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Antioxidant and anti-proliferative activities of different parts of Cyclamen cilicium

Yıl 2022, , 436 - 447, 08.07.2022
https://doi.org/10.25092/baunfbed.925496

Öz

This study was designed for investigate of antioxidant, and anti-proliferative activities of acetone, methanol and water extracts of different parts (fresh and underground parts) of Cyclamen cilicium extracts. The antioxidant activities were determined using DPPH, ABTS free radical scavenging activities. Anti-proliferative activities of extracts in Caco-2 colon cancer cells were detected by XTT assay. The determination of contents was evaluated using total phenolics, flavonoid and tannin contents and found maximum values 3.69 ± 0.13 (mg GAE/g-extract), 18.48 ± 0.12 (mg QE/g- extract), 41.17 ± 0.44 (mg CE/g- extract) respectively. C. cilicium extracts exhibited anti-proliferative activity in Caco-2 colon cancer cells. IC50 values of methanol and water extracts of fresh part and water extract of underground part were determined as 35.3276 μg/mL, 42.1831 μg/mL and 64.6144 μg/mL at 24h, respectively. The results showed that C. cilicium is the source of natural antioxidants and may have potential use in pharmaceutical, health, biotechnology, agricultural industry.

Destekleyen Kurum

Scientific Research Projects (BAP) of Pamukkale University, Denizli, Turkey as the Doctoral Thesis Project

