Combinations of essential oils obtained from medicinal plants with L. angustifolia: Determination of their antimicrobial effects

Derya Doğanay; Şevval Maral Özcan Aykol; G. Vildan Seyhan; İlker Demirbolat; Esra Mertoğlu

doi:10.53445/batd.1789276

EN TR

Combinations of essential oils obtained from medicinal plants with L. angustifolia: Determination of their antimicrobial effects

Abstract

In this study, the antimicrobial effects of essential oils obtained from 7 different plants (Lavandula angustifolia, Lavandula intermedia, Cupressus sempervirens, Syzygium aromaticum, Salvia officinalis, Allium sativum, Pimpinella anisum) and their combinations with Lavandula angustifolia, were investigated against 11 pathogenic bacteria (Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 29213, Staphylococcus haemolyticus ATCC 43252, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603, Escherichia coli ATCC NRRLB 3704, Acinetobacter baumannii ATCC 19606, Bacillus subtilis ATCC 6633, Proteus vulgaris ATCC 13315, Enterococcus faecalis ATCC 29212, Aeromonas hydrophila ATCC 95080). The synergistic effect of essential oils and their combinations with L. angustifolia were determined using the agar well diffusion method. Each essential oil was subjected to gas chromatography analysis in order to identify their chemical compositions. According to the results, generally all essential oils exhibited the greatest inhibition zones for Gram-positive bacteria than for Gram-negative bacteria. Among the essential oil combinations, the highest synergistic effect (166.7%) was obtained in L. angustifolia-P.anisum combination against P. aeruginosa ATCC 27853. Additionally, the L. angustifolia-S. aromaticum combination was observed to exhibit a synergistic effect on 5 bacteria (K. pneumoniae, E. coli, E. faecalis, S. haemolyticus and A. hydrophila). These findings suggest that L. angustifolia combined with specific essential oils particularly L. intermedia and S. aromaticum may serve as promising natural antimicrobial agents, offering enhanced inhibitory activity against clinically important bacterial pathogens.

Keywords

Essential oils, antibacterial activity, agar well diffusion method, synergistic effect, pathogen bacteria, inhibition zone

Tıbbi bitkilerden elde edilen uçucu yağların L. angustifolia ile kombinasyonları: Antimikrobiyal etkilerinin belirlenmesi

Abstract

Bu çalışmada 7 farklı bitkiye (Lavandula angustifolia, Lavandula intermedia, Cupressus sempervirens, Syzygium aromaticum, Salvia officinalis, Allium sativum, Pimpinella anisum) ait uçucu yağın ve bunların Lavandula angustifolia ile kombinasyonlarının 11 patojen bakteriye (Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 29213, Staphylococcus haemolyticus ATCC 43252, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603, Escherichia coli ATCC NRRLB 3704, Acinetobacter baumannii ATCC 19606, Bacillus subtilis ATCC 6633, Proteus vulgaris ATCC 13315, Enterococcus faecalis ATCC 29212, Aeromonas hydrophila ATCC 95080) karşı antimikrobiyal etkileri araştırılmıştır. Uçucu yağların ve L. angustifolia ile kombinasyonlarının sinerjik etkisi agar kuyu difüzyon yöntemi ile belirlenmiştir. Her bir uçucu yağ, kimyasal bileşimlerini belirlemek amacıyla gaz kromatografisi analizine tabi tutulmuştur. Sonuçlara göre genel olarak test edilen tüm uçucu yağlar Gram-pozitif bakteriler için Gram-negatif bakterilerden daha büyük inhibisyon zonu göstermiştir. Uçucu yağ kombinasyonları arasında en yüksek sinerjik etki (166,7%) Pseudomonas aeruginosa ATCC 27853’e karşı L. angustifolia-P. anisum kombinasyonunda elde edilmiştir. Ayrıca L. angustifolia-S. aromaticum kombinasyonunun 5 bakteri (K. pneumoniae, E. coli, E. faecalis, S. haemolyticus ve A. hydrophila) üzerinde sinerjik etki gösterdiği gözlenmiştir. Bu bulgular, L. angustifolia’nın belirli uçucu yağlarla (özellikle L. intermedia ve S. aromaticum) birlikte kullanıldığında, klinik açıdan önemli bakteriyel patojenlere karşı gelişmiş inhibitör aktivite sunarak umut verici doğal antimikrobiyal ajanlar olarak işlev görebileceğini düşündürmektedir.

