Matricaria recutita L. capitulum eksplantının in vitro rejenerasyon potansiyeli

Abstract

Özet Amaç: Bu çalışmada, tıbbi ve aromatik bir bitki olan Matricaria recutita L.' nin in vitro rejenerasyon potansiyeli araştırılmıştır. Metod: Tarla koşullarında yetiştirilen bitkilerden izole edilen kapitulum eksplantlarının sterilizasyon protokolünü belirlemek amacıyla altı farklı sterilizasyon işlemi test edildi. Rejenerasyon çalışmaları için kapitulum eksplantlarına, 24 farklı konsantrasyon ve kombinasyonda BAP (0,5 -1-2 mg/L) ve NAA (0-0,5-1-2 mg/L); KIN (0,5-1-2 mg/L) ve 2,4- D (0-0,5 mg/L) bitki büyüme düzenleyicileri uygulanmıştır. Bulgular: Sterilizasyon amaçlı yapılan deneylerde en iyi sonuçlar, %70’lik etanolde 3 dakika, %30’luk NaOCl’de 10 dakika ve %1’lik AgNO3’te 20 ile elde edilmiştir. Rejenerasyon çalışmaları sonucunda; en iyi sürgün uzunlukları sırasıyla 0,5 mg/L BAP+0,5 mg/L NAA ve 0,5 mg/L BAP+2 mg/L NAA içeren MS besin ortamlarında 3,02 ve 3,00 cm olarak belirlenirken; en iyi eksplant başına sürgün sürgün sayısı sırasıyla 0,5 mg/L BAP+0,5 mg/L NAA ve 0,5 mg/L BAP+2 mg/L NAA bitki büyüme düzenleyicileri içeren MS besin ortamlarında 23,91 ve 18,29 olarak belirlenmiştir. Sonuç: In vitro rejenerasyon çalışmalarından elde edilen sonuçlara göre uygulanan bitki büyüme düzenleyici kombinasyonları açısından, BAP ve NAA; KIN ve 2,4-D uygulamalarına göre daha başarılı sonuçlar vermiştir.

Keywords

• bitki büyüme düzenleyiciler
• Bitki doku kültürü
• oksin
• sitokinin
• Matricaria recutita L.

In vitro rejeneration potential of Matricaria recutita L. capitulum explant

Abstract

Purpose: In this study, the in vitro regeneration potential of Matricaria recutita L., a medicinal and aromatic plant, was investigated Method: Six different sterilization processes were tested to determine the sterilization protocol of capitulum explants isolated from plants grown under field conditions. For regeneration studies, plant growth regulators BAP (0.5-1-2 mg/L) and NAA (0-0.5-1-2 mg/L); KIN (0.5-1-2 mg/L) and 2.4-D (0-0.5 mg/L) were applied to capitulum explants at 24 different concentrations and combinations. Findings: In the experiments carried out for sterilization purposes, the best results were obtained in 70% ethanol for 3 minutes, in 30% NaOCl for 10 minutes and in 1% AgNO3 for 20 minutes. The best shoot lengths were determined as 3.02 and 3.00 cm in MS nutrient medium containing 0.5 mg/L BAP+0.5 mg/L NAA and 0.5 mg/L BAP+2 mg/L NAA, respectively; the best shoot number per explant was determined as 23.91 and 18.29 in MS nutrient medium containing 0.5 mg/L BAP+0.5 mg/L NAA and 0.5 mg/L BAP+2 mg/L NAA plant growth regulators, respectively. Conclusion: According to the results obtained from in vitro regeneration studies, in terms of the applied plant growth regulator combinations, BAP and NAA gave more successful results than KIN and 2.4-D applications

Keywords

• auxin
• cytokinin
• Matricaria recutita L.
• plant growth regulator
• Plant tissue culture