Proje Numarası

grant number: 2019FEBE001

Kaynakça

  • Guru, A., Lite, C., Freddy, A.J., Issac, P.K., Pasupuleti, M., Saraswathi, N.T., Arasu, M.V., Al-Dhabi, N.A., Arshad, A. and Arockiaraj, J., Intracellular ROS scavenging and antioxidant regulation of WL15 from cysteine and glycine-rich protein 2 demonstrated in zebrafish in vivo model., Developmental and Comparative Immunology, 114, 103863, (2021).
  • Homayouni-Tabrizi, M., Asoodeh, A. and Soltani, M., Cytotoxic and antioxidant capacity of camel milk peptides: effects of isolated peptide on super oxide dismutase and catalase gene expression. Journal of Food and Drug Analysis, 25, 567–575, (2017).
  • Hazra, B., Biswas, S. and Mandal, N., Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complementary and Alternative Medicine, 8, 1–10, (2008).
  • Xu, C., Guan, S., Xu, J., Gong, W., Liu, T., Ma, X. and Sun, C., Preparation, characterization and antioxidant activity of protocatechuic acid grafted carboxymethyl chitosan and its hydrogel. Carbohydrate Polymers, 252, 117210, (2021).
  • Lobo, V., Patil, A., Phatak, A. and Chandra, N., Free radicals, antioxidants and functional foods: impact on human health. Pharmacological Reviews, 4, 118–126, (2010).
  • Byun, H.G., Lee, J.K., Park, H.G., Jeon, J.K. and Kim, S.K., Antioxidant peptides isolated from the marine rotifer, Brachionus rotundiformis. Process Biochemistry, 44, 842–846, (2009).
  • Pereira, J. A., Pereira, A. P., Ferreira, I. C., Valentao, P., Andrade, P. B., Seabra, R., Estevinho, L. and Bento, A., Table olives from Portugal: Phenolic compounds, antioxidant potential, and antimicrobial activity. Journal of Agricultural and Food Chemistry, 54(22), 8425–843, (2006).
  • Dong, R., Liu, S., Zheng, Y., Zhang, X., He, Z., Wang, Z., Wang, Y., Xie, J., Chen, Y. and Yu, Q., Release and metabolism of bound polyphenols from carrot dietary fiber and their potential activity under in vitro digestion and colonic fermentation. Food & Function, 11(7), (2020).
  • Dong, R., Yu, Q., Liao, W., Liu, S., He, Z., Hu, X., Chen, Y., Xie, J., Nie, S. and Xie, M., Composition of bound polyphenols from carrot dietary fiber and its in vivo and in vitro antioxidant activity. Food Chemistry, 339, 127879, (2021).
  • Davis, P.H., Flora of Turkey and East Aegean Islands. Vol. 6. Edinburgh. University of Edinburgh Press, Edinburgh, 128-135, (1978).
  • Güner, A, Aslan, S, Ekim, T, Vural, M and Babaç, M.T., Türkiye Bitkileri Listesi-Damarlı Bitkiler. Nezahat Gökyiğit Vakfı Yayınları, İstanbul, (2012).
  • Çelemli, Ö.G., Kızılpınar Temizer, I. and Altınözlü, H., Anatomy, Palynology of Endemic Cyclamen pseud-ibericum in Mediterranean Phytogeographic Region of Turkey and Chemical Analysis of Its Tuber Extracts. Hacettepe Journal of Biology and Chemistry, 43(2), 105-113, (2015).
  • Prange, A. N. S., Bartsch, M., Serek, M. and Winkelmann, T., Regeneration of different Cyclamen species via somatic embryogenesis from callus, suspension cultures and protoplasts. Scientia Horticulturae, 125, 442–450, (2010).
  • İzgü, T., Sevindik, B., Çürük, P., Şimşek, Ö., Aka Kaçar, Y., Teixeira da Silva, J.A. and Yalçın Mendi, Y., Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey. Plant Cell, Tissue and Organ Culture, 127, 95-113, (2016).
  • Cyclamen cilicium Boiss. & Heldr. in GBIF Secretariat. GBIF Backbone Taxonomy. Checklist dataset. https://www.gbif.org/species/7127370, (2019).
  • Cyclamen cilicium Boiss. & Heldr. in Plant List. The Plant List, Published on the Internet; http://www.theplantlist.org/tpl1.1/record/kew-2749712, (2012). (accessed on 2020-05-07).
  • Yılmaz, U., Kaya, H., Turan, M., Bir, F. and Şahin, B., Investigation the Effect of Hypericum perforatum on Corneal Alkali Burns. Cutaneous and Ocular Toxicology, 38, 356-359, (2019).
  • Wu, C., Chen, F., Wang, X., Kim, H., He, G., Haley-Zitlin, V. and Huang, G., Antioxidant Constituents in Feverfew (Tanacetum parthenium) Extract and Their Chromatographic Quantification. Food Chemistry, 96, 220-227, (2006).
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology & Medicine, 26, 1231-1237, (1999).
  • Singleton, V.L. and Rossi, J.A., Colorimetry of Total Phenolics with Phosphomolybdic-Phoshotungstic Acid Reagents. American Journal of Enology and Viticulture, 16, 144-158, (1965).
  • Aryal, S., Baniya, M.K., Danekhu, K., Kunwar, P., Gurung, R. and Koirala, N., Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants, 8, 96, (2019).
  • Bekir, J., Mars, M., Souchard, J.P. and Bouajila, J., Assessment of Antioxidant, Anti-İnflammatory, Anti-Cholinesterase and Cytotoxic Activities of Pomegranate (Punica granatum) Leaves. Food and Chemical Toxicology, 55, 470-475, (2013).
