Purification and characterization of polyphenol oxidase enzyme from Iğdır apricot (Prunus armeniaca L.)
Year 2012,
Volume: 2 Issue: 1, 22 - 26, 23.06.2012
Fatih Çağlar Çelikezen
Halit Demir
Çilem Çimen
Abstract
In this study, polyphenol oxidase (E.C. 1.14.18.1; PPO) enzyme obtained from Igdir Apricot was purified with method of affinity chromatography. The apricot cultivar “Salak ” was provided from Igdir region. To purify polyphenol oxidase enzyme obtained
References
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- Ziyan E, Pekyardımcı S (2004). Purification and characterization of pear (Pyrus communis) polyphenol oxidase. Turk J Chem 28, 547-557.
Year 2012,
Volume: 2 Issue: 1, 22 - 26, 23.06.2012
Fatih Çağlar Çelikezen
Halit Demir
Çilem Çimen
References
- Anosike EO, Ojimelukwe PC (1982). Partial purification and characterizatin of polyphenol oxidase from cocoyam, Xanthosoma sagitifolium. J Exp Bot 33, 487- 494.
- Aydemir T (2004). Partial purification and characteriza- tion of polyphenol oxidase from artichoke (Cynara scolymus L.). Food Chem 87, 59–67.
- Balasingam K, Ferdinand W (1997). The purification and properties of a ribonucleo enzyme, o-diphenol oxidase, from patotoes. Biochem J 118, 15-23.
- Ben-Shalom N, KahnV, Harl E, Mayer AW (1997). Catechol oxidase from green olives: properties and partial purification. Phytochem 16, 1153-1158.
- Bradford MA (1976). Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254.
- Chevalier T, Rigal D, Mbe´guie´-A-Mbe´guie´ D, Gauillard F, Richard-Forget F, Fils-Lycaon B R (1999). Molecular cloning and characterization of apricot fruit poly- phenol oxidase. Plant Phy 119, 1261-1269.
- Coseteng MY, Lee CY (1978). Changes in apple poly- phenol oxidase and polyphenol concentrations in relation to degree of browning. J Food Sci 52, 985-989.
- Cuatrecases P (1970). Protein purification by affinity chromotography. J Biochem 245, 3059-3065.
- Demir H (2004). Purification and characterization of polyphenol oxidase from Erciş apple. Bull Pure Appl Sci C-Chem 23, 15-20.
- Demir H, Yörük İH, Savran A, Ekici K (2006). Prufication and properties of polyphenol oxidase from Van apple (Golden delicious). Asian J Chem 18, 475-480.
- Doğan S, Turan Y, Ertürk H, Arslan O (2005). Characterization and purification of polyphenol oxidase from artichoke (Cynara scolymus L.). J Agric Food Chem 53, 776-785.
- Erat M, Sakiroglu H, Kufrevioglu OK (2006). Purification and characterization of polyphenol oxidase from Ferula sp. Food Chem 95, 503–508.
- Jharna RD, Santhoor GB, Lalitha RG (1997). Purification and characterization of a polyphenol oxidase from the kew cultivar of Indian pineapple fruit. J Agric Food Chem 45, 2031-2035.
- Laemmli DK (1970). Cleavage of structural proteins during in asembly of the head of bacteriophage T4. Nature 227, 680-685.
- Mathew AG, Parpia HAB (1971). Food browning as a polyphenol reaction. Adv Food Res 19, 75-145.
- Palmer JK (1963). Banana polyphenoxidase prepartion and properties. Phy Plant 38, 508- 513.
- Schaller K (1972). Zur bestimmung der polyphenol- oksidase aktivat in kartoffelknollen. Z Lebensm Unters Forsch 150, 211-216.
- Segel IH (1968). Biochemical calculations, second edition, Publised by, John Wiley and Sons Inc. New York, USA, 458 pp., ISBN: 0-471-77421-9.
- Ünal MÜ (2007). Properties of polyphenol oxidase from Anamur banana (Musa cavendishii). Food Chem 100, 909-913.
- Wildanger W, Herrmann K (1973). Die phenolicshen inhaltsstofee des obstes. H. die flovonole des obstes. Z Lebensm Unters Forsch 151, 103-108.
- Valero E, Varon R, Garcia-Carmona F (1988). Characterization of polyphenol oxidase from airen grapes. J Food Sci 53, 1482-1485.
- Vamos-Vigyazo L (1981). Polyphenol oxidase and peroxidase in fruits and vegetables. Crit Rev Food Sci Nutr 15, 49-127.
- Yerlitürk FÜ (2003). Peyrus elaegrifolia meyvesinden polifenol oksidaz enziminin afinite kromatogtrafisi ile saflaştırılması incelenemesi. Science Institue, Master Thesis, Balıkesir University, 53 pp. kinetik özelliklerinin
- Ziyan E, Pekyardımcı S (2004). Purification and characterization of pear (Pyrus communis) polyphenol oxidase. Turk J Chem 28, 547-557.