Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin  in Reversed-phase Liquid  Chromatography

A. Pappa Louisi; P. Agrafiotou; G. Zissopoulou; P. Liatsi

EN

Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin in Reversed-phase Liquid Chromatography

Abstract

The reversed-phase (RP) chromatographic behaviour of the macrolide antibiotics clarithromycin (Clari) and roxithromycin (Roxi) was extensively studied as a function of mobile phase composition - modified with one, two or three of organic solvents - and column temperature. The results of our study provide a satisfactory thermodynamic explanation for the retention processes of these macrolides in RP-HPLC and give additional information on the selection of optimal separation conditions for the determination of Clari in human plasma using Roxi as an internal standard and vice versa

Keywords

clarithromycin, roxithromycin, HPLC retention

Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

A. Pappa Louisi This is me

P. Agrafiotou This is me

G. Zissopoulou This is me

P. Liatsi This is me

Publication Date

December 1, 2002

Submission Date

December 1, 2002

Acceptance Date

-

Published in Issue

Year 2002 Volume: 4 Number: 2

IZ

https://izlik.org/JA43GH63MT

Cite

RIS / Bibtex

APA

Pappa Louisi, A., Agrafiotou, P., Zissopoulou, G., & Liatsi, P. (2002). Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin in Reversed-phase Liquid Chromatography. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 4(2), 17-19. https://izlik.org/JA43GH63MT

AMA

1.Pappa Louisi A, Agrafiotou P, Zissopoulou G, Liatsi P. Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin in Reversed-phase Liquid Chromatography. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2002;4(2):17-19. https://izlik.org/JA43GH63MT

Chicago

Pappa Louisi, A., P. Agrafiotou, G. Zissopoulou, and P. Liatsi. 2002. “Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin in Reversed-Phase Liquid Chromatography”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 4 (2): 17-19. https://izlik.org/JA43GH63MT.

EndNote

Pappa Louisi A, Agrafiotou P, Zissopoulou G, Liatsi P (December 1, 2002) Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin in Reversed-phase Liquid Chromatography. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 4 2 17–19.

IEEE

[1]A. Pappa Louisi, P. Agrafiotou, G. Zissopoulou, and P. Liatsi, “Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin in Reversed-phase Liquid Chromatography”, Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 4, no. 2, pp. 17–19, Dec. 2002, [Online]. Available: https://izlik.org/JA43GH63MT

ISNAD

Pappa Louisi, A. - Agrafiotou, P. - Zissopoulou, G. - Liatsi, P. “Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin in Reversed-Phase Liquid Chromatography”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 4/2 (December 1, 2002): 17-19. https://izlik.org/JA43GH63MT.

JAMA

1.Pappa Louisi A, Agrafiotou P, Zissopoulou G, Liatsi P. Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin in Reversed-phase Liquid Chromatography. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2002;4:17–19.

MLA

Pappa Louisi, A., et al. “Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin in Reversed-Phase Liquid Chromatography”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 4, no. 2, Dec. 2002, pp. 17-19, https://izlik.org/JA43GH63MT.

Vancouver

1.A. Pappa Louisi, P. Agrafiotou, G. Zissopoulou, P. Liatsi. Influence of Temperature and Mobile Phase Composition on Retention Properties of the Macroline Antibiotics Clarithromicin and Roxithromcin in Reversed-phase Liquid Chromatography. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi [Internet]. 2002 Dec. 1;4(2):17-9. Available from: https://izlik.org/JA43GH63MT