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Purification and characterization of polyphenol oxidase enzyme from Iğdır apricot (Prunus armeniaca L.)

Year 2012, Volume: 2 Issue: 1, 22 - 26, 23.06.2012
https://doi.org/10.17678/beuscitech.47148

Abstract

In this study, polyphenol oxidase (E.C. 1.14.18.1; PPO) enzyme obtained from Igdir Apricot was purified with method of affinity chromatography. The apricot cultivar “Salak ” was provided from Igdir region. To purify polyphenol oxidase enzyme obtained

References

  • Anosike EO, Ojimelukwe PC (1982). Partial purification and characterizatin of polyphenol oxidase from cocoyam, Xanthosoma sagitifolium. J Exp Bot 33, 487- 494.
  • Aydemir T (2004). Partial purification and characteriza- tion of polyphenol oxidase from artichoke (Cynara scolymus L.). Food Chem 87, 59–67.
  • Balasingam K, Ferdinand W (1997). The purification and properties of a ribonucleo enzyme, o-diphenol oxidase, from patotoes. Biochem J 118, 15-23.
  • Ben-Shalom N, KahnV, Harl E, Mayer AW (1997). Catechol oxidase from green olives: properties and partial purification. Phytochem 16, 1153-1158.
  • Bradford MA (1976). Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254.
  • Chevalier T, Rigal D, Mbe´guie´-A-Mbe´guie´ D, Gauillard F, Richard-Forget F, Fils-Lycaon B R (1999). Molecular cloning and characterization of apricot fruit poly- phenol oxidase. Plant Phy 119, 1261-1269.
  • Coseteng MY, Lee CY (1978). Changes in apple poly- phenol oxidase and polyphenol concentrations in relation to degree of browning. J Food Sci 52, 985-989.
  • Cuatrecases P (1970). Protein purification by affinity chromotography. J Biochem 245, 3059-3065.
  • Demir H (2004). Purification and characterization of polyphenol oxidase from Erciş apple. Bull Pure Appl Sci C-Chem 23, 15-20.
  • Demir H, Yörük İH, Savran A, Ekici K (2006). Prufication and properties of polyphenol oxidase from Van apple (Golden delicious). Asian J Chem 18, 475-480.
  • Doğan S, Turan Y, Ertürk H, Arslan O (2005). Characterization and purification of polyphenol oxidase from artichoke (Cynara scolymus L.). J Agric Food Chem 53, 776-785.
  • Erat M, Sakiroglu H, Kufrevioglu OK (2006). Purification and characterization of polyphenol oxidase from Ferula sp. Food Chem 95, 503–508.
  • Jharna RD, Santhoor GB, Lalitha RG (1997). Purification and characterization of a polyphenol oxidase from the kew cultivar of Indian pineapple fruit. J Agric Food Chem 45, 2031-2035.
  • Laemmli DK (1970). Cleavage of structural proteins during in asembly of the head of bacteriophage T4. Nature 227, 680-685.
  • Mathew AG, Parpia HAB (1971). Food browning as a polyphenol reaction. Adv Food Res 19, 75-145.
  • Palmer JK (1963). Banana polyphenoxidase prepartion and properties. Phy Plant 38, 508- 513.
  • Schaller K (1972). Zur bestimmung der polyphenol- oksidase aktivat in kartoffelknollen. Z Lebensm Unters Forsch 150, 211-216.
  • Segel IH (1968). Biochemical calculations, second edition, Publised by, John Wiley and Sons Inc. New York, USA, 458 pp., ISBN: 0-471-77421-9.
  • Ünal MÜ (2007). Properties of polyphenol oxidase from Anamur banana (Musa cavendishii). Food Chem 100, 909-913.
  • Wildanger W, Herrmann K (1973). Die phenolicshen inhaltsstofee des obstes. H. die flovonole des obstes. Z Lebensm Unters Forsch 151, 103-108.
  • Valero E, Varon R, Garcia-Carmona F (1988). Characterization of polyphenol oxidase from airen grapes. J Food Sci 53, 1482-1485.
  • Vamos-Vigyazo L (1981). Polyphenol oxidase and peroxidase in fruits and vegetables. Crit Rev Food Sci Nutr 15, 49-127.
  • Yerlitürk FÜ (2003). Peyrus elaegrifolia meyvesinden polifenol oksidaz enziminin afinite kromatogtrafisi ile saflaştırılması incelenemesi. Science Institue, Master Thesis, Balıkesir University, 53 pp. kinetik özelliklerinin
  • Ziyan E, Pekyardımcı S (2004). Purification and characterization of pear (Pyrus communis) polyphenol oxidase. Turk J Chem 28, 547-557.
Year 2012, Volume: 2 Issue: 1, 22 - 26, 23.06.2012
https://doi.org/10.17678/beuscitech.47148

