Purification and characterization of polyphenol oxidase enzyme from Iğdır apricot (Prunus armeniaca L.)

Volume: 2 Number: 1 June 23, 2012
  • Fatih Çağlar Çelikezen
  • Halit Demir
  • Çilem Çimen
EN

Purification and characterization of polyphenol oxidase enzyme from Iğdır apricot (Prunus armeniaca L.)

Abstract

In this study, polyphenol oxidase (E.C. 1.14.18.1; PPO) enzyme obtained from Igdir Apricot was purified with method of affinity chromatography. The apricot cultivar “Salak ” was provided from Igdir region. To purify polyphenol oxidase enzyme obtained

Keywords

References

  1. Anosike EO, Ojimelukwe PC (1982). Partial purification and characterizatin of polyphenol oxidase from cocoyam, Xanthosoma sagitifolium. J Exp Bot 33, 487- 494.
  2. Aydemir T (2004). Partial purification and characteriza- tion of polyphenol oxidase from artichoke (Cynara scolymus L.). Food Chem 87, 59–67.
  3. Balasingam K, Ferdinand W (1997). The purification and properties of a ribonucleo enzyme, o-diphenol oxidase, from patotoes. Biochem J 118, 15-23.
  4. Ben-Shalom N, KahnV, Harl E, Mayer AW (1997). Catechol oxidase from green olives: properties and partial purification. Phytochem 16, 1153-1158.
  5. Bradford MA (1976). Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254.
  6. Chevalier T, Rigal D, Mbe´guie´-A-Mbe´guie´ D, Gauillard F, Richard-Forget F, Fils-Lycaon B R (1999). Molecular cloning and characterization of apricot fruit poly- phenol oxidase. Plant Phy 119, 1261-1269.
  7. Coseteng MY, Lee CY (1978). Changes in apple poly- phenol oxidase and polyphenol concentrations in relation to degree of browning. J Food Sci 52, 985-989.
  8. Cuatrecases P (1970). Protein purification by affinity chromotography. J Biochem 245, 3059-3065.

Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Fatih Çağlar Çelikezen This is me

Halit Demir This is me

Çilem Çimen This is me

Publication Date

June 23, 2012

Submission Date

January 16, 2012

Acceptance Date

March 16, 2012

Published in Issue

Year 2012 Volume: 2 Number: 1

APA
Çelikezen, F. Ç., Demir, H., & Çimen, Ç. (2012). Purification and characterization of polyphenol oxidase enzyme from Iğdır apricot (Prunus armeniaca L.). Bitlis Eren University Journal of Science and Technology, 2(1), 22-26. https://doi.org/10.17678/beuscitech.47148
AMA
1.Çelikezen FÇ, Demir H, Çimen Ç. Purification and characterization of polyphenol oxidase enzyme from Iğdır apricot (Prunus armeniaca L.). Bitlis Eren University Journal of Science and Technology. 2012;2(1):22-26. doi:10.17678/beuscitech.47148
Chicago
Çelikezen, Fatih Çağlar, Halit Demir, and Çilem Çimen. 2012. “Purification and Characterization of Polyphenol Oxidase Enzyme from Iğdır Apricot (Prunus Armeniaca L.)”. Bitlis Eren University Journal of Science and Technology 2 (1): 22-26. https://doi.org/10.17678/beuscitech.47148.
EndNote
Çelikezen FÇ, Demir H, Çimen Ç (June 1, 2012) Purification and characterization of polyphenol oxidase enzyme from Iğdır apricot (Prunus armeniaca L.). Bitlis Eren University Journal of Science and Technology 2 1 22–26.
IEEE
[1]F. Ç. Çelikezen, H. Demir, and Ç. Çimen, “Purification and characterization of polyphenol oxidase enzyme from Iğdır apricot (Prunus armeniaca L.)”, Bitlis Eren University Journal of Science and Technology, vol. 2, no. 1, pp. 22–26, June 2012, doi: 10.17678/beuscitech.47148.
ISNAD
Çelikezen, Fatih Çağlar - Demir, Halit - Çimen, Çilem. “Purification and Characterization of Polyphenol Oxidase Enzyme from Iğdır Apricot (Prunus Armeniaca L.)”. Bitlis Eren University Journal of Science and Technology 2/1 (June 1, 2012): 22-26. https://doi.org/10.17678/beuscitech.47148.
JAMA
1.Çelikezen FÇ, Demir H, Çimen Ç. Purification and characterization of polyphenol oxidase enzyme from Iğdır apricot (Prunus armeniaca L.). Bitlis Eren University Journal of Science and Technology. 2012;2:22–26.
MLA
Çelikezen, Fatih Çağlar, et al. “Purification and Characterization of Polyphenol Oxidase Enzyme from Iğdır Apricot (Prunus Armeniaca L.)”. Bitlis Eren University Journal of Science and Technology, vol. 2, no. 1, June 2012, pp. 22-26, doi:10.17678/beuscitech.47148.
Vancouver
1.Fatih Çağlar Çelikezen, Halit Demir, Çilem Çimen. Purification and characterization of polyphenol oxidase enzyme from Iğdır apricot (Prunus armeniaca L.). Bitlis Eren University Journal of Science and Technology. 2012 Jun. 1;2(1):22-6. doi:10.17678/beuscitech.47148

Cited By