Year 2017, Volume 19 , Issue 2, Pages 192 - 199 2017-09-29

Effects of some thio-crown ethers on human erythrocyte carbonic anhydrase I-II isozymes activities
İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi

Ebru AKKEMİK [1] , Ümit ÇALIŞIR [2] , Baki ÇİÇEK [3]


Carbonic Anhydrases, a member of the class of metalloenzymes, catalyze the conversion of carbon dioxide to bicarbonate and proton. The inhibition or activation of carbonic anhydrases having many isoenzymes is of great physiological importance. That's why we have purified carbonic anhydrase I and II isoenzymes from human erythrocytes. On the activities of the purified enzymes we investigated that effects some thioether (1,10-dithia-18-crown-6 (B1), 1,7-dithia-15-crown-5 (B2), 1,7-dithia-12- (B3), 1,7-dithia-18-crown-6 (B4), 1,10-dithia-21-crown-7 (B5) and 1,4-dithia-12-crown-B6) derivatives in in vitro conditions. B1 and B5 did not show any regular effect on both isoenzyme activity. The best activation effect has been the B2 compound for both isoenzymes.

Metalo enzim sınıfının bir üyesi olan karbonik anhidrazlar karbondioksitin bikarbonata ve protona dönüşümünü katalizlerler. Bir çok izoenzime sahip olan karbonik anhidrazların inhibisyonu veya aktivasyonu fizyolojik açıdan oldukça önemlidir. Bu yüzden bizde çalışmamızda insan eritrositlerinden karbonik anhidraz I ve II izoenzimini saflaştırdık. Saflaştırdığımız enzim aktiviteleri üzerinde bazı tiyo eter türevlerinin (1,10- ditiyo-18-crown-6 (B1), 1,7-ditiyo-15-crown-5 (B2), 1,7- ditiyo -12-crown-4 (B3), 1,7- ditiyo-18-crown-6 (B4), 1,10-ditiyo-21-crown-7 (B5) and 1,4-ditiyo-12-crown-4 (B6)) etkilerini in vitro şartlarda araştırdık. B1 ve B5 her iki izoenzim aktivitesi üzerinde düzenli bir etki göstermezken, en iyi aktivasyon etkisini her iki izoenzim için B2 bileşiği (hCA I için AC50 25.27µM ve hCA II için AC5032.74 µM) göstermiştir.

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Journal Section Article
Authors

Orcid: https://orcid.org/0000-0002-4177-4884
Author: Ebru AKKEMİK

Orcid: https://orcid.org/0000-0001-7699-2008
Author: Ümit ÇALIŞIR

Orcid: https://orcid.org/0000-0003-1257-1188
Author: Baki ÇİÇEK

Dates

Publication Date : September 29, 2017

Bibtex @research article { baunfbed340617, journal = {Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi}, issn = {1301-7985}, eissn = {2536-5142}, address = {Balıkesir Üniversitesi Rektörlük Binası 4.Kat Fen Bilimleri Enstitüsü Çağış / 10145-Balıkesir}, publisher = {Balıkesir University}, year = {2017}, volume = {19}, pages = {192 - 199}, doi = {10.25092/baunfbed.340617}, title = {İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi}, key = {cite}, author = {Akkemi̇k, Ebru and Çalışır, Ümit and Çi̇çek, Baki} }
APA Akkemi̇k, E , Çalışır, Ü , Çi̇çek, B . (2017). İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi . Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi , 19 (2) , 192-199 . DOI: 10.25092/baunfbed.340617
MLA Akkemi̇k, E , Çalışır, Ü , Çi̇çek, B . "İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi" . Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 19 (2017 ): 192-199 <https://dergipark.org.tr/en/pub/baunfbed/issue/31303/340617>
Chicago Akkemi̇k, E , Çalışır, Ü , Çi̇çek, B . "İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi". Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 19 (2017 ): 192-199
RIS TY - JOUR T1 - İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi AU - Ebru Akkemi̇k , Ümit Çalışır , Baki Çi̇çek Y1 - 2017 PY - 2017 N1 - doi: 10.25092/baunfbed.340617 DO - 10.25092/baunfbed.340617 T2 - Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi JF - Journal JO - JOR SP - 192 EP - 199 VL - 19 IS - 2 SN - 1301-7985-2536-5142 M3 - doi: 10.25092/baunfbed.340617 UR - https://doi.org/10.25092/baunfbed.340617 Y2 - 2017 ER -
EndNote %0 Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi %A Ebru Akkemi̇k , Ümit Çalışır , Baki Çi̇çek %T İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi %D 2017 %J Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi %P 1301-7985-2536-5142 %V 19 %N 2 %R doi: 10.25092/baunfbed.340617 %U 10.25092/baunfbed.340617
ISNAD Akkemi̇k, Ebru , Çalışır, Ümit , Çi̇çek, Baki . "İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi". Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 19 / 2 (September 2017): 192-199 . https://doi.org/10.25092/baunfbed.340617
AMA Akkemi̇k E , Çalışır Ü , Çi̇çek B . İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi. BAUN Fen. Bil. Enst. Dergisi. 2017; 19(2): 192-199.
Vancouver Akkemi̇k E , Çalışır Ü , Çi̇çek B . İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2017; 19(2): 192-199.