  • AAT Bioquest, Inc. Quest Graph™ IC50 Calculator.". Retrieved from https://www.aatbio.com/tools/ic50-calculator (accessed on 2021-04-17)
  • Kaska, A., The Phenolic Content, Antioxidant and Cytotoxic Activities of Origanum sipyleum from Turkey. International Journal of Secondary Metabolite, 5, 343-352, (2018).
  • Fernandez-Poytaos, M., Ruiz-Medina, A., Zengin, G. and Llorent-Martinez, E.J., Phenolic Characterization, Antioxidant Activity, and Enzyme Inhibitory Properties of Berberis thunbergii DC. Leaves: A Valuable Source of Phenolic Acids. Molecules, 24, 4171, (2019).
  • Metin, H., Aydın, C., Ozay, C. and Mammadov, R. Antioxidant Activity of the Various Extracts of Cyclamen graecum Link Tubers and Leaves from Turkey, Journal of The Chemical Society of Pakistan, 35(5), 1332-1336, (2013).
  • Turan, M. and Mammadov, R. Antioxidant, Antimicrobial, Cytotoxic, Larvicidal and Anthelmintic Activities and Phenolic Contents of Cyclamen alpinum. Pharmacology & Pharmacy, 9, 100-116, (2018).
  • Zengin, G., Mahomoodally, M.F., Sinan, K.I., Picot-Allain, M.C.N., Yildiztugay, E., Cziaky, Z., Jeko, J., Saleem, H. and Ahemad, N., Chemical characterization, antioxidant, enzyme inhibitory and cytotoxic properties of two geophytes: Crocus pallasii and Cyclamen cilicium. Food Research International, 133, (2020).
  • Sofiane, G. and Wafa, N., Antioxidant, Antimicrobial and Anti-Inflammatory Activities Development of Methanol Extract of Cyclamen africanum B. Et R., Growth in Jijel – Algeria. Journal of Drug Delivery and Therapeutics, 10, 130-134, (2020).
  • Turan, M., Cyclamen alpinum ve Cyclamen parviflorum Ekstraktlarının Fenolik Bileşenleri ve Bazı Biyolojik Özelliklerini Belirlenmesi (in Turkish), Master Thesis, Pamukkale Üniversity, Denizli, Turkey (2016).
  • Stanojević, L.P., Cakić, M.D., Stanojević, J.S., Cvetković, D.J. and Danilović, B.R., Aqueous Extract of Wild Cyclamen Tubers (Cyclamen purpurascens L.) - A Potential Source of Natural Antioxidants and Antimicrobial Agents. Quality of Life, 9(1-2), 13-19, (2018).
  • Kaska, A., Çiçek, M., Deniz, N. and Mammadov, R., Investigation of Phenolic Content, Antioxidant Capacities, Anthelmintic and Cytotoxic Activities of Thymus zygioides Griseb. Journal of Pharmaceutical Research International, 21, 1-13, (2018).
  • Zengin, G. and Aktümsek, A., Investigation of Antioxidant Potentials of Solvent Extracts from Different Anatomical Parts of Asphodeline anatolica E. Tuzlaci: an Endemic Plant to Turkey. African Journal of Traditional, Complementary and Alternative Medicines, 11, 481-488, (2014).
  • Arslan, S., Ozgun, O., Celık, G., Semız, A., Dusen, O., Mammadov, R. and Sen, A., Effects Of Cyclamen trochopteranthum On Hepatic Drug-Metabolizing Enzymes, Archives of Biological Science Belgrade, 63(3), 545-555, (2011).
  • Jaradat, N.A., Al-Masri, M., Hussen, F., Zaid, A.N., Ali, I., Tammam, A., Odeh, D.M., Shakarneh, O.H. and Rajabi, A. Preliminary Phytochemical and Biological Screening of Cyclamen coum a Member of Palestinian Flora, Pharmaceutical Sciences, 23, 231-237, (2017).
  • Speroni, E., Cervellati, R., Costa, S., Dall'Acqua, S., Guerra, M. C., Panizzolo, C., Utan, A., and Innocenti, G., Analgesic and antiinflammatory activity of Cyclamen repandum S. et S. Phytotherapy Research, 21(7), 684–689, (2007). https://doi.org/10.1002/ptr.2145
  • Cornea-Cipcigan M., Pamfil D., Sisea C.R., Gavri C.P., Campos M.G. and Margaoan R., A Review On Cyclamen Species: Transcription Factors vs. Pharmacological Effects. Acta Poloniae Pharmaceutica ñ Drug Research, 76(6), 919-938, (2019).
  • Yildiz, M., Bozcu, H., Tokgun, O., Karagur, E. R., Akyurt, O. and Akca, H., Cyclamen exerts cytotoxicity in solid tumor cell lines: a step toward new anticancer agents? Asian Pacific journal of Cancer Prevention, 14(10), 5911–5913, (2013).
  • Khalilia, W.M., Cytotoxic Activity of Cyclamen Persicum Ethanolic Extract on MCF-7, PC-3 and LNCaP Cancer Cell Lines. Journal of Natural Sciences, 10(2), 42-46, (2020).
  • Karagur, E. R., Ozay, C., Mammadov, R., and Akca, H., Anti-invasive effect of Cyclamen pseudibericum extract on A549 non-small cell lung carcinoma cells via inhibition of ZEB1 mediated by miR-200c. Journal of Natural Medicines, 72(3), 686–693, (2018).
  • Arslan, S., & Ozgun, O. (2012). Cyclamen Trochopteranthum: Cytotoxic activity and possible adverse interactions including drugs and carcinogens. Chinese journal of integrative medicine, Advance online publication. https://doi.org/10.1007/s11655-012-1253-1

