Review of entomopathogenic microsporidia biodiversity in the Trabzon region, Turkey

Abstract

Review of Entomopathogenic Microsporidia Biodiversity in the Trabzon Region, Turkey Hilal YILDIRIM 1, Çağrı BEKİRCAN2 Onur TOSUN3 ORCID: 0000-0002-6072-5543;0000-0002-5968-7359;0000-0002-6763-5671 1 Department of the Crop and Animal Production, Espiye Vocational School, Giresun University, Giresun, Türkiye 2 Department of the Chemistry and Chemical Processing Technology, Maçka Vocational School, Karadeniz Technical University, Trabzon, Türkiye 3 Department of the Veterinary Medicine, Maçka Vocational School, Karadeniz Technical University, Trabzon, Türkiye Abstract Purpose: Entomopathogenic microsporidia members are obligate intracellular parasites. They play an effective role of in the biological control of insect pest. In resent years, there has been increasing interest in biological pest control using entomopathogenic microsporidia as an alternative to chemical control methods. Trabzon region has a rich ecosystem with its coastal areas, mountainous regions, high plateaus, forests, streams, and wildlife. The main factor contributing to biodiversity is the the structure of the ecosystem. This rich biodiversity, both flora and fauna, has need to documented the biodiversity of entomopathogenic microsporidia in the region. To date, there is no study has been conducted to list entomopathogenic microsporidia in Trabzon province. In this study, it was aimed to reveal microsporidia biodiversity that have been recorded from Trabzon region to date. Method: Recently published articles about entomopathogenic microsporidium in Turkey especially from Trabzon city and literature information were taken as basis in the preparation of this article Findings: To date, numerous microsporidia have been recorded from orders with high species diversity in the Trabzon region, such as Hymenoptera, Hemiptera, Lepidoptera, and Coleoptera. While some of these have been identified in beneficial insects, such as bees and predatory species, the majority have been recorded from insect pests of economically important plants. In addition, various studies have emphasized the effects of geographical structure and climatic conditions on biodiversity. In this context, the diversity of entomopathogenic microsporidia in the Trabzon region has been discussed by considering the topographical structure. Conclusion: To date, the entomopathogenic microspores recorded from both usefull and harmful insects in Trabzon provide evidence of the wide pathogen diversity in the region. At the same time, they indicate the significant potential for furter research in the area. Due to its geographical characteristics, Trabzon province still has unexplored areas in terms of entomopathogenic microsporidium diversity.Studies show that Trabzon is a very rich and virgin region in terms of entomopathogenic microsporidia.

Keywords

• Microsporidia
• Trabzon
• biodiversity

Türkiye’de Trabzon bölgesinin entomopatojenik mikrosporidium çeşitliliği

Abstract

Türkiye’de Trabzon bölgesinin entomopatojenik mikrosporidium çeşitliliği Özet Amaç: Entomopatojenik mikrosporidiya üyeleri, zorunlu hücre içi parazitlerdir. Zararlı böceklerle biyolojik mücadelede etkili bir rol oynarlar. Son yıllarda, kimyasal mücadele yöntemlerine alternatif olarak entomopatojenik mikrosporidiyaların kullanıldığı biyolojik mücadeleye olan ilgi giderek artmaktadır. Trabzon kıyı kesimi, dağlık alanlar, yüksek yaylalar, ormanlar, akarsular ve yaban hayatıyla zengin bir ekosisteme sahiptir. Biyoçeşitliliği oluşturan temel faktör ise Ekosistemin yapısıdır. Bitkisel ve hayvansal anlamda var olan bu zengin biyoçeşitlilik, bölgede entomopatojenik mikrosporidia biyoçeşitliliğinin de ortaya konulması ihtiyacını doğurmuştur. Bugüne kadar Trabzon ilinde entomopatojenik mikrosporidiaların listelenmesine yönelik bir çalışma bulunmamaktadır. Bu çalışmada, Trabzon bölgesinde bugüne kadar kaydedilen mikrosporidiya biyoçeşitliliğinin ortaya konulması amaçlanmıştır. Metod: Bu makalenin hazırlanmasında, Türkiye'de, özellikle Trabzon ilinden, entomopatojenik mikrosporidyumlar hakkında yakın zamanda yayınlanan makaleler ve litaratürdeki bilgiler esas alınmıştır. Bulgular: Bugüne kadar Trabzon bölgesinden Hymenoptera, Hemiptera, Lepidoptera ve Coleoptera gibi geniş tür sayısına sahip takımlardan birçok mikrospor kaydedilmiştir. Bunlardan bazıları arılar ve predatör böcekler gibi faydalı böceklerden kaydedilirken, çoğunluğunun ekonomik önemi olan bitkilerde zararlı olan böceklerden kaydedildiği görülmektedir. Ayrıca çeşitli çalışmalarda coğrafik yapı ve iklim şartlarının biyoçeşitlilik üzerindeki etkilerine vurgu yapılmıştır. Bu bağlamda Trabzon bölgesindeki entomopatojenik mikrosporidium çeşitliliği topoğrafik yapı göz önüne alınarak tartışılmıştır. Sonuç: Bugüne kadar Trabzon’dan hem yararlı hem zararlı böceklerden kaydedilen entomopatojenik mikrosporlar, bölgedeki geniş patojen çeşitliliğinin bir kanıtıdır. Aynı zaman da yapılacak yeni çalışmalar için bölgedeki potansiyelin bir göstergesidir. Trabzon ili coğrafyası nedeniyle entomopatojenik mikrospor çeşitliliği açısından hala keşfedilmemiş alanlara sahiptir.

