Teucrium polium L. demleme ve kaynatma örneklerinin fenolik bileşik ve antioksidan aktivitelerinin araştırılması

Abstract

Bu çalışmada, T. polium' un kaynatma ve demleme örneklerinin fenolik bileşikleri ve antioksidan aktivitesi rapor edilmiştir. Fenolik içeriğin kantitatif miktarları LC-MS/MS ile belirlendi. Kaynatmada belirlenen ana bileşikler ve miktarları sırasıyla; fumarik asit, luteolin-7-O-glikozit, luteolin-5-O-glikozit ve pelargonin (sırasıyla 2060.1; 1167.0; 835.2; 829.9 mg / kg kuru herba) olarak bulundu. Demleme örneği için ise ana bileşikler ve miktarları; fumarik asit, luteolin-7-O-glikozit, pelargonin ve luteolin-5-O-glikozit (sırasıyla 1456.2; 431.1; 312.5; 278.4 mg /kg kuru herba) olarak belirlendi. Antioksidan aktiviteler üç yöntem esas alınarak belirlendi, bunlar: 2,2-difenil-1-pikrilhidrazil (DPPH) serbest radikal süpürme kapasitesi, β-karoten linoleik asit ve kuprik (Cu2+) iyonu indirgeyici antioksidan kapasite (CUPRAC). Tüm analiz sonuçlarına göre, T. polium' un demleme ve kaynatma örnekleri iyi bir aktivite gösterdi.

Keywords

• Teucrium polium,kaynatma,demleme,fenolik bileşik,antioksidan aktivite

Investigation of phenolic compounds and antioxidant activity of Teucrium polium L. decoction and infusion

Abstract

In present study, we report phenolic compounds and antioxidant activity of decoction and infusion of T. polium.  The quantitative amounts of the phenolic contents were determined by LC-MS/MS.  The main compounds and amounts were determined as follow for decoction; fumaric acid, luteolin-7-O-glucoside, luteolin-5-O-glucoside, and pelargonin (2060.1; 1167.0; 835.2; 829.9 mg/kg dried herba, respectively).  For the infusion sample main compounds and amounts were as follow; fumaric acid, luteolin-7-O-glucoside, pelargonin, and luteolin-5-O-glucoside (1456.2; 431.1; 312.5; 278.4 mg/kg dried herba, respectively).  The antioxidant activities were determined based on three methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, β-carotene linoleic acid assays and cupric (Cu²⁺) ion reducing power assay (CUPRAC).  For all the activity assays, infusion and decoction samples of the T. polium showed good activity.

Keywords

• Teucrium polium,decoction,infusion,phenolic compound,antioxidant activity

References

1. Sharififar, F., Dehghn-Nudeh, G. and Mirtajaldini, M., Major flavonoids with antioxidant activity from Teucrium polium L., Food Chemistry, 112, 4, 885-888 (2009).
2. Dinç, M., Dogru, S., Bilgili, B. And Duran A., Comparative anatomical and micromorphological studies on Teucrium creticum and Teucrium orientale var. orientale (T. sect. Teucrium, Lamiaceae), Nordic Journal of Botany, 27, 3, 251-256, (2009).
3. Hasani, P., Yasa, N., Vosough-Ghanbari, S., Mohammadirad, A., Dehghan, G. and Abdollahi, M., In vivo antioxidant potential of Teucrium polium, as compared to α-tocopherol, Acta Pharmaceutica, 57, 1, 123-129, (2007).
4. Bakari, S., Ncir, M., Felhi, S., Hajlaoui, H., Saoudi, M., Gharsallah, N. and Kadri, A., Chemical composition and in vitro evaluation of total phenolic, flavonoid, and antioxidant properties of essential oil and solvent extract from the aerial parts of Teucrium polium grown in Tunisia, Food Science and Biotechnology, 24, 6, 1943-1949, (2015).
5. Sevindik, E., Abacı, Z. T., Yamaner, C. and Ayvaz, M., Determination of the chemical composition and antimicrobial activity of the essential oils of Teucrium polium and Achillea millefolium grown under North Anatolian ecological conditions, Biotechnology & Biotechnological Equipment, 30, 2, 375-380, (2016).
6. Hayta, S., Polat, R. and Selvi, S., Traditional uses of medicinal plants in Elazığ (Turkey), Journal of Ethnopharmacology, 154, 3, 613-623, (2014).
7. Autore, G., Capasso, F., De Fusco, R., Fasulo, M.P., Lembo, M., Mascolo, N. and Menghini, A., Antipyretic and antibacterial actions of Teucrium polium (L.), Pharmacological Research Communications, 16, 1, 21-29, (1984).
8. Gharaibeh, M.N., Elayan, H.H. and Salhab, A.S., Hypoglycemic effects of Teucrium polium, Journal of Ethnopharmacology, 24, 1, 93-99, (1988).