Cyclamen cilicium’un farklı kısımlarının antioksidan ve anti-proliferatif aktiviteleri

Yıl 2022, , 436 - 447, 08.07.2022
https://doi.org/10.25092/baunfbed.925496

Öz

Bu çalışma Cyclamen cilicium türünün farklı kısımlarının (toprak üstü ve toprak altı kısımları) aseton, metanol ve su ekstraklarının antioksidan ve anti-proliferatif aktivitelerini araştırılması için tasarlanmıştır. Antioksidan aktivite için DPPH, ABTS serbest radikal giderim aktiviteleri belirlenmiştir. Ekstrelerin Caco-2 kolon kanseri hücrelerindeki anti-proliferatif aktiviteleri XTT testi ile belirlenmiştir. İçeriklerin belirlenmesi için toplam fenolik, flavonoid ve tanen içerikleri belirlenmiştir ve sırasıyla 3.69 ± 0.13 (mg GAE/g-ekstrakt), 18.48 ± 0.12 (mg QE/g-ekstrakt), 41.17 ± 0.44 (mg CE/g-ekstrakt) bulunmuştur. C. cilicium ekstreleri, Caco-2 kolon kanseri hücrelerinde anti-proliferatif aktivite sergilemiştir. Toprak üstü kısmının metanol ve su ekstreleri ve toprak altı kısmının su ekstresinin IC50 değerleri 24 saatte sırasıyla 35.3276 μg/mL, 42.1831 μg/mL and 64.6144 μg/mL bulunmuştur. Sonuçlar, C. cilicium’un doğal antioksidanların bir kaynağı olduğu ve ilaç, sağlık, biyoteknoloji ve tarımsal endüstrilerde potansiyel kullanımlara sahip olabileceğini göstermiştir.

