Teucrium polium L. demleme ve kaynatma örneklerinin fenolik bileşik ve antioksidan aktivitelerinin araştırılması

Öz

Bu çalışmada, T. polium' un kaynatma ve demleme örneklerinin fenolik bileşikleri ve antioksidan aktivitesi rapor edilmiştir. Fenolik içeriğin kantitatif miktarları LC-MS/MS ile belirlendi. Kaynatmada belirlenen ana bileşikler ve miktarları sırasıyla; fumarik asit, luteolin-7-O-glikozit, luteolin-5-O-glikozit ve pelargonin (sırasıyla 2060.1; 1167.0; 835.2; 829.9 mg / kg kuru herba) olarak bulundu. Demleme örneği için ise ana bileşikler ve miktarları; fumarik asit, luteolin-7-O-glikozit, pelargonin ve luteolin-5-O-glikozit (sırasıyla 1456.2; 431.1; 312.5; 278.4 mg /kg kuru herba) olarak belirlendi. Antioksidan aktiviteler üç yöntem esas alınarak belirlendi, bunlar: 2,2-difenil-1-pikrilhidrazil (DPPH) serbest radikal süpürme kapasitesi, β-karoten linoleik asit ve kuprik (Cu2+) iyonu indirgeyici antioksidan kapasite (CUPRAC). Tüm analiz sonuçlarına göre, T. polium' un demleme ve kaynatma örnekleri iyi bir aktivite gösterdi.

Anahtar Kelimeler

• Teucrium polium,kaynatma,demleme,fenolik bileşik,antioksidan aktivite

Investigation of phenolic compounds and antioxidant activity of Teucrium polium L. decoction and infusion

Öz

In present study, we report phenolic compounds and antioxidant activity of decoction and infusion of T. polium.  The quantitative amounts of the phenolic contents were determined by LC-MS/MS.  The main compounds and amounts were determined as follow for decoction; fumaric acid, luteolin-7-O-glucoside, luteolin-5-O-glucoside, and pelargonin (2060.1; 1167.0; 835.2; 829.9 mg/kg dried herba, respectively).  For the infusion sample main compounds and amounts were as follow; fumaric acid, luteolin-7-O-glucoside, pelargonin, and luteolin-5-O-glucoside (1456.2; 431.1; 312.5; 278.4 mg/kg dried herba, respectively).  The antioxidant activities were determined based on three methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, β-carotene linoleic acid assays and cupric (Cu²⁺) ion reducing power assay (CUPRAC).  For all the activity assays, infusion and decoction samples of the T. polium showed good activity.

Anahtar Kelimeler

• Teucrium polium,decoction,infusion,phenolic compound,antioxidant activity

Kaynakça

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