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Ratlarda Cerrahi Uygulamalarda Adezyon Oluşumu ve Bazı Hematolojik Değerler Üzerine Heparin ve Pentoxifylline’nin Etkisi

Year 2023, , 205 - 212, 31.12.2023

Halil Şimşek , Ali Said Durmuş , Hamit Yıldız

https://doi.org/10.58605/bingolsaglik.1390878

Abstract

Bu çalışmada ratlarda yapışıklığın önlenmesinde Heparin ve Pentoksifilin’in hematolojik değerlerde etkisinin belirlenmesi amaçlandı.
Çalışma gruplarından; Sham (Sh) grubuna laparatomi yapıldı ve periton içi %0,9 2 mL NaCl solüsyonu verildi. Kontrol (K), heparin (H) ve pentoksifilin (PTX) grubundaki ratlara uygulama sonrası; K grubuna %0,9 2 mL NaCl solüsyonu, H grubuna 500 IU heparin, PTX grubuna ise Pentoksifilin damlatıldı ve alan kapatıldı. Uygulama sonrası hayvanlara laparotomi yapıldı ve adezyon oluşumu derecelendirildi. Alınan kan örneklerinde hematolojik değerler incelendi. Çalışmada gruplar arası ortalama eritrosit düzeyinde farkın olduğu bu farkın Sh grubu ile K, H ve PTX grupları arasında istatistiksel açıdan önemli olduğu saptandı. Lökosit düzeyinin Sh grubunda diğer gruplardan istatistiksel yönden önemli olduğu saptandı. Hematokrit düzeyinin, K grubunda diğer gruplardan düşük oranda olduğu ve değerin istatistiksel olarak anlamlı olduğu tespit edildi. Ortalama alyuvar hacminin Sh ile H grubu arasındaki farkın istatistiksel yönden önemli olduğu saptandı. Nötrofil sayısında gruplar arasındaki farkın istatistiksel açıdan anlamlı olduğu gözlendi. Diğer kan parametrelerinde ise gruplar arasında farkın bulunduğu ancak farkın istatistiksel açıdan önemli olmadığı tespit edildi.

