of Kisspeptin-10 on Cisplatin-Induced Testicular Oxidative Stress

Abstract

Objective: Cisplatin, one of the anticarcinogenic drugs, causes damage to spermatogenic cells, sertoli cells and leydig cells. This study aimed to investigate the kisspeptin-10 effect against oxidative stress caused by cisplatin in male reproductive organs and its negative effects on spermatological parameters. Materials and Methods: In the experiment, 34 male Sprague Dawley rats were used. Rats were divided into 4 groups as control, cisplatin, Kisspeptin-10 and cisplatin+kisspeptin-10. Cisplatin 5 mg/kg/single dose and Kisspeptin-10 50 nmol/kg dose were administered intraperitoneally for 7 days. Results: In the cisplatin group, testicular malondialdehyde level increased (p<0.001), catalase enzyme activity (p<0.001) and glutathione level (p<0.01) decreased, sperm motility (p<0.05) and testicular (p<0.01) and seminal vesicle (p<0.001) weights decreased. In the cisplatin group administered kisspeptin-10, it was determined that testicular malondialdehyde level decreased (p<0.001), glutathione level increased (p<0.01), sperm motility increased (p<0.05), and testicular (p<0.01) and seminal vesicle (p<0.001) weights increased compared to the cisplatin group. Conclusion: As a result, it was revealed that Kisspeptin-10 showed improving effects on spermatological parameters by reducing oxidative stress that is effective in cisplatin-induced testicular damage.

Keywords

Cisplatin, Testes, Kisspeptin-10, Oxidative Stress

Ethical Statement

Ethics Committee Approval: This study was carried out at Fırat University Experimental Research Center with the “permission dated 16.03.2022 and numbered 2022/05” of the Fırat University Animal Experiments Local Ethics Committee

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