Ornithogalum nutans L. Metanol ekstraktlarının antioksidan, enzim inhibitör Etkileri, total sekonder metabolit miktar tayininin araştırılması

Yıl 2024, Cilt: 26 Sayı: 1, 305 - 315, 19.01.2024

Mehmet Özgür Atay , Gülsüm Cengaver , Yağmur Güzel , Ramazan Mammadov

https://doi.org/10.25092/baunfbed.1242438

Öz

Bu çalışma Ornithogalum nutans türünün farklı kısımlarının (yerüstü ve yeraltı) metanol ekstraktlarının antioksidan ve enzim inhibitör aktivitelerinin belirlenmesinin yanı sıra total fenolik ve flavonoid miktarlarının araştırılması için tasarlanmıştır. Ekstraktların total fenolik miktarı 1.43±0.01/3.05±0.04 mg GAE/g ekstrakt eşdeğeri arasında değişirken, total flavonoid miktarı ise 0.33±0.01/1.79±0.02 mg QE/g arasında değişmektedir. Antioksidan aktiviteleri DPPH, ABTS, ß-Karoten/Linoleik asit, CUPRAC ve FRAP yöntemleri ile belirlemiştir. Yapılan antioksidan aktivite çalışmalarında yerüstü metanol ekstraktının, yeraltına göre daha yüksek bir aktivite sergilediği tespit edilmiştir. Ekstraktların asetilkolinesteraz ve tirozinaz enzim inhibitör aktiviteleri belirlenmiştir ve ekstraktların standart olarak kullanılan galantamin (89.41±0.05 %) ve kojik asite (89.41±0.05 %) göre daha düşük bir aktivite sergilemişlerdir. Sonuçlar, O. nutans ekstraktlarının farmakolojik çalışmalarda ilaç ve gıda endüstrilerinde kullanılabilme potansiyellerini ortaya koymaktadır.

Anahtar Kelimeler

Ornithogalum nutans, antioksidan, asetilkolinesteraz, tirozinaz, sekonder metabolit

Proje Numarası

1919B012102441

Investigation of antioxidant, enzyme inhibitory effects and total secondary metabolite quantification of Ornithogalum nutans L. methanol

Öz

This study was designed to determine the antioxidant and enzyme inhibitory activities of methanol extracts of different parts (aerial and bulb) of Ornithogalum nutans as well as to investigate the total phenolic and flavonoid amounts. While the total phenolic content of the extracts varied between 1.43±0.01/3.05±0.04 mg GAE/g of extract equivalent, the total flavonoid amount varied between 0.33±0.01/1.79±0.02 mg QE/g. Antioxidant activities were determined by DPPH, ABTS, ß-Carotene/Linoleic acid, CUPRAC, and FRAP methods. The antioxidant activity studies determined that the aerial methanol extract exhibited a higher activity than the bulb. Acetylcholinesterase and tyrosinase enzyme inhibitory activities of the extracts were determined, and the extracts showed lower activity than galantamine (89.41±0.05 %) and kojic acid (89.41±0.05 %) used as standard. The results reveal the potential of O. nutans extracts to be used in pharmacological studies in the pharmaceutical and food industries.

Anahtar Kelimeler

Ornithogalum nutans, antioxidant, acetylcholinesterase, tyrosinase, secondary metabolites