Keywords

Rat Heparin Adezyon

References

  • Arung W, Meurisse M, Detry O. Pathophysiology and prevention of postoperative peritoneal adhesions. World J Gastroenterol. 2011; 17: 4545-4553.
  • Houston DE, Noller K, Melton LJ, Selwyn BJ. The epidemiology of pelvic endometriosis. Clin Obstet Gynecol. 1988; 31(4): 787- 800.
  • Missmer SA, Cramer DW. The epidemiology of endometrioisis. Obstet Gynecol Clin North Am. 2003; 30(1): 1-19.
  • Coleman MG, McLain AD, Moran BJ. Impact of previous surgery on time taken for incision and division of adhesions during laparotomy. Dis Colon Rectum. 2000; 43: 1297-1299.
  • Remah MK. Prevention of postoperative peritoneal adhesions. Eur J Obstet Gynecol Reprod Biol. 2010; 150: 111-118.
  • Nehéz L, Vödrös D, Axelsson J, Tingstedt B, Lindman B, Andersson R. Prevention of postoperative peritoneal adhesions: Effects of lysozyme, polylysine and polyglutamateversus hyaluronicacid. Scand J Gastroentero. 2005; 40: 1118-1123.
  • Metwally M, Watson A, Lilford R, Vandekerckhove P. Fluid and pharmacological agents for adhesion prevention after gynaecological surgery. Cochrane Database Syst Rev. 2006; 19(2): CD001298.
  • Galili Y, Ben Abraham R, Rabau M, Klausner J, Kluger Y. Reduction of surgery induced peritoneal adhesions by methylene blue. Am J Surg. 1998; 175: 30-32.
  • Bahadir L, Oncel M, Kement M, Sahip Y. Intra-abdominal use of taurolidine or heparin as alternative products to an antiadhesive barrier (Seprafilm®) in adhesion prevention: An experimental study on mice. Dis Colon Rectum. 2007; 50: 2209-2214.
  • Arıkan S, Adas G, Barut G, Toklu AS, Kocakuşa, A, Uzun H, Kemik Ö, Daduk Y, Aydın S, Purisa S. An evaluation of low molecular weight heparin anf hyperbaric oxygen treatment in the prevention of intra-abdominal adhesions and wound healing. Am J Surg. 2005; 189: 155-160.
  • Gandhi NS, Mancera RL. The structure of glycosaminoglycans and their interactions with proteins. Chem Biol Drug Des. 2008; 72: 455-482.
  • Kutlay J, Ozer Y, Isik B, Kargici H. Comparative effectiveness of several agents for preventing postoperative adhesions. World J Pediatr Surg. 2004; 28: 662-665.
  • Balasch J, Creus M, Fabregues F, Carmona F, Martinez-Roman S, Manau D, Vanrell JA. Pentoxifylline versus plecebo in the treatment of infertility associated with minimal or mild endometriosis: A pilot randomized clinical trial. Humn Reprod. 1997; 12(9): 2046-2050.
  • Aytan H, Caglar P, Uygur D, Zeteroglu S, Batioglu S. Effect of immunmodulator leflunamide on the induction endometriosis in experimental rat model. Fertil Steril. 2007; 87(3): 699-702.
  • Sulliva GW, Carper HT, Novick WJ, Mandel GL. Inhibition of The Inflamatory Action of IL- 1 and TNF on Neutrophil By Pentoxifylline. Infect Immun. 1988; 56(7): 1722.
  • Okumura AS, Rodrigues LE, Martinelli R. Pentoxifylline in ischemia-induced acute kidney injury in rats. Renal Failure. 2009; 31: 829-832.
  • Nair SK, Bhat IK, Aurora AR. Role of proteolytic enzymes in the prevention of postoperative intraabdominal adhesions. Arch Surg. 1974; 108: 849-853.
  • Konuk T. Pratik fizyoloji I. Ankara: Ankara Üniversitesi, Veteteriner Fakültesi Yayınları. 1975.
  • Schalm OW. Veterinary hematology. Philadelphia, Leaand Febiger. 1971.
  • 20. Cannan RK. Hemoglobin (As Cyanmethemoglobin) in blood. Clinical Chemistry. 1958; 4: 246-251.