Proje Numarası

grant number: 2019FEBE001

Kaynakça

  • Guru, A., Lite, C., Freddy, A.J., Issac, P.K., Pasupuleti, M., Saraswathi, N.T., Arasu, M.V., Al-Dhabi, N.A., Arshad, A. and Arockiaraj, J., Intracellular ROS scavenging and antioxidant regulation of WL15 from cysteine and glycine-rich protein 2 demonstrated in zebrafish in vivo model., Developmental and Comparative Immunology, 114, 103863, (2021).
  • Homayouni-Tabrizi, M., Asoodeh, A. and Soltani, M., Cytotoxic and antioxidant capacity of camel milk peptides: effects of isolated peptide on super oxide dismutase and catalase gene expression. Journal of Food and Drug Analysis, 25, 567–575, (2017).
  • Hazra, B., Biswas, S. and Mandal, N., Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complementary and Alternative Medicine, 8, 1–10, (2008).
  • Xu, C., Guan, S., Xu, J., Gong, W., Liu, T., Ma, X. and Sun, C., Preparation, characterization and antioxidant activity of protocatechuic acid grafted carboxymethyl chitosan and its hydrogel. Carbohydrate Polymers, 252, 117210, (2021).
  • Lobo, V., Patil, A., Phatak, A. and Chandra, N., Free radicals, antioxidants and functional foods: impact on human health. Pharmacological Reviews, 4, 118–126, (2010).
  • Byun, H.G., Lee, J.K., Park, H.G., Jeon, J.K. and Kim, S.K., Antioxidant peptides isolated from the marine rotifer, Brachionus rotundiformis. Process Biochemistry, 44, 842–846, (2009).
  • Pereira, J. A., Pereira, A. P., Ferreira, I. C., Valentao, P., Andrade, P. B., Seabra, R., Estevinho, L. and Bento, A., Table olives from Portugal: Phenolic compounds, antioxidant potential, and antimicrobial activity. Journal of Agricultural and Food Chemistry, 54(22), 8425–843, (2006).
  • Dong, R., Liu, S., Zheng, Y., Zhang, X., He, Z., Wang, Z., Wang, Y., Xie, J., Chen, Y. and Yu, Q., Release and metabolism of bound polyphenols from carrot dietary fiber and their potential activity under in vitro digestion and colonic fermentation. Food & Function, 11(7), (2020).
  • Dong, R., Yu, Q., Liao, W., Liu, S., He, Z., Hu, X., Chen, Y., Xie, J., Nie, S. and Xie, M., Composition of bound polyphenols from carrot dietary fiber and its in vivo and in vitro antioxidant activity. Food Chemistry, 339, 127879, (2021).
  • Davis, P.H., Flora of Turkey and East Aegean Islands. Vol. 6. Edinburgh. University of Edinburgh Press, Edinburgh, 128-135, (1978).
  • Güner, A, Aslan, S, Ekim, T, Vural, M and Babaç, M.T., Türkiye Bitkileri Listesi-Damarlı Bitkiler. Nezahat Gökyiğit Vakfı Yayınları, İstanbul, (2012).
  • Çelemli, Ö.G., Kızılpınar Temizer, I. and Altınözlü, H., Anatomy, Palynology of Endemic Cyclamen pseud-ibericum in Mediterranean Phytogeographic Region of Turkey and Chemical Analysis of Its Tuber Extracts. Hacettepe Journal of Biology and Chemistry, 43(2), 105-113, (2015).
  • Prange, A. N. S., Bartsch, M., Serek, M. and Winkelmann, T., Regeneration of different Cyclamen species via somatic embryogenesis from callus, suspension cultures and protoplasts. Scientia Horticulturae, 125, 442–450, (2010).
  • İzgü, T., Sevindik, B., Çürük, P., Şimşek, Ö., Aka Kaçar, Y., Teixeira da Silva, J.A. and Yalçın Mendi, Y., Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey. Plant Cell, Tissue and Organ Culture, 127, 95-113, (2016).
  • Cyclamen cilicium Boiss. & Heldr. in GBIF Secretariat. GBIF Backbone Taxonomy. Checklist dataset. https://www.gbif.org/species/7127370, (2019).
  • Cyclamen cilicium Boiss. & Heldr. in Plant List. The Plant List, Published on the Internet; http://www.theplantlist.org/tpl1.1/record/kew-2749712, (2012). (accessed on 2020-05-07).
  • Yılmaz, U., Kaya, H., Turan, M., Bir, F. and Şahin, B., Investigation the Effect of Hypericum perforatum on Corneal Alkali Burns. Cutaneous and Ocular Toxicology, 38, 356-359, (2019).
  • Wu, C., Chen, F., Wang, X., Kim, H., He, G., Haley-Zitlin, V. and Huang, G., Antioxidant Constituents in Feverfew (Tanacetum parthenium) Extract and Their Chromatographic Quantification. Food Chemistry, 96, 220-227, (2006).
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology & Medicine, 26, 1231-1237, (1999).
  • Singleton, V.L. and Rossi, J.A., Colorimetry of Total Phenolics with Phosphomolybdic-Phoshotungstic Acid Reagents. American Journal of Enology and Viticulture, 16, 144-158, (1965).
  • Aryal, S., Baniya, M.K., Danekhu, K., Kunwar, P., Gurung, R. and Koirala, N., Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants, 8, 96, (2019).
  • Bekir, J., Mars, M., Souchard, J.P. and Bouajila, J., Assessment of Antioxidant, Anti-İnflammatory, Anti-Cholinesterase and Cytotoxic Activities of Pomegranate (Punica granatum) Leaves. Food and Chemical Toxicology, 55, 470-475, (2013).