Abstract

References

  • Anosike EO, Ojimelukwe PC (1982). Partial purification and characterizatin of polyphenol oxidase from cocoyam, Xanthosoma sagitifolium. J Exp Bot 33, 487- 494.
  • Aydemir T (2004). Partial purification and characteriza- tion of polyphenol oxidase from artichoke (Cynara scolymus L.). Food Chem 87, 59–67.
  • Balasingam K, Ferdinand W (1997). The purification and properties of a ribonucleo enzyme, o-diphenol oxidase, from patotoes. Biochem J 118, 15-23.
  • Ben-Shalom N, KahnV, Harl E, Mayer AW (1997). Catechol oxidase from green olives: properties and partial purification. Phytochem 16, 1153-1158.
  • Bradford MA (1976). Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254.
  • Chevalier T, Rigal D, Mbe´guie´-A-Mbe´guie´ D, Gauillard F, Richard-Forget F, Fils-Lycaon B R (1999). Molecular cloning and characterization of apricot fruit poly- phenol oxidase. Plant Phy 119, 1261-1269.
  • Coseteng MY, Lee CY (1978). Changes in apple poly- phenol oxidase and polyphenol concentrations in relation to degree of browning. J Food Sci 52, 985-989.
  • Cuatrecases P (1970). Protein purification by affinity chromotography. J Biochem 245, 3059-3065.
  • Demir H (2004). Purification and characterization of polyphenol oxidase from Erciş apple. Bull Pure Appl Sci C-Chem 23, 15-20.
  • Demir H, Yörük İH, Savran A, Ekici K (2006). Prufication and properties of polyphenol oxidase from Van apple (Golden delicious). Asian J Chem 18, 475-480.
  • Doğan S, Turan Y, Ertürk H, Arslan O (2005). Characterization and purification of polyphenol oxidase from artichoke (Cynara scolymus L.). J Agric Food Chem 53, 776-785.
  • Erat M, Sakiroglu H, Kufrevioglu OK (2006). Purification and characterization of polyphenol oxidase from Ferula sp. Food Chem 95, 503–508.
  • Jharna RD, Santhoor GB, Lalitha RG (1997). Purification and characterization of a polyphenol oxidase from the kew cultivar of Indian pineapple fruit. J Agric Food Chem 45, 2031-2035.
  • Laemmli DK (1970). Cleavage of structural proteins during in asembly of the head of bacteriophage T4. Nature 227, 680-685.
  • Mathew AG, Parpia HAB (1971). Food browning as a polyphenol reaction. Adv Food Res 19, 75-145.
  • Palmer JK (1963). Banana polyphenoxidase prepartion and properties. Phy Plant 38, 508- 513.
  • Schaller K (1972). Zur bestimmung der polyphenol- oksidase aktivat in kartoffelknollen. Z Lebensm Unters Forsch 150, 211-216.
  • Segel IH (1968). Biochemical calculations, second edition, Publised by, John Wiley and Sons Inc. New York, USA, 458 pp., ISBN: 0-471-77421-9.
  • Ünal MÜ (2007). Properties of polyphenol oxidase from Anamur banana (Musa cavendishii). Food Chem 100, 909-913.
  • Wildanger W, Herrmann K (1973). Die phenolicshen inhaltsstofee des obstes. H. die flovonole des obstes. Z Lebensm Unters Forsch 151, 103-108.
  • Valero E, Varon R, Garcia-Carmona F (1988). Characterization of polyphenol oxidase from airen grapes. J Food Sci 53, 1482-1485.
  • Vamos-Vigyazo L (1981). Polyphenol oxidase and peroxidase in fruits and vegetables. Crit Rev Food Sci Nutr 15, 49-127.
  • Yerlitürk FÜ (2003). Peyrus elaegrifolia meyvesinden polifenol oksidaz enziminin afinite kromatogtrafisi ile saflaştırılması incelenemesi. Science Institue, Master Thesis, Balıkesir University, 53 pp. kinetik özelliklerinin
  • Ziyan E, Pekyardımcı S (2004). Purification and characterization of pear (Pyrus communis) polyphenol oxidase. Turk J Chem 28, 547-557.
There are 24 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Fatih Çağlar Çelikezen This is me

Halit Demir This is me

Çilem Çimen This is me

Publication Date June 23, 2012
Submission Date January 16, 2012
Published in Issue Year 2012 Volume: 2 Issue: 1

Cite

IEEE F. Ç. Çelikezen, H. Demir, and Ç. Çimen, “Purification and characterization of polyphenol oxidase enzyme from Iğdır apricot (Prunus armeniaca L.)”, Bitlis Eren University Journal of Science and Technology, vol. 2, no. 1, pp. 22–26, 2012, doi: 10.17678/beuscitech.47148.