Bazı ilaçların koyun Paraoksonaz-1 üzerindeki etkilerinin araştırılması

Öz

Paraoksonaz-1 (PON1, EC 3.1.8.1) birçok metabolik süreçlerde önemli rollere sahip bir metaloenzimdir. Bu çalışmada Karacabey Merinos koyunundan hidrofobik etkileşim tekniği ile PON1 (mPON1) saflaştırılmıştır. Saf enzim üzerinde enrofloksasin ve furosemid ilaçlarının etkileri araştırılmıştır. Çalışmada kullanılan ilaçların, mPON1 enzimini farklı düzeylerde inhibe ettiği bulunmuştur. İlaçların IC50 değerleri ilgili grafiklerden hesaplanarak inhibisyon güçleri saptanmıştır. Bu değerlere göre furosemid (IC50 = 9,87 mM)’in enrofloksasin (IC50 = 42,21 mM)’den daha güçlü inhibitör olduğu tespit edilmiştir.

Anahtar Kelimeler

• Paraoksonaz
• inhibisyon
• saflaştırma
• enrofloksasin
• furosemid

Investigation of the effects of some drugs on sheep Paraoxonase-1

Öz

Paraoxonase-1 (PON1, EC 3.1.8.1) is a metalloenzyme that has important roles in many metabolic processes. PON1 (mPON1) was isolated from Karacabey Merino sheep in this study using the hydrophobic interaction method. The effects of enrofloxacin and furosemide on the pure enzyme were investigated. The drugs used in the study were found to inhibit the mPON1 enzyme at various levels. The IC50 values of the drugs were calculated from the related graphics, and their inhibition power was determined. According to these values, furosemide (IC50 = 9.87 mM) was found to be a stronger inhibitor than enrofloxacin (IC50 = 42.21 mM).

Anahtar Kelimeler

• Paraoxonase
• inhibition
• purification
• enrofloxacin
• furosemide

Kaynakça

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