Destekleyen Kurum

TUBİTAK

Proje Numarası

1919B012102441

Kaynakça

• Tavşanoğlu, Ç., Yokeş, M. B., Soyumert, A., İpekdal, K., Sağlam, İ. K., Yücel, M. And Dalfes H. N., Turkey's globally important biodiversity in crisis, Biological Conservation, 144(12), 2752-2769, (2011).
• Gross, M., Turkey's biodiversity at the crossroads, Current Biology, 22(13), R503-R505, (2012).
• Demir, A., Sürdürülebilir gelişmede yükselen değer; biyolojik çeşitlilik açısından Türkiye değerlendirmesi, İstanbul Ticaret Üniversitesi Fen Bilimleri Dergisi, 12(24), 67-74, (2013).
• Wachtel-Galor, S., and Benzie, I. F., Herbal Medicine. CRC Press, 1, New York, (2011).
• Plančić, M., Božin, B., Kladar, N., Rat, M., and Srđenović, B., Phytochemical profile and biological activities of the genus Ornithogalum L. (Hyacinthaceae), Biologia Serbica, 36 (1-2), 3-17, 2015.
• Güven, A., and Gürsul, I., Bitki doku kültürlerinde sekonder metabolit sentezi, Gıda, 39(5), 299-306, (2014).
• Tiring, G., Satar, S., and Özkaya, O., Sekonder Metabolitler, Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 35(1), 203-215, (2020).
• Aydın, Ç., Özay, C., and Mammadov, R., Türkiye’de yayılış gösteren Cyclamen L. türleri üzerinde yapılan çalışmalar. Hacettepe University Journal of Faculty of Pharmcy, 34(2), 96-112, (2014).
• Dumanović, J., Nepovimova, E., Natić, M., Kuča, K., and Jaćević, V., The significance of reactive oxygen species and antioxidant defense system in plants: a concise overview, Frontiers in Plant Science, 11, 552969, (2021).
• Sargın, S., Selvi, S., Akçiçek, E., Alaşehir (Manisa) ve çevresinde yetişen bazı geofitlerin etnobotanik açıdan incelenmesi, Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi, 29 (2), 170-178, (2013).
• Uysal, T., Ertuğrul, K., and Dural, H., A new species of Ornithogalum (Liliaceae) from south Anatolia, Turkey, Botanical Journal of the Linnean Society, 148(4), 501-504, (2005).
• Meriç, Ç., Aksoy, Ö., and Dane, F., Morphological and anatomical contributions to the taxonomical identification of two Ornithogalum taxa (O. nutans and O. boucheanum) from Flora of Turkey, Biologia, 66(1), 68-75, (2011).
• Yılmaz, G., Seed micromorphology of Ornithogalum refractum and Ornithogalum fimbriatum Hyacinthaceae from Turkey, Biyolojik Çeşitlilik ve Koruma. 7(2), 110-114, (2014).
• Atay, M. Ö., Ardıl, B., Alper, M., and Ceylan, O., Determination of toxic and anthelmintic activities of Ornithogalum nutans L., Sternbergia lutea (L.) Ker-Gawl. ex Spreng. and Allium stylosum O. Schwarz, Natural Products and Biotechnology, 1(1), 38-48, (2021).
• Turan, M., and Mammadov, R., Antioxidant, antimicrobial, cytotoxic, larvicidal and anthelmintic activities and phenolic contents of Cyclamen alpinum, Pharmacology & Pharmacy, 9(04), 100, (2018).
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C., Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radical Biology and Medicine, 26(9-10), 1231-1237, (1999).
• Amarowicz, R., Pegg, R. B., Rahimi-Moghaddam, P., Barl, B., and Weil, J. A., Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies, Food Chemistry, 84(4), 551-562, (2004).
• Apak, R., Güçlü, K., Özyürek, M., and Karademir, S. E., Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method, Journal of Agricultural and Food Chemistry, 52(26), 7970-7981 (2004).
• Benzie, I. F., and Strain, J. J., The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay, Analytical Biochemistry, 239(1), 70-76, (1996).
• Ellman, G. L., Courtney, K. D., Andres Jr, V., and Featherstone, R. M., A new and rapid colorimetric determination of acetylcholinesterase activity, Biochemical Pharmacology, 7(2), 88-95, (1961).
