Erzurum Bölgesinde Yetişen Betula pendula Yapraklarının Antioksidan Kapasitelerinin Belirlenmesi ve Kemometrik Karakterizasyonu

Year 2019, Volume: 21 Issue: 1, 147 - 154, 15.04.2019

Onur Şenol

https://doi.org/10.24011/barofd.517817

Abstract

Bu çalışmada,  Erzurum bölgesinin
karakteristik bitki örtüsü içerisinde yer alan Huş Ağacına ait farklı
yerleşimlerden alınan yaprak örnekleri farklı in vitro antioksidan kapasite
yöntemleri ile incelenmiş ve kemometrik karakterizasyonu yapılmıştır. Uzundere,
Horasan ve Şenkaya bölgelerinde mayıs-temmuz aralığında toplanan örnekler
kurutulmuş ve etanol ekstreleri çıkarılmıştır. Bakır II iyonu İndirgeyici Antioksidan
Kapasite (CUPRAC), Demir III iyonu İndirgeyici Antioksidan Kapasite (FRAP) ve
Difenil pikril Hidrazin (DPPH) yöntemleri için gerçekleştirilen analizlere
bütün örnekler pozitif yanıt vermişlerdir. En yüksek antioksidan kapasite
değerlerine Şenkaya bölgesinden elde edilen örneklerle ulaşılmıştır. Elde
edilen değerler bilgisayar algoritmaları yardımıyla kemometrik olarak
incelendiğinde; sonuçların anlamlı farklılık gösterdiği ve her bölgede rakım
değişimiyle pozitif korelasyon gösteren farklılıklar tespit edilmiştir. Yapılan
çalışma vasıtasıyla antioksidan kapasite değerleri ile coğrafi
karakterizasyonun gerçekleştirilebileceği ve rakımsal farklılıkların Huş ağacı
yapraklarının fenolik ve flavanoid içeriğine anlamlı etki yaptığı sonucuna
varılmıştır. Huş ağacı bitkisinin antioksidan kapasitesi sebebiyle özellikle
kanser ve kozmetik gibi önemli alanlarda kullanılabileceği düşünülmektedir.

Keywords

Antioksidan Kapasite, Huş Ağacı Yaprağı, CUPRAC, FRAP, DPPH

Determination of Antioxidant Activity and Chemometric Characterization of Betula pendula Leaves in Erzurum Region

Abstract

In this study, leaf samples of Birch Tree collected from different
settlements in the characteristic vegetation of Erzurum region were
investigated with different in vitro antioxidant activity investigated and
chemometrics characterization was carried out. The samples were harvested in
the May-July period from Uzundere, Horasan and Şenkaya regions,  dried and their ethanol extracts were
obtained. CUPRAC, FRAP and DPPH responses were positive for all samples. The
highest antioxidant capacity values ​​were obtained
from samples of Şenkaya region. When the obtained values ​​are examined chemometrically with the help of
computer algorithms; significant differences and a positive correlation with
the altitude change in each region were obtained. It was concluded that
geographic characterization can be realized by antioxidant activity values ​​and the altitude differences have a significant
effect on phenolic and flavanoid contents of birch leaves. Owing to the
antioxidant activity of the birch plant it is thought that it can be used
especially in important areas such as cancer and cosmetics.

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