References

1. [1] Salamon, I., Ibraliu, A., & Kryvtsova, M. (2023). Essential oil content and composition of the Chamomile inflorescences (Matricaria recutita L.) Belonging to Central Albania. Horticulturae, 9(1), 1-13. https://doi.org/10.3390/horticulturae9010047
2. [2] Khazaneha, M., Zandrahimi, F., Sadatmoosavi, A., Salarpour, S., Karegar-Borzi, H., Tajedini, O., ... & Raisszadeh, A. (2024). An overview of scientific publication of the Chamomile (Matricaria chamomilla) research: A bibliometric analysis. Journal of Scientometric Research, 13(2), 604-614. https://doi.org/10.5530/jscires.13.2.47
3. [3] Srivastava, J. K., Shankar, E., & Gupta, S. (2010). Chamomile: a herbal medicine of the past with a bright future. Molecular Medicine Reports, 3(6), 895-901. https://doi.org/10.3892/mmr.2010.377
4. [4] Salehi, A., Gholamhoseini, M., Ataei, R., Sefikon, F., & Ghalavand, A. (2018). Effects of zeolite, bio-and organic fertilizers application on german chamomile yield and essential oil composition. Journal of Essential Oil Bearing Plants, 21(1), 116-130. https://doi.org/10.1080/0972060X.2018.1436985
5. [5] Tanrıkulu N. (2024). Comparative research of findings of some medicinal plants in tradiotional medicine, folk medicine, and scientific. Biological Diversity and Conservation, 17(3), 267-280. https://doi.org/10.46309/biodicon.2024.1232520
6. [6] Miraj, S., & Alesaeidi, S. (2016). A systematic review study of therapeutic effects of Matricaria recuitta chamomile (chamomile). Electronic physician, 8(9), 3024. 10.19082/3024
7. [7] Batovska, D., Panova, N., Gerasimova, A., Tumbarski, Y., Ivanov, I., Dincheva, I., ... & Nikolova, K. (2025). Chamomile matters: species-and producer-dependent variation in Bulgarian Matricaria recutita L. and Chamaemelum nobile L. essential oils and their cosmetic potential. Cosmetics, 12(3), 123. https://doi.org/10.3390/cosmetics12030123
8. [8] Mailänder, L. K., Lorenz, P., Bitterling, H., Stintzing, F. C., Daniels, R., & Kammerer, D. R. (2022). Phytochemical characterization of Chamomile (Matricaria recutita L.) roots and evaluation of their antioxidant and antibacterial potential. Molecules, 27(23), 8508. https://doi.org/10.3390/molecules27238508

9. [9] Singh, O., Khanam, Z., Misra, N., & Srivastava, M. K. (2011). Chamomile (Matricaria chamomilla L.): an overview. Pharmacognosy reviews, 5(9), 82.
10. [10] El-Sherif, N. A. (2018). Impact of plant tissue culture on agricultural sustainability. In Sustainability of Agricultural Environment in Egypt: Part II: Soil-Water-Plant Nexus (pp. 93-107). Cham: Springer International Publishing.
11. [11] Kocaçalışkan, İ. (2017). Doku ve Hücre Kültürü Teknikleri [Tissue and Cell Culture Techniques], İstanbul: Nobel Yayın Dağıtım.
12. [12] Gürel, A., Hayta, Ş., Nartop, P., Bayraktar, M. ve Orhan-Fedakar, S., (2013). Bitki hücre, doku ve organ kültürü uygulamaları [Plant cell, tissue and organ culture applications], İzmir: Ege Üniversitesi Yayınları.
13. [13] Bidabadi, S. S., & Jain, S. M. (2020). Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration. Plants, 9(6), 702, 1-20. https://doi.org/10.3390/plants9060702
14. [14] Murashige T., Skoog F., (1962), A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol Plant, 15, 473-497.
15. [15] SPPS; IBM SPSS Statistics for Windows, version 24 (IBM Corp., Armonk, NY, ABD).
16. [16] Mihaljević, I., Dugalić, K., Tomaš, V., Viljevac, M., Pranjić, A., Čmelik, Z., & Jurković, Z. (2013). In vitro sterilization procedures for micropropagation of ‘Oblačinska’sour cherry. Journal of Agricultural Sciences, Belgrade, 58(2), 117-126. https://doi.org/10.2298/JAS1302117M
17. [17] Gerszberg, A., & Grzegorczyk-Karolak, I. (2019). Influence of selected antibiotics on the tomato regeneration in in vitro cultures. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3), 558-564. https://doi.org/10.15835/nbha47311401
18. [18] Rahman, N. N. A., Rosli, R., Kadzimin, S., & Hakiman, M. (2019). Effects of auxin and cytokinin on callus induction in Catharanthus roseus (L.) G. Don. Fundamental and Applied Agriculture, 4(3), 928-932. https://doi.org/10.5455/faa.54779 [19] Taniguchi, K., & Takano, H. (1997). Micropropagation of Matricaria chamomilla L.(Camomile). In High-Tech and Micropropagation VI (pp. 332-342). Berlin, Heidelberg: Springer Berlin Heidelberg.
19. [20] Echeverrigaray, S., Fracaro, F., Andrade, L. B., Biasio, S., & Atti-Serafini, L. (2000). In vitro shoot regeneration from leaf explants of Roman Chamomile. Plant cell, tissue and organ culture, 60(1), 1-4. https://doi.org/10.1023/A:1006368210670
20. [21] Tiwari, V., Tiwari, K. N., & Singh, B. D. (2001). Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. Plant cell, tissue and organ culture, 66(1), 9-16. https://doi.org/10.1023/A:1010652006417
21. [22] Bhalla, S., Abdullah, J. O., Sreeramanan, S., & Karuthan, C. (2009). Shoots induction from Hibiscus rosa-sinensis nodal explant using N6-benzylaminopurine (BAP). Research Journal of Agriculture and Biological Sciences, 5(4), 403-410.
22. [23] Ahmad, A., ul Qamar, M. T., Shoukat, A., Aslam, M. M., Tariq, M., Hakiman, M., & Joyia, F. A. (2021). The effects of genotypes and media composition on callogenesis, regeneration and cell suspension culture of chamomile (Matricaria chamomilla L.). PeerJ, 9, e11464.