Cyclamen cilicium’un farklı kısımlarının antioksidan ve anti-proliferatif aktiviteleri

Öz

Bu çalışma Cyclamen cilicium türünün farklı kısımlarının (toprak üstü ve toprak altı kısımları) aseton, metanol ve su ekstraklarının antioksidan ve anti-proliferatif aktivitelerini araştırılması için tasarlanmıştır. Antioksidan aktivite için DPPH, ABTS serbest radikal giderim aktiviteleri belirlenmiştir. Ekstrelerin Caco-2 kolon kanseri hücrelerindeki anti-proliferatif aktiviteleri XTT testi ile belirlenmiştir. İçeriklerin belirlenmesi için toplam fenolik, flavonoid ve tanen içerikleri belirlenmiştir ve sırasıyla 3.69 ± 0.13 (mg GAE/g-ekstrakt), 18.48 ± 0.12 (mg QE/g-ekstrakt), 41.17 ± 0.44 (mg CE/g-ekstrakt) bulunmuştur. C. cilicium ekstreleri, Caco-2 kolon kanseri hücrelerinde anti-proliferatif aktivite sergilemiştir. Toprak üstü kısmının metanol ve su ekstreleri ve toprak altı kısmının su ekstresinin IC50 değerleri 24 saatte sırasıyla 35.3276 μg/mL, 42.1831 μg/mL and 64.6144 μg/mL bulunmuştur. Sonuçlar, C. cilicium’un doğal antioksidanların bir kaynağı olduğu ve ilaç, sağlık, biyoteknoloji ve tarımsal endüstrilerde potansiyel kullanımlara sahip olabileceğini göstermiştir.

Anahtar Kelimeler

• Cyclamen cilicium
• Antioksidan aktivite
• Anti-proliferatif aktivite
• Caco-2 hücreleri
• Kolon kanseri

Antioxidant and anti-proliferative activities of different parts of Cyclamen cilicium

Öz

This study was designed for investigate of antioxidant, and anti-proliferative activities of acetone, methanol and water extracts of different parts (fresh and underground parts) of Cyclamen cilicium extracts. The antioxidant activities were determined using DPPH, ABTS free radical scavenging activities. Anti-proliferative activities of extracts in Caco-2 colon cancer cells were detected by XTT assay. The determination of contents was evaluated using total phenolics, flavonoid and tannin contents and found maximum values 3.69 ± 0.13 (mg GAE/g-extract), 18.48 ± 0.12 (mg QE/g- extract), 41.17 ± 0.44 (mg CE/g- extract) respectively. C. cilicium extracts exhibited anti-proliferative activity in Caco-2 colon cancer cells. IC50 values of methanol and water extracts of fresh part and water extract of underground part were determined as 35.3276 μg/mL, 42.1831 μg/mL and 64.6144 μg/mL at 24h, respectively. The results showed that C. cilicium is the source of natural antioxidants and may have potential use in pharmaceutical, health, biotechnology, agricultural industry.

Anahtar Kelimeler

• Cyclamen cilicium
• Antioxidant activity
• Anti-proliferative activity
• Caco-2 cells
• Colon cancer

Kaynakça

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