Dağ Çayı (Sideritis condensata Boiss. Et. Heldr. Apud Bentham) ’ın Doğal ve Tarla Ortamındaki Uçucu Bileşenlerinin Belirlenmesi

Year 2023, Volume: 25 Issue: 3, 411 - 420, 15.12.2023

Güliz Türkmenoğlu , Hüseyin Fakir

https://doi.org/10.24011/barofd.1248664

Abstract

Bu çalışmada doğadan toplanan ve tarla koşullarında yetiştirilen Sideritis condensata Boiss. Et. Heldr. Apud Bentham türü dağ çayının uçucu bileşenlerinin HS-SPME GC-MS tekniği ile karşılaştırılması amaç-lanmıştır. Bitkinin çelikleri Isparta İli, Yenişarbademli İlçesi, Dedegöl Dağı kayalık mevkiinden 2020 yılı Nisan ayında toplanmış ve sera ortamında köklendirilerek aynı yıl Mayıs ayı sonunda tarla koşullarına aktarılmıştır. 2021 yılı Haziran-Temmuz ayında (çiçeklenme dönemi) hem tarla hem de doğal koşullardan türe ait yaprak ve çiçek örnekleri toplanmış ve oda koşullarında kurutulmuştur. Katı faz mikro ekstraksi-yon tekniği kullanılarak, gaz kromatografisi kütle spektrometresi (GC-MS) ile S. condensata 'da doğal yetişme ortamında 72 adet uçucu bileşen ve tarla ortamında 88 adet uçucu bileşen belirlenmiştir. Doğal yetişme ortamı ve tarla ortamındaki uçucu bileşenlerinden α-pinene (9.30%-2.32%), β-pinene (18.12%-5.66%), β-ocimene (4.46%-1.06%), trans-caryophyllene (12.77%-0.83%), germacrene-D (10.59%-0.67%), γ-cadinene (2.09%-10.94%), α-gurjunene (0.03%-16.68%), farnesene (0.98%-6.00%) Sideritis condensata 'nın ana bileşenleri olarak tespit edilmiştir. Uçucu bileşen sınıflarına bakıldığında seskiterpen hidrokarbon-ların yüksek oranda olduğu bulunduğu görülmüştür.

Keywords

Sideritis condensata, katı faz mikro ekstraksiyon tekniği, endemik, uçucu bileşen, β-pinene, α- pinene, germacrene-D

Project Number

. 2021-D1-0132 No

Determination of Volatile Components in Mountain Tea (Sideritis condensata Boiss. Et. Heldr. Apud Bentham) Grown in its Natural and Agricultural Field Environment

Abstract

In this study, it was aimed to compare the volatile components of Sideritis condensata Boiss. Et. Heldr. Apud Bentham species mountain tea collected from nature and grown under field conditions with HS-SPME GC-MS technique. The cuttings of the plant were collected from Isparta Province, Yenişarbademli District, rocky location of Dedegöl Mountain in April 2020, and were transferred to field conditions at the end of May in the same year after rooted in a greenhouse environment. In June-July 2021, during the flowering period, leaf and flower samples peculiar to the species were collected from both field and natural environments and dried under room conditions. Using solid based micro-extraction technique, 72 volatile components were determined in natural growing environment and 88 volatile components in field envi-ronment in S. condensata by gas chromatography mass spectrometry (GC-MS). Among the volatile com-ponents found in natural habitat and field environment, α-pinene (9.30%-2.32%), β-pinene (18.12%-5.66%), β-ocimene (4.46%-1.06%), trans-caryophyllene (12.77%-0.83%), germacrene-D (10.59%-0.67%), γ-cadinene (2.09%-10.94%), α-gurjunene (0.03%-16.68%), farnesene (0.98%-6.00%) were identified as the main components of Sideritis condensata. When the volatile components were examined, it was obser-ved that sesquiterpene hydrocarbons were high.

Keywords

Sideritis condensata, solid-based micro extraction technique, endemic, volatile component, β-pinene, α- pinene, germacrene-D

Supporting Institution

Isparta Uygulamalı Bilimler Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi

Project Number

. 2021-D1-0132 No

Thanks

This study was produced from a part of the PhD Thesis titled “ Determination of some Botanical Characteristic and Agricultural Performances of Mountain Tea (Sideritis spp.) TaxaSpreading Naturally in Isparta Province” Isparta University of Applied Sciences, Graduate Education Institute. We are deeply grateful to Isparta University of Applied Sciences Scientific Research Projects Management Unit, who financially supported the thesis with the Project number 2021-D1-0132.

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