  • Yıldız H, Şimşek H. İneklerde östrüs siklusunun farklı dönemleri boyunca bazı hematolojik değerler. Turk J Vet Anim Sci. 2003; 27: 1305-1309.
  • Sümbüloğlu K, & Sümbüloğlu V. Biyoistatistik. Özdemir Basım Yayım ve Dağıtım LTD. Şti. 1995.
  • Şimşek H, Durmuş AS, Yıldız H, Özçelik M. Surgery-induced changes in erythrocyte and plasma lipid peroxidation, enzymatic and non-enzymatic antioxidants of female rats: Protective role of heparin and pentoxifylline. Acta Scientiae Veterinariae. 2018; 46: 1619.
  • Perry JF, Smith AG, Yonethiro EG. Intestinal obstruction caused by adhesion. Annals of Surgery. 1995; 142: 810-816.
  • Drollette CM, Badawy SZ. Pathophysiology of pelvic adhesions. Modern trends in preventing infertility. J Reprod Med. 1992; 37(2): 107-121.
  • Fukasawa M, Girgis W, diZerega GS. Inhibition of post surgical adhesions in a standardized rabbit model. II. Intraperitoneal treatment with heparin. Int J Fertil. 1991; 36(5): 296-301.
  • Yovich JM, Ediringhe WR, Cummins JM, Yovich JL. Influence of pentoxifylline in pelvic peritoneal macrophages. Fertility and Sterility. 1990; 46: 715-722.
  • Lai HS, Chu SY, Chen Y, Wu CH, Lin LT. Effect of pentoxifylline on intraperitoneal adhesion safter intestinal resection in rats. J Formos Med Assoc. 1994; 93: 911-915.
  • Basbug M, Aygen E, Tayyar M, Kaya E, Narin F, Oktem O. Hyaluronic acid plus heparin for improved efficacy in prevention of adhesion formation in rat uterine horn model. Eur J Obstet Gynecol Reprod Biol. 1998; 78: 109-112.
  • Steinleitner A, Lambert H, Kazensky C, Danks P, Roy S. Pentoxifylline, a methylxanthine derivative, prevents postsurgical adhesion reformation in rabbits. Obstetrics & Gynecology. 1990; 75: 926-928.
  • Tarhan O, Barut I, Sütcü R, Akdeniz Y, Aktürk O. Pentoxifylline, a methly xanthine derivative, reduces peritoneal adhesions and increases peritoneal fibrinolysis in rats. Tohoku J Exp Med. 2006; 209: 249-255.
  • Hernandez Villaroel LA, Cesin L, Fernandez H. Pentoxifylline effects on the formation of postoperative peritoneal adhesions in rats. Rev Cient Cienc Méd. 2016; 19(1): 5-11.
  • Kement M, Censur Z, Oncel M, Büyükokuroğlu ME, Gezen FC. Heparin for adhesion prevention: Comparison of three different dosages with seprafilm in a murine model. Int J Surg. 2011; 9: 225-228.
  • Liakakos T, Thomakos N, Fine PM, Dervenis C, Young RL. Peritoneal adhesions: Etiology, pathophysiology and clinical significance. Recent advances in prevention and management. Dig Surg. 2001; 18: 260-273.
  • Yang KD, Hill RH. Neutrophill function disordes: Pathophysiology, prevention and therapy. J Pediadr. 1991; 119(3): 343-354.
  • Rahman J, Meilsp J. Dynamic exercise leads an to increase in circulating ICAM–1 further evidence per adrenergic modullation of cell adhesion. Brain Behav Immun. 1997; 11: 343-351.
  • Saygılı Ö, Gültekin F. Hücre adezyon molekülleri. Turkiye Klinikleri J Med Sci. 1999; 19: 362-365.
  • Peach RJ, Hollenbaugh D, Stamenkovic I, Aruffo A. Identification of hyaluronic acid binding sites in the extracellular domain of CD44. J Cell Biol. 1993; 122: 257-264.
  • Mackay CR, Imhof BA. Cell adhesion in the immune system. Immunology Today. 1993; 14: 99-102.
  • Ergüler G, Demir N, Demir R. Adezyon moleküllerinin yapısal özellikleri ve fonksiyonları. Turkiye Klinikleri J Med Sci. 2002; 22: 313–327.