  • AAT Bioquest, Inc. Quest Graph™ IC50 Calculator.". Retrieved from https://www.aatbio.com/tools/ic50-calculator (accessed on 2021-04-17)
  • Kaska, A., The Phenolic Content, Antioxidant and Cytotoxic Activities of Origanum sipyleum from Turkey. International Journal of Secondary Metabolite, 5, 343-352, (2018).
  • Fernandez-Poytaos, M., Ruiz-Medina, A., Zengin, G. and Llorent-Martinez, E.J., Phenolic Characterization, Antioxidant Activity, and Enzyme Inhibitory Properties of Berberis thunbergii DC. Leaves: A Valuable Source of Phenolic Acids. Molecules, 24, 4171, (2019).
  • Metin, H., Aydın, C., Ozay, C. and Mammadov, R. Antioxidant Activity of the Various Extracts of Cyclamen graecum Link Tubers and Leaves from Turkey, Journal of The Chemical Society of Pakistan, 35(5), 1332-1336, (2013).
  • Turan, M. and Mammadov, R. Antioxidant, Antimicrobial, Cytotoxic, Larvicidal and Anthelmintic Activities and Phenolic Contents of Cyclamen alpinum. Pharmacology & Pharmacy, 9, 100-116, (2018).
  • Zengin, G., Mahomoodally, M.F., Sinan, K.I., Picot-Allain, M.C.N., Yildiztugay, E., Cziaky, Z., Jeko, J., Saleem, H. and Ahemad, N., Chemical characterization, antioxidant, enzyme inhibitory and cytotoxic properties of two geophytes: Crocus pallasii and Cyclamen cilicium. Food Research International, 133, (2020).
  • Sofiane, G. and Wafa, N., Antioxidant, Antimicrobial and Anti-Inflammatory Activities Development of Methanol Extract of Cyclamen africanum B. Et R., Growth in Jijel – Algeria. Journal of Drug Delivery and Therapeutics, 10, 130-134, (2020).
  • Turan, M., Cyclamen alpinum ve Cyclamen parviflorum Ekstraktlarının Fenolik Bileşenleri ve Bazı Biyolojik Özelliklerini Belirlenmesi (in Turkish), Master Thesis, Pamukkale Üniversity, Denizli, Turkey (2016).
  • Stanojević, L.P., Cakić, M.D., Stanojević, J.S., Cvetković, D.J. and Danilović, B.R., Aqueous Extract of Wild Cyclamen Tubers (Cyclamen purpurascens L.) - A Potential Source of Natural Antioxidants and Antimicrobial Agents. Quality of Life, 9(1-2), 13-19, (2018).
  • Kaska, A., Çiçek, M., Deniz, N. and Mammadov, R., Investigation of Phenolic Content, Antioxidant Capacities, Anthelmintic and Cytotoxic Activities of Thymus zygioides Griseb. Journal of Pharmaceutical Research International, 21, 1-13, (2018).
  • Zengin, G. and Aktümsek, A., Investigation of Antioxidant Potentials of Solvent Extracts from Different Anatomical Parts of Asphodeline anatolica E. Tuzlaci: an Endemic Plant to Turkey. African Journal of Traditional, Complementary and Alternative Medicines, 11, 481-488, (2014).
  • Arslan, S., Ozgun, O., Celık, G., Semız, A., Dusen, O., Mammadov, R. and Sen, A., Effects Of Cyclamen trochopteranthum On Hepatic Drug-Metabolizing Enzymes, Archives of Biological Science Belgrade, 63(3), 545-555, (2011).
  • Jaradat, N.A., Al-Masri, M., Hussen, F., Zaid, A.N., Ali, I., Tammam, A., Odeh, D.M., Shakarneh, O.H. and Rajabi, A. Preliminary Phytochemical and Biological Screening of Cyclamen coum a Member of Palestinian Flora, Pharmaceutical Sciences, 23, 231-237, (2017).
  • Speroni, E., Cervellati, R., Costa, S., Dall'Acqua, S., Guerra, M. C., Panizzolo, C., Utan, A., and Innocenti, G., Analgesic and antiinflammatory activity of Cyclamen repandum S. et S. Phytotherapy Research, 21(7), 684–689, (2007). https://doi.org/10.1002/ptr.2145
  • Cornea-Cipcigan M., Pamfil D., Sisea C.R., Gavri C.P., Campos M.G. and Margaoan R., A Review On Cyclamen Species: Transcription Factors vs. Pharmacological Effects. Acta Poloniae Pharmaceutica ñ Drug Research, 76(6), 919-938, (2019).
  • Yildiz, M., Bozcu, H., Tokgun, O., Karagur, E. R., Akyurt, O. and Akca, H., Cyclamen exerts cytotoxicity in solid tumor cell lines: a step toward new anticancer agents? Asian Pacific journal of Cancer Prevention, 14(10), 5911–5913, (2013).
  • Khalilia, W.M., Cytotoxic Activity of Cyclamen Persicum Ethanolic Extract on MCF-7, PC-3 and LNCaP Cancer Cell Lines. Journal of Natural Sciences, 10(2), 42-46, (2020).
  • Karagur, E. R., Ozay, C., Mammadov, R., and Akca, H., Anti-invasive effect of Cyclamen pseudibericum extract on A549 non-small cell lung carcinoma cells via inhibition of ZEB1 mediated by miR-200c. Journal of Natural Medicines, 72(3), 686–693, (2018).
  • Arslan, S., & Ozgun, O. (2012). Cyclamen Trochopteranthum: Cytotoxic activity and possible adverse interactions including drugs and carcinogens. Chinese journal of integrative medicine, Advance online publication. https://doi.org/10.1007/s11655-012-1253-1
Toplam 41 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Murat Turan 0000-0003-2900-1755