Keywords

Uçucu yağlar, antibakteriyel aktivite, agar kuyu difüzyon yöntemi, sinerjik etki, patojen bakteri, inhibisyon bölgesi

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Details

Primary Language

English

Subjects

Botany (Other), Environmental Health

Journal Section

Research Article

Authors

Derya Doğanay ^*
0000-0001-9147-4110
Türkiye

Şevval Maral Özcan Aykol
0000-0002-3336-5618
Türkiye

G. Vildan Seyhan
0000-0001-9162-2937
Türkiye

İlker Demirbolat
0000-0002-8756-1884
Türkiye

Esra Mertoğlu
0009-0005-5759-0958
Türkiye

Publication Date

December 30, 2025

Submission Date

September 24, 2025

Acceptance Date

December 16, 2025

Published in Issue

Year 2025 Volume: 6 Number: 3

DOI

https://doi.org/10.53445/batd.1789276

IZ

https://izlik.org/JA63ST86RN

APA

Doğanay, D., Özcan Aykol, Ş. M., Seyhan, G. V., Demirbolat, İ., & Mertoğlu, E. (2025). Combinations of essential oils obtained from medicinal plants with L. angustifolia: Determination of their antimicrobial effects. Journal of Integrative and Anatolian Medicine, 6(3), 175-185. https://doi.org/10.53445/batd.1789276

AMA

1.Doğanay D, Özcan Aykol ŞM, Seyhan GV, Demirbolat İ, Mertoğlu E. Combinations of essential oils obtained from medicinal plants with L. angustifolia: Determination of their antimicrobial effects. J. Integr. Anatol. Med. 2025;6(3):175-185. doi:10.53445/batd.1789276

Chicago

Doğanay, Derya, Şevval Maral Özcan Aykol, G. Vildan Seyhan, İlker Demirbolat, and Esra Mertoğlu. 2025. “Combinations of Essential Oils Obtained from Medicinal Plants With L. Angustifolia: Determination of Their Antimicrobial Effects”. Journal of Integrative and Anatolian Medicine 6 (3): 175-85. https://doi.org/10.53445/batd.1789276.

EndNote

Doğanay D, Özcan Aykol ŞM, Seyhan GV, Demirbolat İ, Mertoğlu E (December 1, 2025) Combinations of essential oils obtained from medicinal plants with L. angustifolia: Determination of their antimicrobial effects. Journal of Integrative and Anatolian Medicine 6 3 175–185.

IEEE

[1]D. Doğanay, Ş. M. Özcan Aykol, G. V. Seyhan, İ. Demirbolat, and E. Mertoğlu, “Combinations of essential oils obtained from medicinal plants with L. angustifolia: Determination of their antimicrobial effects”, J. Integr. Anatol. Med., vol. 6, no. 3, pp. 175–185, Dec. 2025, doi: 10.53445/batd.1789276.

ISNAD

Doğanay, Derya - Özcan Aykol, Şevval Maral - Seyhan, G. Vildan - Demirbolat, İlker - Mertoğlu, Esra. “Combinations of Essential Oils Obtained from Medicinal Plants With L. Angustifolia: Determination of Their Antimicrobial Effects”. Journal of Integrative and Anatolian Medicine 6/3 (December 1, 2025): 175-185. https://doi.org/10.53445/batd.1789276.

JAMA

1.Doğanay D, Özcan Aykol ŞM, Seyhan GV, Demirbolat İ, Mertoğlu E. Combinations of essential oils obtained from medicinal plants with L. angustifolia: Determination of their antimicrobial effects. J. Integr. Anatol. Med. 2025;6:175–185.

MLA

Doğanay, Derya, et al. “Combinations of Essential Oils Obtained from Medicinal Plants With L. Angustifolia: Determination of Their Antimicrobial Effects”. Journal of Integrative and Anatolian Medicine, vol. 6, no. 3, Dec. 2025, pp. 175-8, doi:10.53445/batd.1789276.

Vancouver

1.Derya Doğanay, Şevval Maral Özcan Aykol, G. Vildan Seyhan, İlker Demirbolat, Esra Mertoğlu. Combinations of essential oils obtained from medicinal plants with L. angustifolia: Determination of their antimicrobial effects. J. Integr. Anatol. Med. 2025 Dec. 1;6(3):175-8. doi:10.53445/batd.1789276

J. Integr. Anatol. Med. (Abbreviated Title)

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e-ISSN: 2687-6345

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This work is licensed under Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0).

Combinations of essential oils obtained from medicinal plants with L. angustifolia: Determination of their antimicrobial effects

Abstract

Keywords

Tıbbi bitkilerden elde edilen uçucu yağların L. angustifolia ile kombinasyonları: Antimikrobiyal etkilerinin belirlenmesi

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

J. Integr. Anatol. Med. (Abbreviated Title)