Asemptomatik kullanıcıların kontakt lens saklama kaplarından izole edilen Gram negatif bakterilerin antibiyotik duyarlılıkları

Year 2021, Volume: 23 Issue: 1, 22 - 29, 29.01.2021

Miray Üstüntürk-onan

https://doi.org/10.25092/baunfbed.826100

Abstract

Bu çalışmanın amacı, asemptomatik kullanıcıların kontakt lens saklama kaplarından izole edilen çeşitli bakterilerin in vitro antibiyotik duyarlılıklarını belirlemektir. Bu amaçla yirmi iki bakteri izolatının, Kirby-Bauer disk difüzyon yöntemi kullanılarak in vitro antibiyotik duyarlılıkları araştırılmıştır. Kontrol olarak farklı cinslere ait dört ATCC bakteri suşu kullanılmıştır. Bu çalışmadan elde edilen sonuçlar, test edilen tüm bakterilere karşı gentamisinin en etkili antibiyotik olduğunu göstermiştir. Ek olarak, bu çalışmada test edilen tüm bakterilerin ampisilin, metisilin, penisilin G ve vankomisine karşı dirençli oldukları tespit edilmiştir. Bu sonuçlara göre asemptomatik kullanıcıların kontakt lens saklama kaplarından izole edilen bakterilerin antibiyotik duyarlılıklarının sürekli izlenmesi gerektiği sonucununa varılmıştır.

Keywords

Antibiyotik duyarlılığı, bakteriyel kontaminasyon, kontakt lens saklama kapları

Antibiotic susceptibilities of Gram-negative bacteria isolated from contact lens storage cases of asymptomatic wearers

Abstract

The aim of this study was to determine the in vitro antibiotic susceptibilities of various bacteria isolated from contact lens storage cases of asymptomatic wearers. For this purpose, twenty-two bacteria isolates were subjected to in vitro antibiotic susceptibility using Kirby-Bauer disc diffusion method. Four ATCC strains of different genus of bacteria were used as control. The results obtained from the current study showed that gentamicin was the most effective antibiotic against all bacteria tested. In addition, all bacteria tested in this study were resistant to ampicillin, methicillin, penicillin G and vancomycin. According to the results continuous monitoring of antibiotic susceptibilities of bacteria isolated from contact lens storage cases of asymptomatic wearers are needed.

