Metaphalangium Roewer, 1911 cinsinin ilk moleküler verileri ve filogenetik ilişkileri

Abstract

Taksonomik ilişkilerin belirlenmesinde ve bazı durumlarda morfolojik veriler tek başına yetersiz kalabilir. Bu nedenle, moleküler verilerin morfolojik kanıtlarla birleştirilmesi, hem yeni taksonların tanımlanmasında hem de filogenetik ilişkilerin aydınlatılmasında çalışmaların güvenilirliğini artırmaktadır. Metaphalangium Roewer, 1911 bir otbiçen cinsi olup, bugüne kadar bu cins veya tür için herhangi bir moleküler veri bildirilmemiştir. Bu çalışmada, Kıbrıs'tan toplanan Metaphalangium cirtanum (Koch, 1839) türünün mitokondriyal genomunun 16S rRNA gen bölgesi sekanslanarak yaklaşık 457 bp uzunluğunda bir fragman elde edilmiştir. Bu moleküler verilere dayanarak, türün ve cinsinin filogenetik ilişkileri, Bayesçi Çıkarım (BI) ve Maksimum Olasılık (ML) analizleri kullanılarak burada ilk kez ortaya çıkarılmıştır. Amaç: Bu çalışma, daha önce moleküler verisi bulunmayan Metaphalangium cinsi ve M. cirtanum türü için mitokondriyal 16S rRNA gen bölgesine ait ilk moleküler verileri sunmayı ve bu veriler yardımıyla cinsin filogenetik ilişkilerini ortaya koymayı amaçlamaktadır. Metod: Kıbrıs’tan toplanan Metaphalangium cirtanum türüne ait mitokondriyal 16S rRNA gen bölgesi PCR yöntemi ile çoğaltılmış, elde edilen DNA dizileri analiz edilerek türler arasındaki genetik mesafeler hesaplanmış ve Maksimum olasılık (ML) ile Bayesian çıkarım (BI) yöntemleri kullanılarak filogenetik ağaçlar oluşturulmuştur. Bulgular: Analizler sonucunda, bu türün 16S rRNA gen bölgesine ait 457 bp uzunluğunda bir dizi elde edilmiştir. Elde edilen diziler yardımıyla gerçekleştirilen Maximum Likelihood analizinde yüksek bootstrap destek değerleri, Bayesian çıkarım analizinde ise yüksek posterior olasılık değerleri elde edilmiştir. Her iki yönteme ait filogenetik ağaçların benzer topolojik yapı göstermesinden dolayı sonuçlar tek bir ağaç üzerinde birleştirilerek sunulmuştur. Sonuç: Bu çalışma ile Metaphalangium cirtanum türüne ait moleküler veriler ilk kez elde edilmiştir. Gelecekte, daha fazla gen bölgesinin kullanıldığı ve daha geniş bir taksonomik örneklemi kapsayan çalışmaların gerçekleştirilmesi, Metaphalangium cinsinin filogenetik ilişkilerinin ve sistematik konumunun daha net bir şekilde ortaya konulmasına katkı sağlayacaktır.

Keywords

• Kıbrıs
• Metaphalangium cirtanum
• otbiçen
• 16S rRNA

Supporting Institution

Herhangi bir fon alınmadı.

Ethical Statement

Bu çalışma etik kurul onayı gerektirmemektedir.

Thanks

Bu çalışmada kullanılan örnekleri toplayan Kadir Boğanç KUNT'a (Kıbrıs Yaban Hayatı Araştırma Enstitüsü, Taşkent, Girne, Kıbrıs) ve türleri tanımlayan Dr. Kemal KURT'a (Gümüşhane Üniversitesi, Türkiye) teşekkür ederim.

First molecular data and phylogenetic relationships of the harvestman genus Metaphalangium Roewer, 1911

Abstract

In determining taxonomic relationships, morphological data alone may be insufficient in some cases. Therefore, the integration of molecular data with morphological evidence enhances the reliability of studies, both in the identification of new taxa and in the elucidation of phylogenetic relationships. Metaphalangium Roewer, 1911 is a harvestman genus, and to date, no molecular data has been reported for this genus or species. This study investigated, the 16S rRNA gene region of the mitochondrial genome of Metaphalangium cirtanum (Koch, 1839), collected from Cyprus was sequenced, yielding an approximately 457 bp long fragment. Based on these molecular data, the phylogenetic relationships of the species and its genus are here inferred for the first time using Bayesian Inference (BI) and Maximum Likelihood (ML) analyses. Purpose: This study aims to present the first molecular data on the mitochondrial 16S rRNA gene region for the genus Metaphalangium and the species M. cirtanum, for which no molecular data previously existed, and to elucidate the phylogenetic relationships of the genus using these data. Method: 16S rRNA gene region of the species Metaphalangium cirtanum, collected from Cyprus, was amplified using the PCR method; the resulting DNA sequences were analyzed to calculate genetic distances between species, and phylogenetic trees were constructed using the Maximum Likelihood (ML) and Bayesian inference (BI) methods. Findings: As a result of the analyses, a 457-bp sequence from the 16S rRNA gene region of this species was obtained. In the Maximum Likelihood analysis conducted using the obtained sequences, high bootstrap support values were obtained, while high posterior probability values were obtained in the Bayesian inference analysis. Since the phylogenetic trees derived from both methods exhibited similar topological structures, the results were combined and presented on a single tree. Conclusion: In this study, molecular data for the species Metaphalangium cirtanum were obtained for the first time. In the future, conducting studies that utilize additional gene regions and encompass a broader taxonomic sample will contribute to a clearer understanding of the phylogenetic relationships and systematic position of the genus Metaphalangium.

Keywords

• Cyprus
• Harvestman
• Metaphalangium cirtanum
• 16S rRNA

Supporting Institution

No Funding Received

Ethical Statement

This study does not require ethical approval

Thanks

I would like to thank Dr. Kadir Boğanç KUNT (Cyprus Wildlife Research Institute, Taşkent, Girne, Cyprus) and Dr. Salih GÜCEL (Near East University, Cyprus) for collecting the specimens used in this study, and Dr. Kemal KURT (Gümüşhane University, Türkiye) for identifying the species.

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