Soğuksu Ulusal Park’ta yayılış gösteren Odiellus lendlii (Soerensen, 1894)’nin mitokondriyal sitokrom c oksidaz altünite I, 16S rRNA, 18S rRNA, ITS1/ ITS2 gen markırları temelli moleküler filogenetik analizi

Abstract

Amaç: Ankara Soğuksu Milli Parkı’nda yayılış gösteren Phalangiidae familyasına ait Odiellus lendlii türünün moleküler karakterizasyonunu DNA barkodlama temelli yöntemlerle araştırmak. Metod: Haziran 2022 ile Eylül 2023 tarihleri arasında toplanan Odiellus lendlii örnekleriyle DNA izolasyonu gerçekleştirilmiştir. Ardından, mitokondriyal COI, 18S rRNA, 16S rRNA, ITS1 ve ITS2 genlerinin primerleri kullanılarak PCR ve DNA dizi analizi yapılmıştır. Chromas ile elde edilen FASTA dizileri, NCBI BLAST veri tabanındaki diğer Phalangiidae veya Odiellus türlerinin dizileriyle karşılaştırılmıştır. Filogenetik analiz için diziler MEGA (v.7.0) programına yüklenmiş ve Maksimum Likelihood yöntemi, Tamura-Nei/Jukes Cantor Modeli, Bootstrap 1000 ile filogenetik ağaçlar oluşturulmuştur. Bulgular: Çalışma, Ankara Soğuksu Milli Parkı’nda yayılış gösteren Odiellus lendlii üzerine yapılan ilk moleküler çalışmayı oluşturmaktadır ve GenBank’a bir kayıt sağlamaktadır. Sonuç: Elde edilen moleküler veriler, bu tür için bu bölgede genetik karakterizasyon ve barkod tabanlı referans oluşturulmasına katkıda bulunmakta olup gelecekteki taksonomi ve ekoloji çalışmalarını desteklemektedir.

Keywords

• Odiellus lendlii
• COI
• 18S rRNA
• 16S rRNA
• ITS1
• ITS2

Molecular phylogenetic analysis of Odiellus lendlii (Soerensen, 1894) from Soğuksu National Park, based on Mitochondrial Cytochrome c oxidase subunit I, 16S rRNA, 18S rRNA, and ITS1/ ITS2 gene markers

Abstract

Purpose: The study aims to characterize molecularly the species Odiellus lendlii of the family Phalangiidae, which occurs in Ankara Soguksu National Park, using DNA barcoding-based methods. Method: Samples of Odiellus lendlii collected between June 2022 and September 2023 were used for DNA isolation. PCR and DNA sequencing were performed targeting mitochondrial COI, 18S rRNA, 16S rRNA, ITS1, and ITS2 gene regions using specific primers. FASTA sequences obtained with Chromas were compared to sequences of other Phalangiidae or Odiellus species in the NCBI BLAST database. For phylogenetic analysis, sequences were uploaded to MEGA (v.7.0); the Maximum Likelihood method with Tamura-Nei/Jukes-Cantor model and bootstrap analysis (1000 replicates) was used to construct phylogenetic trees. Findings: This study constitutes the first molecular investigation on Odiellus lendlii distributed in Ankara Soguksu National Park and provides a GenBank submission. Conclusion: The molecular data obtained contribute to the genetic characterization and barcoding reference for Odiellus lendlii in this region, supporting future taxonomic and ecological studies.

Keywords

• Odiellus lendlii
• COI
• 18S rRNA
• 16S rRNA
• ITS1
• ITS2

References

1. [1] Museu Nacional/UFRJ (2022). Checklist of valid genera of Opiliones of the World 2002. http://www.museunacional. ufrj.br/mndi/Aracnologia/ checklaniator.htm Date Accession: 04.11.2025
2. [2] Cokendolpher, J.C. & Holmberg RG. 2018. Harvestmen of the family Phalangiidae (Arachnida, Opiliones) in the Americas. Special Publications, Museum of Texas Tech University. No. 67:1–44. https://doi:10.1636/JoA-S-22-007
3. [3] Cokendolpher, J.C. (1993). Pathogens and parasites of opiliones (Arthropoda: Arachnida). The Journal of Arachnology, 21: 120-146. https://doi:10.5281/zenodo.16183208
4. [4] Hicks, B.J., McKenzie, F., Cosens, D., & Watt A.D. (2003). Harvestmen abundance and diversity within lodgepole and Scots pine plantations of Scotland and their impact on pine beauty moth populations. Forest Ecology and Management, 182 (1-3), 355-361. https://doi.org/10.1016/S0378-1127(03)00093-8.
5. [5] Lindtner P., Gajdoš P., Stašiov, S., Čiliak, M., Pech, P. & Kubovčík V. (2020) Spider (Araneae) and harvestman (Opiliones) communities arestructured by the ecosystem engineering of burrowing mammals. Insect Conserv Divers 13:262–270. https://doi.org/10.1111/icad.12382
6. [6] Hillyard, P.D. & Sankey, J.H.P. (1989). Synopses of the British Fauna (New Series).
7. [7] Folmer, O., Black, M., Hoeh, W., Lutz, R. &Vrijenhoef, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294-299. https://doi.org/10.1371/journal.pone.0013102
8. [8] Viver, T., Orellana, L. H., Hatt, J. K., Urdiain, M., Díaz, S., Richter, M. & Rosselló‐Móra, R. (2017). The low diverse gastric microbiome of the jellyfish Cotylorhiza tuberculata is dominated by four novel taxa. Environmental microbiology, 19(8), 3039-3058. https://doi.org/10.1111/1462-2920.13763