Keywords

• microsporidia
• Trabzon
• biyoçeşitlilik

References

1. [1] Türkiye Orman Varlığı (2020). T. C. Tarım ve Orman Bakanlığı, Orman Genel Müdürlüğü, Orman idaresi ve planlama dairesi başkanlığı.
2. [2] Bekircan, Ç. & Tosun, O. (2021). A new microsporidian pathogen of the Mediterranean flour moth, Ephestiae kuehniella (Zeller) (Lepidoptera: Pyralidae). Biological Diversity and Conservation, 14, 277–285. https://doi.org/10.46309/biodicon.2021.966465
3. [3] Bekircan, Ç., Tosun, O. & Baki, H. (2018). Influence of climatic conditions on microsporidiosis that originated from Rugispora istanbulensis in Elm Leaf Beetle, Xanthogaleruca luteola Muller (Coleoptera: Chrysomelidae). Kastamonu Üniversitesi Orman Fakültesi Dergisi, 18, 230–235. https://doi.org/10.17475/kastorman.344845
4. [4] Yıldırım, H. (2021). The first record of Vairimorpha hostounsky sp. nov. Infection in the blue shieldbug, Zicrona caerulea Linnaeus, 1758 (Hemiptera: Pentatomidae). Microbial Pathogenesis, 155, 104883. https://doi.org/10.1016/j.micpath.2021.104883
5. [5] Baki, H. & Bekircan, Ç. (2018). A new microsporidium, Vairimorpha subcoccinellae n. sp. (Microsporidia: Burenellidae), isolated from Subcoccinella vigintiquatuorpunctata L. (Coleoptera: Coccinellidae). Journal of Invertebrate Pathology, 151, 182-190. https://doi.org/10.1016/j.jip.2017.12.004
6. [6] Hajek, A. E. & Delalibera, I. (2010). Fungal pathogens as classical biological controlagents against arthropods. Biol. Control, 55, 147–158. https://doi.org/10.1016/j.biocontrol.2010.07.003
7. [7] Yaman, M. & Güner, B. G. (2015). Nosema chaetocnemae Yaman & Radek, 2003 (Microspora)’ın iki farklı izolatının morfolojik ve enfeksiyon özellikleri açısından karşılaştırılması. Bitki Koruma Bülteni, 55(2): 123-130.
8. [8] Yıldırım, H. & Bekircan, Ç. (2020). Ultrastructural and molecular characterization of Nosema alticae sp. nov. (Microsporidia: Nosematidae), pathogen of the flea beetle, Altica hampei Allard, 1867 (Coleoptera: Chrysomelidae). Journal of Invertebrate Pathology, 170, 107302. https://doi.org/10.1016/j.jip.2019.107302