• Sharaf, O. Z. and Orhan, M. F., An overview of fuel cell technology: Fundamentals and application, Renewable and Sustainable Energy Reviews, 32, 810-853, (2014).
• Singleton, V. L., and Rossi, J. A., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, American Journal of Enology and Viticulture, 16(3), 144-158, (1965).
• Aryal, B., and Suárez, Y., Non-coding RNA regulation of endothelial and macrophage functions during atherosclerosis, Vascular Pharmacology, 114, 64-75, (2019).
• Rat, M. M., Gavaric, N. S., Kladar, N. V., Andric, A. M., Anackov, G. T., and Bozin, B. N., The phenolics of the Ornithogalum umbellatum L. (Hyacinthaceae): phytochemical and ecological characterization, Chemistry & Biodiversity, 13(11), 1551-1558, (2016).
• Temiz, M. A. Investigation of phenolic composition, antioxidant capacity, and antidiabetic effect of Ornithogalum lanceolatum L.: An in vitro study, International Journal of Secondary Metabolite, 8(2), 94-103, (2021).
• Aydin, S., Total phenolic content, antioxidant, antibacterial and antifungal activities, FT-IR analyses of Brassica oleracea L. var. acephala and Ornithogalum umbellatum L, Genetika, 52(1), 229-244, (2020).
• Renda, G., Özel, A., Turumtay, E. A., Barut, B., Korkmaz, B., Ayvaz, M. Ç., and Demir, A., Comparison of phenolic profiles and antioxidant activity of three Ornithogalum L. species, Turkish Journal of Biochemistry, 44(3), 299-306, (2019).
• Ebrahimzadeh, M. A., Nabavl, S. M., Nabavl, S. F., and Eslami, B., Antioxidant activity of the bulb and aerial parts of Ornithogalum sintenisii L (Liliaceae) at flowering stage, Tropical Journal of Pharmaceutical Research, 9(2), 141-148, (2010).
• Zengin, G., Uysal, S., Ceylan, R., and Aktumsek, A., Phenolic constituent, antioxidative and tyrosinase inhibitory activity of Ornithogalum narbonense L. from Turkey: A phytochemical study, Industrial Crops and Products, 70, 1-6, (2015).
• Kurt, B. Z., Gazioğlu, I., Sevgi, E., and Sönmez, F., Anticholinesterase, antioxidant, antiaflatoxigenic activities of ten edible wild plants from Ordu area, Turkey, Iranian Journal of Pharmaceutical Research: IJPR, 17(3), 1047, (2018).
• Makasçı, A. A., Mammadov, R., Düşen, O., and Işık, H., Antimicrobial and antioxidant activities of medicinal plant species Ornithogalum alpigenum stapf. from Turkey, Journal of Medicinal Plants Research, 4(16), 1637-1642, (2010).
• Taşkın, D., Şentürk, Ş., Güler, E. M., Doğan, A., Omurtag, G. Z., Ermanoğlu, M., and Taşkın, T., Biological activities of the Ornithogalum orthophyllum and its in silico ADMET profile. Traditional Medicine Research, 6(6), (2021).
• Gönel, A., Akdağ, A., and Yilmaz, M. A., Identification of phenolic compounds, antioxidant activity and anti-cancer effects of the extract obtained from the shoots of Ornithogalum narbonense L., Cellular and Molecular Biology, 64(1), 75-83, (2018).
• Lim, T.Y., Lim, Y.Y., and Yule, C.M., Evaluation of antioxidant, antibacterial and anti-tyrosinase activities of four Macaranga species, Food Chemistry, 114 (2), 594–599, (2009).
• Delazar, A., Nazifi, E., Movafeghi, A., Nazemiyeh, H., Hemmati, S., Nahar, L., and Sarker, S. D., Analyses of phytosterols and free radical scavengers in the bulbs of Ornithogalum cuspidatum Bertol, Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 9(2), 87-92, (2010).
• Stankovic, M.S., Total phenolic content, flavonoid concentration, and antioxidant activity of Marrubium peregrinum L. extracts, Kragujevac Journal of Sciences, 33, 63-72, (2011).
• Baba, S. A., and Malik, S. A., Determination of total phenolic and flavonoid content, the antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume, Journal of Taibah University for Science, 9(4), 449-454, (2015).