Effective Contamination Agent For Tissue Culture Applications of Bacopa monnieri

Abstract

Biocides and plant protection products have been used in plant tissue culture sterilization procedures since they have broad spectrum, are inexpensive and resistant to autoclave process. This study was conducted to determine the effects of contamination agents on tissue culture applications. MS medium was supplied with 0.5mg/l 6-benzylaminopurine and 0.1 mg/L indole-3-butyric acid. PPM and Contaminacide and their three dosages (0, 3 and 6 ml/L) were used for maintaining tissue culture aseptic conditions. Contamination rate and plant growth parameters were subjected. after lids remained open for three days in Bacopa monnieri micropropagation conditions. Although preservation-free and 3mlL-1 PPM-added media were contaminated, contamination did not occur in all dosages of Contaminacide and 6 ml/L PPM. In conclusion 3ml/L Contaminacide added media were superior in terms of contamination rate and plant height, fresh and dry weight (LSD;p<0.05).

Keywords

• Sterilization
• micropropagation
• fungi
• bacteria
• PPM
• Contaminacide

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