9. [9] Bayha, K.M., Dawson, M.N., Collins, A.G. & Barbeitos, M.S., Haddock, S.H. (2010). Evolutionary relationships among scyphozoan jellyfish families based on complete taxon sampling and phylogenetic analyses of 18S and 28S ribosomal DNA. Integrative and Comparative Biology, 50(3):436-55. https://doi.org/10.1093/icb/icq074
10. [10] Dawson, M.N. & Jacobs, D.K. (2001). Molecular evidence for cryptic species of Aurelia aurita (Cnidaria, Scyphozoa), The Biological Bulletin, 200, 92-96. https://doi.org/10.2307/1543089
11. [11] Koosha M, Oshaghi M.A., Sedaghat M. M., Vatandoost H., Azari-Hamidian S., Abai M.R., Hanafi-Bojd A. A. & Mohtarami F. (2017). Sequence Analysis of mtDNA COI Barcode Region Revealed Three Haplotypes Within Culex pipiens Assemblage, Experimental Parasitology, 181, 102-110. https://doi.org/10.1016/j.exppara.2017.08.003
12. [12] Kumar S., Stecher G. &Tamura K. (2016). Mega 7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870-1874. https://doi.org/10.1093/molbev/msw054
13. [13] Martens, J. (1978). Spinnentiere, Arachnida: Weberknechte, Opiliones. In: Die Tierwelt Deutschlands. Gustav Fischer Verlag Jena. 64: 1-464.
14. [14] Kurt, K. (2014). Updated checklist of harvestmen (Arachnida: Opiliones) in Turkey. Archives of biological sciences, 66 (4), 1617-1631. https://doi.org/10.2298/ABS1404617K
15. [15] Yigit, N., Bayram, A., Çorak, İ. & Danisman, T. (2007). External morphology of the male harvestman Phalangium opilio (Arachnida: Opiliones). Annals of the Entomological Society of America, 100 (4), 574-581. https://doi.org/10.1603/0013-8746(2007)100[574:EMOTMH]2.0.CO;2
16. [16] Bayram, A. & Çorak, İ. (2007). A new record for the harvest spider fauna of Turkey: Dicranolasma giljarovi Silhavy, 1966 (Opilionida, Dicranolasmatidae). Turkish Journal of Zoology, 31(1), 9-12.
17. [17] Kurt, K., Kurt, P. & Yağmur, E.A. (2025). Morphological and molecular evidence for a new species of the genus Dicranolasma Sørensen 1873 Opiliones Dicranolasmatidae from Türkiye. Zootaxa, 5691(1), 93–102. https://doi.org/10.11646/zootaxa.5691.1.4
18. [18] URL1-https://www.ncbi.nlm.nih.gov/nuccore/MN097806.1 Erişim tarihi: 23.11.2025
19. [19] URL2- https://www.https://www.ncbi.nlm.nih.gov/nuccore/MN192912.1 Erişim tarihi: 23.11.2025
20. [20] Giribet, G., Rambla, M., Carranza, S., Baguñà, J., Riutort, M. & Ribera, C. (1999). Phylogeny of the arachnid order Opiliones (Arthropoda) inferred from a combined approach of complete 18S and partial 28S ribosomal DNA sequences and morphology. Molecular Phylogenetics and Evolution, 11(2):296-307. https://doi/10.1006/mpev.1998.0583.
21. [21] Hwang, UW. & Kim, W. (1999). General properties and phylogenetic utilities of nuclear ribosomal DNA and mitochondrial DNA commonly used in molecular systematics. The Korean Journal of Parasitology, 37(4): 215–228. https://doi/10.3347/kjp.1999.37.4.215
22. [22] Hickerson, M.J., Meyer, C. & Moritz, C. (2006). DNA Barcoding Will Often Fail to Discover New Animal Species Over Broad Parameter Space. Systema Biology, 1(55)729-739, VDOI:10.1080/10635150600969898.
23. [23] Chen, S. & Shih, T. (2018). A Review of the Harvestman Genus Metadentobunus (Opiliones: Sclerosomatidae: Gagrellinae) With a Description of A New Species From Taiwan, Zoological Studies, 57. https://doi/10.6620/ZS.2018.57-57
24. [24] Ortiz, D. & Francke, O.F. (2016). Two DNA barcodes and morphology for multi-method species delimitation in Bonnetina tarantulas (Araneae: Theraphosidae). Molecular Phylogenetics and Evolution, 101:176-193. https://doi/10.1016/j.ympev.2016.05.003
25. [25] Roy, B.C., Estrada-Peña, A., Krücken, J., Rehman, A. & Nijhof, A.M. (2018). Morphological and Phylogenetic Analyses of Rhipicephalus Microplus Ticks from Bangladesh, Pakistan and Myanmar. Ticks and Tick-Borne Diseases, 9(5), 1069-1079. https://doi/10.1016/j.ttbdis.2018.03.035