Year 2023, , 205 - 212, 31.12.2023

Halil Şimşek , Ali Said Durmuş , Hamit Yıldız

https://doi.org/10.58605/bingolsaglik.1390878

Abstract

References

  • Arung W, Meurisse M, Detry O. Pathophysiology and prevention of postoperative peritoneal adhesions. World J Gastroenterol. 2011; 17: 4545-4553.
  • Houston DE, Noller K, Melton LJ, Selwyn BJ. The epidemiology of pelvic endometriosis. Clin Obstet Gynecol. 1988; 31(4): 787- 800.
  • Missmer SA, Cramer DW. The epidemiology of endometrioisis. Obstet Gynecol Clin North Am. 2003; 30(1): 1-19.
  • Coleman MG, McLain AD, Moran BJ. Impact of previous surgery on time taken for incision and division of adhesions during laparotomy. Dis Colon Rectum. 2000; 43: 1297-1299.
  • Remah MK. Prevention of postoperative peritoneal adhesions. Eur J Obstet Gynecol Reprod Biol. 2010; 150: 111-118.
  • Nehéz L, Vödrös D, Axelsson J, Tingstedt B, Lindman B, Andersson R. Prevention of postoperative peritoneal adhesions: Effects of lysozyme, polylysine and polyglutamateversus hyaluronicacid. Scand J Gastroentero. 2005; 40: 1118-1123.
  • Metwally M, Watson A, Lilford R, Vandekerckhove P. Fluid and pharmacological agents for adhesion prevention after gynaecological surgery. Cochrane Database Syst Rev. 2006; 19(2): CD001298.
  • Galili Y, Ben Abraham R, Rabau M, Klausner J, Kluger Y. Reduction of surgery induced peritoneal adhesions by methylene blue. Am J Surg. 1998; 175: 30-32.
  • Bahadir L, Oncel M, Kement M, Sahip Y. Intra-abdominal use of taurolidine or heparin as alternative products to an antiadhesive barrier (Seprafilm®) in adhesion prevention: An experimental study on mice. Dis Colon Rectum. 2007; 50: 2209-2214.
  • Arıkan S, Adas G, Barut G, Toklu AS, Kocakuşa, A, Uzun H, Kemik Ö, Daduk Y, Aydın S, Purisa S. An evaluation of low molecular weight heparin anf hyperbaric oxygen treatment in the prevention of intra-abdominal adhesions and wound healing. Am J Surg. 2005; 189: 155-160.
  • Gandhi NS, Mancera RL. The structure of glycosaminoglycans and their interactions with proteins. Chem Biol Drug Des. 2008; 72: 455-482.
  • Kutlay J, Ozer Y, Isik B, Kargici H. Comparative effectiveness of several agents for preventing postoperative adhesions. World J Pediatr Surg. 2004; 28: 662-665.
  • Balasch J, Creus M, Fabregues F, Carmona F, Martinez-Roman S, Manau D, Vanrell JA. Pentoxifylline versus plecebo in the treatment of infertility associated with minimal or mild endometriosis: A pilot randomized clinical trial. Humn Reprod. 1997; 12(9): 2046-2050.
  • Aytan H, Caglar P, Uygur D, Zeteroglu S, Batioglu S. Effect of immunmodulator leflunamide on the induction endometriosis in experimental rat model. Fertil Steril. 2007; 87(3): 699-702.
  • Sulliva GW, Carper HT, Novick WJ, Mandel GL. Inhibition of The Inflamatory Action of IL- 1 and TNF on Neutrophil By Pentoxifylline. Infect Immun. 1988; 56(7): 1722.
  • Okumura AS, Rodrigues LE, Martinelli R. Pentoxifylline in ischemia-induced acute kidney injury in rats. Renal Failure. 2009; 31: 829-832.
  • Nair SK, Bhat IK, Aurora AR. Role of proteolytic enzymes in the prevention of postoperative intraabdominal adhesions. Arch Surg. 1974; 108: 849-853.
  • Konuk T. Pratik fizyoloji I. Ankara: Ankara Üniversitesi, Veteteriner Fakültesi Yayınları. 1975.
  • Schalm OW. Veterinary hematology. Philadelphia, Leaand Febiger. 1971.
  • 20. Cannan RK. Hemoglobin (As Cyanmethemoglobin) in blood. Clinical Chemistry. 1958; 4: 246-251.
  • Yıldız H, Şimşek H. İneklerde östrüs siklusunun farklı dönemleri boyunca bazı hematolojik değerler. Turk J Vet Anim Sci. 2003; 27: 1305-1309.
  • Sümbüloğlu K, & Sümbüloğlu V. Biyoistatistik. Özdemir Basım Yayım ve Dağıtım LTD. Şti. 1995.