Mücahit Seçme 0000-0002-2084-760X

Ramazan Mammadov 0000-0003-2218-5336

Proje Numarası grant number: 2019FEBE001
Yayımlanma Tarihi 8 Temmuz 2022
Gönderilme Tarihi 22 Nisan 2021
Yayımlandığı Sayı Yıl 2022

Kaynak Göster

APA Turan, M., Seçme, M., & Mammadov, R. (2022). Antioxidant and anti-proliferative activities of different parts of Cyclamen cilicium. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 24(2), 436-447. https://doi.org/10.25092/baunfbed.925496
AMA Turan M, Seçme M, Mammadov R. Antioxidant and anti-proliferative activities of different parts of Cyclamen cilicium. BAUN Fen. Bil. Enst. Dergisi. Temmuz 2022;24(2):436-447. doi:10.25092/baunfbed.925496
Chicago Turan, Murat, Mücahit Seçme, ve Ramazan Mammadov. “Antioxidant and Anti-Proliferative Activities of Different Parts of Cyclamen Cilicium”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 24, sy. 2 (Temmuz 2022): 436-47. https://doi.org/10.25092/baunfbed.925496.
EndNote Turan M, Seçme M, Mammadov R (01 Temmuz 2022) Antioxidant and anti-proliferative activities of different parts of Cyclamen cilicium. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 24 2 436–447.
IEEE M. Turan, M. Seçme, ve R. Mammadov, “Antioxidant and anti-proliferative activities of different parts of Cyclamen cilicium”, BAUN Fen. Bil. Enst. Dergisi, c. 24, sy. 2, ss. 436–447, 2022, doi: 10.25092/baunfbed.925496.
ISNAD Turan, Murat vd. “Antioxidant and Anti-Proliferative Activities of Different Parts of Cyclamen Cilicium”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 24/2 (Temmuz 2022), 436-447. https://doi.org/10.25092/baunfbed.925496.
JAMA Turan M, Seçme M, Mammadov R. Antioxidant and anti-proliferative activities of different parts of Cyclamen cilicium. BAUN Fen. Bil. Enst. Dergisi. 2022;24:436–447.
MLA Turan, Murat vd. “Antioxidant and Anti-Proliferative Activities of Different Parts of Cyclamen Cilicium”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, c. 24, sy. 2, 2022, ss. 436-47, doi:10.25092/baunfbed.925496.
Vancouver Turan M, Seçme M, Mammadov R. Antioxidant and anti-proliferative activities of different parts of Cyclamen cilicium. BAUN Fen. Bil. Enst. Dergisi. 2022;24(2):436-47.