Effects of some thio-crown ethers on human erythrocyte carbonic anhydrase I-II isozymes activities

Yıl 2017, Cilt: 19 Sayı: 2, 192 - 199, 29.09.2017

Ebru Akkemik , Ümit Çalışır Baki Çiçek

https://doi.org/10.25092/baunfbed.340617

Cited By: 6

Öz

Carbonic Anhydrases, a
member of the class of metalloenzymes, catalyze the conversion of carbon
dioxide to bicarbonate and proton. The inhibition or activation of carbonic
anhydrases having many isoenzymes is of great physiological importance. That's
why we have purified carbonic anhydrase I and II isoenzymes from human
erythrocytes. On the activities of the purified enzymes we investigated that
effects some thioether (1,10-dithia-18-crown-6 (B1), 1,7-dithia-15-crown-5
(B2), 1,7-dithia-12- (B3), 1,7-dithia-18-crown-6 (B4), 1,10-dithia-21-crown-7
(B5) and 1,4-dithia-12-crown-B6) derivatives in in vitro conditions. B1 and B5
did not show any regular effect on both isoenzyme activity. The best activation
effect has been the B2 compound for both isoenzymes.

Anahtar Kelimeler

Carbonic anhydrases, thio crown, activation

İnsan karbonik anhidraz I,II izoenzim aktiviteleri üzerine bazı tiyocrown eterlerin etkisi

Öz

Metalo enzim sınıfının bir üyesi olan karbonik anhidrazlar
karbondioksitin bikarbonata ve protona dönüşümünü katalizlerler. Bir çok
izoenzime sahip olan karbonik anhidrazların inhibisyonu veya aktivasyonu
fizyolojik açıdan oldukça önemlidir. Bu yüzden bizde çalışmamızda insan
eritrositlerinden karbonik anhidraz I ve II izoenzimini saflaştırdık.
Saflaştırdığımız enzim aktiviteleri üzerinde bazı tiyo eter türevlerinin (1,10-
ditiyo-18-crown-6 (B1), 1,7-ditiyo-15-crown-5 (B2), 1,7- ditiyo -12-crown-4
(B3), 1,7- ditiyo-18-crown-6 (B4), 1,10-ditiyo-21-crown-7 (B5) and 1,4-ditiyo-12-crown-4
(B6)) etkilerini in vitro şartlarda araştırdık. B1 ve B5 her iki izoenzim
aktivitesi üzerinde düzenli bir etki göstermezken, en iyi aktivasyon etkisini
her iki izoenzim için B2 bileşiği (hCA I için AC₅₀
25.27µM ve
hCA II için AC₅₀32.74 µM) göstermiştir.

Anahtar Kelimeler

Karbonik anhidraz, tiyocrown, aktivasyon

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