  • Şimşek H, Durmuş AS, Yıldız H, Özçelik M. Surgery-induced changes in erythrocyte and plasma lipid peroxidation, enzymatic and non-enzymatic antioxidants of female rats: Protective role of heparin and pentoxifylline. Acta Scientiae Veterinariae. 2018; 46: 1619.
  • Perry JF, Smith AG, Yonethiro EG. Intestinal obstruction caused by adhesion. Annals of Surgery. 1995; 142: 810-816.
  • Drollette CM, Badawy SZ. Pathophysiology of pelvic adhesions. Modern trends in preventing infertility. J Reprod Med. 1992; 37(2): 107-121.
  • Fukasawa M, Girgis W, diZerega GS. Inhibition of post surgical adhesions in a standardized rabbit model. II. Intraperitoneal treatment with heparin. Int J Fertil. 1991; 36(5): 296-301.
  • Yovich JM, Ediringhe WR, Cummins JM, Yovich JL. Influence of pentoxifylline in pelvic peritoneal macrophages. Fertility and Sterility. 1990; 46: 715-722.
  • Lai HS, Chu SY, Chen Y, Wu CH, Lin LT. Effect of pentoxifylline on intraperitoneal adhesion safter intestinal resection in rats. J Formos Med Assoc. 1994; 93: 911-915.
  • Basbug M, Aygen E, Tayyar M, Kaya E, Narin F, Oktem O. Hyaluronic acid plus heparin for improved efficacy in prevention of adhesion formation in rat uterine horn model. Eur J Obstet Gynecol Reprod Biol. 1998; 78: 109-112.
  • Steinleitner A, Lambert H, Kazensky C, Danks P, Roy S. Pentoxifylline, a methylxanthine derivative, prevents postsurgical adhesion reformation in rabbits. Obstetrics & Gynecology. 1990; 75: 926-928.
  • Tarhan O, Barut I, Sütcü R, Akdeniz Y, Aktürk O. Pentoxifylline, a methly xanthine derivative, reduces peritoneal adhesions and increases peritoneal fibrinolysis in rats. Tohoku J Exp Med. 2006; 209: 249-255.
  • Hernandez Villaroel LA, Cesin L, Fernandez H. Pentoxifylline effects on the formation of postoperative peritoneal adhesions in rats. Rev Cient Cienc Méd. 2016; 19(1): 5-11.
  • Kement M, Censur Z, Oncel M, Büyükokuroğlu ME, Gezen FC. Heparin for adhesion prevention: Comparison of three different dosages with seprafilm in a murine model. Int J Surg. 2011; 9: 225-228.
  • Liakakos T, Thomakos N, Fine PM, Dervenis C, Young RL. Peritoneal adhesions: Etiology, pathophysiology and clinical significance. Recent advances in prevention and management. Dig Surg. 2001; 18: 260-273.
  • Yang KD, Hill RH. Neutrophill function disordes: Pathophysiology, prevention and therapy. J Pediadr. 1991; 119(3): 343-354.
  • Rahman J, Meilsp J. Dynamic exercise leads an to increase in circulating ICAM–1 further evidence per adrenergic modullation of cell adhesion. Brain Behav Immun. 1997; 11: 343-351.
  • Saygılı Ö, Gültekin F. Hücre adezyon molekülleri. Turkiye Klinikleri J Med Sci. 1999; 19: 362-365.
  • Peach RJ, Hollenbaugh D, Stamenkovic I, Aruffo A. Identification of hyaluronic acid binding sites in the extracellular domain of CD44. J Cell Biol. 1993; 122: 257-264.
  • Mackay CR, Imhof BA. Cell adhesion in the immune system. Immunology Today. 1993; 14: 99-102.
  • Ergüler G, Demir N, Demir R. Adezyon moleküllerinin yapısal özellikleri ve fonksiyonları. Turkiye Klinikleri J Med Sci. 2002; 22: 313–327.
There are 40 citations in total.

Details

Primary Language Turkish
Subjects Surgical Diseases Nursing​​
Journal Section Araştırma Makaleleri
Authors

Halil Şimşek 0000-0002-9637-1265

Ali Said Durmuş 0000-0003-4873-5690

Hamit Yıldız 0000-0001-7858-5123

Early Pub Date December 30, 2023
Publication Date December 31, 2023
Submission Date November 15, 2023
Acceptance Date December 15, 2023
Published in Issue Year 2023

Cite

Vancouver Şimşek H, Durmuş AS, Yıldız H. Ratlarda Cerrahi Uygulamalarda Adezyon Oluşumu ve Bazı Hematolojik Değerler Üzerine Heparin ve Pentoxifylline’nin Etkisi. BÜSAD. 2023;4(2):205-12.