Karides atıklarından biyolojik kitosan üretimi ve üretilen kitosanın anti-mikrobiyal aktivitesinin değerlendirilmesi

Abstract

Amaç: Biyolojik bir polimer olan kitosan tıbbi, tarımsal ve endüstriyel uygulamalar gibi pek çok alanda kullanılabilen önemli bir polisakkarittir. Sanayide mevcut üretim yöntemleri yoğun kimyasal madde içermektedir, çevreye zarar vermektedir ve yüksek maliyetlidir. Bu durum sürdürülebilir üretim tekniklerinin geliştirilmesinin önemine dikkat çeker. Kitosan, kitinin deasetilasyonundan elde edilir. Deniz bakterileri, kitin bakımından zengin ortamlarda yaşarlar. Dolayısıyla kitine doğal afiniteleri ve yüksek enzim aktiviteleri nedeniyle kitosanın sürdürülebilir üretiminde kıymetli enzimatik üreticilerdir. Metod: Bu çalışmada, kitin bakımından zengin atık karides kabuklarından enzimatik yöntemle kitosan sentezlenmiştir. Marmara Denizi'nden izole edilen 10 bakteri arasından en yüksek üretim verimliliğini gösteren yedi aday seçilmiştir. Kitosan doğrulaması P-nitroasetanilid, iyodür indükleyicileri ve FT-IR analizi ile elde edildi. Bulgular: Araştırma, Marmara Denizi deniz bakterilerinin kimyasal sentezden üç ila dört kat daha yüksek seviyelerde kitosan ürettiğini ve sürdürülebilir ve uygun maliyetli bir endüstriyel çözüm sunduğunu başarıyla göstermektedir. Sonuç: Biyolojik olarak üretilen kitosanın üstün antimikrobiyal özellikleri, biyomedikal ve çevresel uygulamalar için önemli potansiyelinin altını çizmektedir.

Keywords

• kitin
• kitosan
• deniz bakterisi
• antimikrobial aktivite

Biological chitosan production from shrimp waste and evaluation of anti-microbial activity of produced chitosan

Abstract

Purpose: Chitosan, a biological polymer, is an important polysaccharide that can be used in many fields such as medical and industrial applications. Current production methods in the industry are chemical intensive, environmentally damaging and costly. This situation emphasizes the importance of developing sustainable production techniques. Chitosan is obtained from the deacetylation of chitin. Marine bacteria live in chitin-rich environments. Therefore, they are valuable enzymatic producers for sustainable chitosan production due to their natural affinity for chitin and high enzyme activity. Method: In this study, chitosan was synthesized from chitin-rich waste shrimp shells by enzymatic method. Seven candidates showing the highest production efficiency were selected from 10 bacteria isolated from the Marmara Sea. Chitosan validation was obtained by P-nitroacetanilide, iodide inducers and FT-IR analysis. Findings: The research successfully demonstrates that Marmara Sea marine bacteria produce chitosan at higher levels than chemical synthesis, offering a sustainable and cost-effective industrial solution. Conclusion: The superior antimicrobial properties of biologically produced chitosan underline its significant potential for biomedical and environmental applications.

Keywords

• chitin
• chitosan
• marine bacteria
• antimicrobial activity

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