9. Suleiman, M.S., Abdul-Ghani, A.S., Al-Khali, S. and Amin, R., Effect of Teucrium polium boiled leaf extract on intestinal motility and blood pressure, Journal of Ethnopharmacology, 22, 1, 111-116, (1988).
10. Tariq, M., Ageel, A.M., Al-Yahia, M.A., Mossa, J.S. and Al-Said, M.S. Anti-inflammatory activity of Teucrium polium, International Journal of Tissue Reactions, 11, 4, 185-188, (1989).
11. Esmaeili, M.A. and Yazdanparast, R., Hypoglycaemic effect of Teucrium polium: studies with rat pancreatic islets, Journal of Ethnopharmacology, 95, 1, 27-30, (2004).
12. Ljubuncic, P., Dakwar, S., Portnaya, I., Cogan, U., Azaizeh, H. and Bomzon, A., Aqueous extracts of Teucrium polium possess remarkable antioxidant activity in vitro, Evidence-Based Complementary and Alternative Medicine, 3, 3, 329-338, (2006).
13. Rahmouni, F., Hamdaoui, L., Badraoui, R. and Rebai, T., Protective effects of Teucrium polium aqueous extract and ascorbic acid on hematological and some biochemical parameters against carbon tetrachloride (CCl 4) induced toxicity in rats, Biomedicine & Pharmacotherapy, 91, 43-48, (2017).
14. Tepe, B., Degerli, S., Arslan, S., Malatyali, E. and Sarikurkcu, C., Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucrium polium and Stachys iberica, Fitoterapia, 82(2), 237-246, (2011).
15. Rice-Evans, C.A., Miller, N.J. and Paganga, G., Structure-antioxidant activity relationships of flavonoids and phenolic acids, Free Radical Biology & Medicine, 20, 7, 933-956, (1996).
16. Sagir, Z.O., Carikci, S., Kilic, T. and Goren, A.C., Metabolic profile and biological activity of Sideritis brevibracteata PH Davis endemic to Turkey, International Journal of Food Properties, DOI:10.1080/10942912.2016.1265981. Köksal, E., Bursal, E., Gülçin, İ., Korkmaz, M., Çağlayan, C., Gören, A.C. and Alwasel, S.H., Antioxidant activity and polyphenol content of Turkish Thyme (Thymus vulgaris) monitored by LC-MS/MS, International Journal of Food Properties, DOI:10.1080/10942912.2016.1168438.
17. Ertaş, A., Gören, A.C., Haşimi, N., Tolan, V. and Kolak, U., Evaluation of antioxidant, cholinesterase inhibitory and antimicrobial properties of Mentha longifolia subsp. noeana and its secondary metabolites, Records of Natural Products, 9, 1, 105-115, (2015).
18. Blois, M.S., Antioxidant determinations by the use of a stable free radical, Nature, 181, 1199-1200, (1958).
19. Miller, H.E., A simplified method for the evaluation of antioxidants, Journal of American Oil Chemists' Society, 45, 91-98, (1971).
20. Bener, M., Özyürek, M., Güçlü, K. and Apak, R., Optimization of microwave-assisted extraction of curcumin from Curcuma longa L. (turmeric) and evaluation of antioxidant activity in multi-test systems, Records of Natural Products, 10, 5, 794-799, (2016).
21. Bener, M., Shen, Y., Xu, Z. and Apak, R., Changes of the anthocyanins and antioxidant properties of concord Grape (Vitis labrusca) pomace after acid hydrolysis, Records of Natural Products, 10, 6, 542-554, (2016).
22. Özyürek, M., Bener, M., Güçlü, K., Dönmez, A.A., Selçuk, S.S., Pırıldar, S., Meriçli, A.H. and Apak, R., Evaluation of antioxidant activity of Crataegus species collected from different regions of Turkey, Records of Natural Products, 6, 3, 263-277, (2012).
23. Yılmaz, A., Boğa, M. and Topçu, G., Novel terpenoids with potential anti-alzheimer activity from Nepeta obtusicrena, Records of Natural Products, 10, 5, 530-541, (2016).