Farklı çapraz bağlayıcılar ile hazırlanmış poliakrilamid hidrojellerinin değerlendirilmesi

Yıl 2017, Cilt: 19 Sayı: 2, 177 - 191, 29.09.2017

Sema Ekici , Meziyet Çetik Erkol

https://doi.org/10.25092/baunfbed.340613

Öz

Çapraz
bağlı poliakrilamid (PAAm) hidrojelleri; fiziksel özellikleri üzerine çapraz
bağlayıcı türü etkisinin incelenmesi amacıyla sentezlenmiştir. Bu amaçla;
N,N’-metilenbisakrilamid (MBA), trietilen glikol dimetakrilat (TEGDMA),
glutaraldehit (GLU), divinilsülfon (DVS), N,N’-Diallil L-ltartardiamit (DATD)
ve epiklorhidrin (ECH;) çapraz bağlayıcılar olarak seçilmiş ve bu hidrojeller
sırasıyla PAAm-MBA, PAAm-TEGDMA, PAAm-GLU, PAAm-DVS,
PAAm-DATD, PAAm-ECH olarak isimlendirilmiştir. Tüm çapraz bağlayıcılar
için n_{çapraz bağlayıcı}/n_monomer oranı=%2.5 olarak
sabitlenmiştir. Amonyumpersülfat (APS) ve N,N,N’,N’-tetrametiletilendiamin
(TEMED); redoks başlatıcısı-hızlandırıcısı olarak seçilmiş ve PAAm
hidrojelleri; çapraz bağlayıcıların bulunduğu ortamda monomerlerin radikalik
polimerleşmesi ile hazırlanmıştır. PAAm hidrojellerinin şişme ve
difüzyon parametrelerinin hesaplanabilmesi amacıyla kinetik şişme çalışmaları;
deiyonize suda (DS) ve 25 ^oC’de gerçekleştirilmiştir. Deneysel şişme verileri; şişme prosesinin 2^o’den
kinetiğe uyduğunu göstermiştir. PAAm hidrojellerinin şişme dereceleri;
PAAm-ECH>PAAm-DVS>PAAm-MBA=PAAm-DATD>PAAm-GLU>PAAm-TEGDMA olarak
bulunmuştur. PAAm hidrojelleri; su
moleküllerinin hidrojellere difüzyon türünün Fick tipi olmayan difüzyon türü
olduğunu gösteren ve değeri 0.57 ve 0.80 aralığında değişen sayılara (n)
sahiptir. PAAm
hidrojellerinin çapraz bağlar arası ortalama mol kütlesi (



















), çapraz bağ yoğunluğu (q) ve gözeneklilik (P) gibi ağ yapı
parametreleri hesaplanmıştır. Denge şişme çalışmaları; protein (sığır serumu albumini, BSA),
bakteriyel polisakkarit (dekstran, DEX), vitamin (nikotinamid, NAD) ve ilaç
etken maddesi (8-hidroksi 7-iyodokinolin-5-sülfonik asit, HKSA) çözeltilerinde
de yapılmıştır. Şişme ve ağ yapı parametreleri, PAAm hidrojelleri; yapısı protein, karbohidrat,
vitamin ve ilaç etken moleküllerine benzeyen moleküller ile etkileştirildiğinde
şişme ve adsorpsiyon özelliklerinin tahmin edilmesinde yol gösterici olacaktır. 

Anahtar Kelimeler

Poliakrilamid, çapraz bağlayıcı, hidrojel, şişme derecesi, gözeneklilik

Evaluation of polyacrylamide hydrogels clamped with different crosslinkers

Öz

Crosslinked
polyacrylamide (PAAm) hydrogels were synthesized to investigate the effect of
crosslinker type onto pysical properties of PAAM gels. With this aim; N,N’-methylenebis
acrylamide (MBA), triethylene glycol dimethacrylate
(TEGDMA), glutaraldehyde (GLU),  divinyl
sulfone (DVS), N,N′-diallyl L-tartardiamide (DATD), and epichlorohydrin (ECH)  were selected as crosslinkers and these
hydrogels were called as PAAm-MBA, PAAm-TEGDMA, PAAm-GLU, PAAm-DVS, PAAm-DATD,
and PAAm-ECH, respectively. The ratio of n_crosslinker/n_monomer
was fixed as 2.5% for all crosslinkers. PAAMs were prepared by radical
polymerization of monomers in presence of crosslinkers  and redox initiator-accelerator, i.e., ammonium persulphate (APS) and N,N,N′,N′-tetramethylethylenediamine
(TEMED). Kinetic swelling studies of
hydrogels were carried out in deionize water (DW) at 25 ^◦C for calculating swelling and diffusion parameters of the PAAms. Experimental data of swelling suggest clearly
that the swelling processes obey second-order kinetics. Swelling degrees of the
PAAms were found as PAAm-ECH>PAAm-DVS>PAAm-MBA=PAAm-DATD>PAAm-GLU>PAAm-TEGDMA.
PAAms has numbers (n) between 0.57 and 0.80 indicating
type of diffusion of water molecules to the hydrogels is non-Fickian type. Network
parameters of PAAMs such as average molecular weight between crosslinks (



















), crosslink density (q), and
porosity (P) were calculated. Equilibrium swelling studies were also
realized in solutions of protein (bovine serum albumine, BSA), bacterial
polysaccharide (dextran, DEX), vitamine (nicotineamide, NAD), and drug
(8-hydroxy-7-iodoquinoline-5-sulfonic acid, HKSA). Swelling and network
parameters of the PAAms will be guide to estimate of swelling and adsorption
behaviours of PAAm hydrogels clamped with different crosslinkers when they are
interacted with molecules such as protein, polysaccharide, vitamine, and drug
molecules.

Anahtar Kelimeler

Polyacrylamide, crosslinker, hydrogel, swelling degree, porosity

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