Bazı ilaçların koyun Paraoksonaz-1 üzerindeki etkilerinin araştırılması

Yıl 2023, Cilt: 25 Sayı: 2, 483 - 488, 07.07.2023

Hatice Yüksel , Adem Ergün , Mikail Arslan , Oktay Arslan

https://doi.org/10.25092/baunfbed.1205406

Öz

Paraoksonaz-1 (PON1, EC 3.1.8.1) birçok metabolik süreçlerde önemli rollere sahip bir metaloenzimdir. Bu çalışmada Karacabey Merinos koyunundan hidrofobik etkileşim tekniği ile PON1 (mPON1) saflaştırılmıştır. Saf enzim üzerinde enrofloksasin ve furosemid ilaçlarının etkileri araştırılmıştır. Çalışmada kullanılan ilaçların, mPON1 enzimini farklı düzeylerde inhibe ettiği bulunmuştur. İlaçların IC50 değerleri ilgili grafiklerden hesaplanarak inhibisyon güçleri saptanmıştır. Bu değerlere göre furosemid (IC50 = 9,87 mM)’in enrofloksasin (IC50 = 42,21 mM)’den daha güçlü inhibitör olduğu tespit edilmiştir.

Anahtar Kelimeler

Paraoksonaz, inhibisyon, saflaştırma, enrofloksasin, furosemid

Investigation of the effects of some drugs on sheep Paraoxonase-1

Öz

Paraoxonase-1 (PON1, EC 3.1.8.1) is a metalloenzyme that has important roles in many metabolic processes. PON1 (mPON1) was isolated from Karacabey Merino sheep in this study using the hydrophobic interaction method. The effects of enrofloxacin and furosemide on the pure enzyme were investigated. The drugs used in the study were found to inhibit the mPON1 enzyme at various levels. The IC50 values of the drugs were calculated from the related graphics, and their inhibition power was determined. According to these values, furosemide (IC50 = 9.87 mM) was found to be a stronger inhibitor than enrofloxacin (IC50 = 42.21 mM).

Anahtar Kelimeler

Paraoxonase, inhibition, purification, enrofloxacin, furosemide

Kaynakça

• Taler-Verčič, A., Goličnik, M., Bavec A., The Structure and Function of Paraoxonase-1 and Its Comparison to Paraoxonase-2 and-3, Molecules, 25, 1-20, (2020).
• Kulka M., A review of paraoxonase 1 properties and diagnostic applications, Polish Journal of Veterinary Sciences,19, 225–232, (2016).
• Erol, K., Gençer, N., Arslan, M. Arslan, O., Purification, characterization, and investigation of in vitro inhibition by metals of paraoxonase from different sheep breeds, Artificial Cells Nanomedicine and Biotechnology, 41, 125-130, (2013).
• Koncuk Cebeci, B., Alim, Z., Beydemir, Ş., In vitro effects of pesticide exposure on the activity of the paraoxonase-1 enzyme from sheep liver microsomes, Turkish Journal of Chemistry, 38 ,512-20, (2014).
• Sayin, D., Çakir, D.T., Gençer, N., Arslan, O., Effects of some metals on paraoxonase activity from shark Scyliorhinus canicula, Journal of Enzyme Inhibition and Medicinal Chemistry., 27, 595-598, (2012).
• Ergün, A., Effects of Some Pesticides on Purified Human Paraoxonase 1 Activity, In Vitro, Fresenius Environmental Bulletin, 31, 9627-9633, (2022).
• Cao, Y., Zhang, J., Yang, W., Xia, C., Zhang, H. Y., Wang, Y. H., C. Xu, Serum paraoxonase as an indicator for fatty liver in sheep, Journal of Veterinary Research, 61, 97-102, (2017).
• Tas, E. C., Filipuci, I., Cakir, D. T., Beyaztas, S., Sunlu, U., Togulga, M., Ozaydin, O., Arslan, O. Heavy metal concentrations in tissues of edible fish (Mullus Barbatus L., 1758) from Candarlı Bay ( Turkey), Fresenius Environmental Bulletin, 20, 2834-2839, (2011).
• Ceron, J.J., Tecles, F., Tvarijonaviciute, A., Serum paraoxonase 1 (PON1) measurement: An update, BMC Veterinary Research, 10, (2014).
• Rincón, J. Madeira, E.M., Campos, F.T., Mion, B,. Silva, J.F. V.A., Absalón-Medina, W.R. Butler, M.N. Corrêa, L. Pegoraro, A. Schneider, Exogenous paraoxonase-1 during oocyte maturation improves bovine embryo development in vitro, Reproduction in Domestic Animals, 51, 827-830, (2016).
• Pradieé, J., de Campos, F.T., Rincon, J.A.A., Collares, L., Goularte, K., Silveira, P.A.S., Pegoraro, L.M.C., A. Schneider, Paraoxonase 1 (PON1) activity in serum, follicular fluid and seminal plasma of sheep, Reproduction in Domestic Animals, 52, 1142-1144, (2017).
• Arab, Z.N., Khayatan, D., Razavi, S.M., Zare, K., Kheradkhah, E., Momtaz, S., Ferretti, G., Bacchetti, T., Sathyapalan, T., Emami, S.A., Abdolghaffari, A.H., Sahebkar, A., Phytochemicals as Modulators of Paraoxonase‐1 in Health and Diseases, Antioxidants, 11, 1-25, (2022).
• Nadaroglu, H., Kucukoglu, K., Koc, F., Investigation of in vivo effect of florfenicol on metabolic-antioxidant enzymes’ activities on Morkaraman normal and lactating sheep, Journal of Taibah University for Science, 7, 189-194, (2013).
• Sinan, S., Kockar, F., Arslan, O., Novel purification strategy for human PON1 and inhibition of the activity by cephalosporin and aminoglikozide derived antibiotics, Biochimie, 88, 565-574, (2006).
• Arslan, M., Erzengin, M., Demir, D,, Comparison of serum paraoxonase 1 (PON1) activities among different sheep breeds in Turkey, Journal of Animal and Veterinary Advances., 10, 489-494, (2011).
• Türkeş, C., Beydemir, Ş., Küfrevioğlu, Ö.İ., In Vitro and In Silico Studies on the Toxic Effects of Antibacterial Drugs as Human Serum Paraoxonase 1 Inhibitor, ChemistrySelect, 4, 9731-9736, (2019).
• Türkeş, C., Beydemir, Ş., Inhibition of Human Serum Paraoxonase-I with Antimycotic Drugs: In Vitro and In Silico Studies, Applied Biochemistry and Biotechnology, 190, 252-269, (2020).
• Karataş, M.O., Çalgın, G., Alıcı, B., Gökçe, B., Gençer, N., Taşkın Tok T., Arslan, O., Kılıç-Cıkla, I., Özdemir, N., Inhibition of paraoxonase 1 by coumarin-substituted N-heterocyclic carbene silver(I), ruthenium(II) and palladium(II) complexes, Applied Organometallic Chemistry, 33 (2019).
• Manna, S., Sharma, A., Satpati, A.K., Electrochemical methods in understanding the redox processes of drugs and biomolecules and their sensing, Current Opinion in Electrochemistry, 32, 100886, (2022).
• Yamasaki, K., Chuang, V.T.G., Maruyama, T., Otagiri, M., Albumin-drug interaction and its clinical implication, Biochimica et Biophysica Acta - General Subjects, 1830, 5435–5443, (2013).
• Paul, B.K., Ghosh, N., Mukherjee, S., Binding interaction of a prospective chemotherapeutic antibacterial drug with β-lactoglobulin: Results and challenges, Langmuir, 30, 5921-5929, (2014).