Keywords

Antibiotic susceptibility, bacterial contamination, contact lens storage cases

References

• Kanpolat, A., Kontakt lensler: Dün, bugün, yarın, Türkiye Klinikleri Journal of Ophthalmology Special Topics, 1, 1-13, (2008).
• Güler, M., Kurt, J., Evren, Ö. and Çeliker, Ü., Yöremizdeki bakteriyel keratitlerin klinik ve mikrobiyolojik özellikleri, Fırat Tıp Dergisi, 13, 235-238, (2008).
• Yünlü, Ö., Özçelik, S. and Arıcı, M. K., Göz kapaklarından ve konjunktivadan alınan sürüntü örneklerinde Acanthamoeba ve diğer serbest yaşayan amiplerin araştırılması, Türkiye Parazitoloji Dergisi, 39, 194-199, (2015).
• Yung, M. S., Boost, M., Cho, P. and Yap, M., Microbial contamination of contact lenses and lens care accessories of soft contact lens wearers (university students) in Hong Kong, Ophthalmic and Physiological Optics, 27, 11-21, (2007).
• Gray, T. B., Cursons, R. T. M., Sherwan, J. F. and Rose, P. R., Acanthamoeba, bacterial, and fungal contamination of contact lens storage cases, British Journal of Ophthalmology, 79, 601–605, (1995).
• Devonshire, P., Munro, F. A., Abernethy, C. and Clark, B. J., Microbial contamination of contact lens cases in the west of Scotland, British Journal of Ophthalmology, 77, 41-45, (1993).
• Larkin, D. F. P., Kilvington, S. and Easty, D. L., Contamination of contact lens storage cases by Acanthamoeba and bacteria, British Journal of Ophthalmology, 74, 133-135, (1990).
• Schaefer, F., Bruttin, O., Zografos, L. and Guex-Crosier, Y., Bacterial keratitis: A prospective clinical and microbiological study, British Journal of Ophthalmology, 85, 842-847, (2001).
• Robertson, D. M., Petroll, W. M., Jester, J. V. and Cavanagh, H. D., Current concepts: Contact lens related Pseudomonas keratitis, Contact Lens and Anterior Eye, 30, 94-107, (2007).
• Alexandrakis, G., Alfonso, E. C. and Miller, D., Shifting trends in bacterial keratitis in south Florida and emerging resistance to fluoroquinolones, Ophthalmology, 107, 1497-502, (2000).
• American Academy of Ophthalmology Cornea/External Disease Panel. Preferred Practice Pattern® Guidelines, Bacterial Keratitis, San Francisco, California: American Academy of Ophthalmology, (2005).
• Chalita, M. R., Hofling-Lima, A. L., Paranhos, A., Schor, P. and Belfort, R., Shifting trends in in vitro antibiotic susceptibilities for common ocular isolates during a period of 15 years, American Journal of Ophthalmology, 137, 43-51, (2004).
• Goldstein, M. H., Kowalski, R. P. and Gardon, Y. J., Emerging fluoroquinolone resistance in bacterial keratitis: a five-year review, Ophthalmology, 106, 1313-1318, (1999).
• Garg, P., Sharma, S. and Rao, G. N., Ciprofloxacin resistant Pseudomonas keratitis, Ophthalmology, 106, 1319-1323, (1999).
• Jensen, H. G. and Felix, C., In vitro susceptibilities of ocular isolates in North and South America, Cornea, 17, 79-87, (1998).
• Pachigolla, G., Blomquist, P. and Cavanagh, H. D., Microbial keratitis pathogens and antibiotic susceptibility: a 5-year review of cases at an urban county hospital in north Texas, Eye and Contact Lens, 33, 45-49, (2007).
• Üstüntürk, M. and Zeybek, Z., Microbial contamination of contact lens storage cases and domestic tap water of contact lens wearers, Wiener Klinische Wochenschrift, 124, 17-22, (2012).
• Barry, A. L. and Thornsberry, C., Susceptibility testing in Lennette EH, Balows A, Hausler WJ, Truant JP, Manual of Clinical Microbiology, 561-574, Washington, District of Columbia: American Society for Microbiology, (1981).
• Clinical and Laboratory Standards Institute, Performance standards for antimicrobial susceptibility testing, Twenty-second informational supplement, (2012).
• Rahim, N., Bano, H. and Naqvi, B., Sensitivity pattern of bacteria isolated from contact lens wearers in the faculty of Pharmacy, Karachi University student population, Iranian Journal of Pharmaceutical Research, 7, 131-134, (2008).
• Jorgensen, J. H. and Ferraro, M. J., Antimicrobial susceptibility testing: a review of general principles and contemporary practices, Clinical Infectious Diseases, 49, 1749–1755, (2009).
• Khan, Z. A., Siddiqui, M. F. and Park, S., Current and emerging methods of antibiotic susceptibility testing, Diagnostics, 9, 49, (2019).
• Çakmaklıoğulları, M. and Çakmaklıoğulları, E. K., Bakteriyel konjonktivit etkenlerinin rutinde kullanılan antibiyotiklere invitro duyarlılıkları, Konuralp Tıp Dergisi, 10, 369-372, (2018).
• Al-Zahrani, S. H. M., Bacteria isolated from contact and non contact lens and antibiotic susceptibility patterns of isolated Pseudomonas aeruginosa, African Journal of Microbiology Research, 6, 7350-7356, (2012).
• Hedayati, H., Ghaderpanah, M., Rasoulinejad, S. A. and Montazeri, M., Clinical presentation and antibiotic susceptibility of contact lens associated microbial keratitis, Journal of Pathogens, 2015, 1-5, (2015).
• Yoon, J. H., Jung, J. W., Moon, H. S., Moon, H. S., Shyn, K. H. and Kim, K. H., Antibiotics susceptibility in bacterial keratitis and proper initial treatment, Journal of Korean Ophthalmological Society, 54, 38-45, (2013).
• Dart, J., The inside story: why contact lens cases become contaminated, Contact Lens and Anterior Eye, 20, 113-118, (1997).
• Szczotka-Flynn, L. B., Imamura, Y., Chandra, J., Yu, C., Mukherjee, P. K., Pearlman, E. and Ghannoum, M. A., Increased resistance of contact lens–related bacterial biofilms to antimicrobial activity of soft contact lens care solutions, Cornea, 28, 918-926, (2009).