9. [9] Solter, L.F., Becnel, J. J. & David, H. O. (2012). Microsporidian entomopathogens. In Vega FE and Kaya HK (eds), Insect Pathology. London: Elsevier Inc., pp. 221–263.
10. [10] Slamovits, C. H., Williams, B. A. P. & Keeling, P.J. (2004). Transfer of Nosema locustae (Microsporidia) to Antonospora locustae n. comb. based on molecular and ultrastructural data. J. Eukaryot. Microbiol. 51, 207–213. https://doi.org/10.1111/j.1550-7408.2004.tb00547.x
11. [11] Roberts, L.S. & Janovy, J. (2009). Microsporidia and Myxozoa: parasites with polar filaments. Foundations of parasitology, eighth ed. The McGraw-Hill Companies Inc, pp. 183–194.
12. [12] Schenker, W., Maier, W. A. & Seitz, H. M. (1992). The efects of Nosema algerae on the development of Plasmodium yoelii nigeriensis in Anopheles stephensi. Parasitol Res., 78: 56-59. https://doi.org/10.1007/BF00936182
13. [13] Bass, L. K. & Armstrong, E. (1992). Nosema whitei: effects on oocyte development and maturation in Tribolium castaneum. J. Invertebr. Pathol., 59, 115–123. https://doi.org/10.1016/0022-2011(92)90021-U
14. [14] Windels, M. B., Chiang H. C. & Furgala B. (1976). Effects of Nosema pyrausta on pupa and adult stages of the european corn borer, Ostrinia nubilalis. J. Invertebr. Pathol., 27 (2), 239-242. https://doi.org/10.1016/0022-2011(76)90151-8
15. [15] Bekircan, Ç., Tosun, O. & Yildirim, H. (2025). Nosema canburensis sp. nov. (Microsporidia: Nosematidae): a novel microsporidium record infecting the Italian striped bug, Graphosoma italicum Müller (Hemiptera: Pentatomidae). Systematıc Parasitology, 102, 2. https://doi.org/10.1007/s11230-025-10220-4
16. [16] Bekircan, Ç., Tosun, O. & Yıldırım, H. (2023). A record of microsporidian pathogen of the European wasp, Vespula vulgaris Linnaeus, 1758 (Hymenoptera: Vespidae) in Turkey. Journal of Apitheraphi and Nature, 6(2), 30-40. https://doi.org/10.35206/jan.1362309
17. [17] Tosun, O. (2020). A new isolate of Nosema fumiferanae (Microsporidia: Nosematidae) from the date moth Apomyelois (Ectomyelois) ceratoniae, Zeller, 1839 (Lepidoptera: Pyralidae). Parasitology, 147(13), 1461-1468. https://doi.org/10.1017/S0031182020001481
18. [18] Bekircan, Ç. (2020). Assignment of Vairimorpha leptinotarsae comb. nov. on the basis of molecular characterization of Nosema leptinotarsae Lipa, 1968 (Microsporidia: Nosematidae). Parasitology, 147, 1019–1025. https://doi.org/10.1017/S0031182020000669
19. [19] Yaman M., Güngör F. P., Güner B. G., Radek R. & Linde A. (2016). First report and spore ultrastructure of Vairimorpha plodiae (Opisthokonta: Microspora) from Plodia interpunctella (Lepidoptera: Pyralidae) in Turkey. Acta Parasitology, 61, 228–231. https://doi.org/10.1515/ap-2016-0032
20. [20] Yaman, M., Bekircan, C., Radek, R. & Linde A. (2014). Nosema pieriae sp n. (Microsporida, Nosematidae): A new microsporidian pathogen of the cabbage butterfly Pieris brassicae L. (Lepidoptera: Pieridae). Acta Protozoologica , 53 (2), 223-232. https://doi.org/10.4467/16890027AP.14.019.1600
21. [21] Yaman, M., Radek, R., Linde, A., Özcan, N. & Lipa, J. J. (2011). Ultrastructure, characteristic features and occurrence of Nosema leptinotarsae Lipa 1968, a microsporidian pathogen of Leptinotarsa decemlineata Coleoptera, Chrysomelidae). Acta Parasitologica, 56, 1-7. https://doi.org/10.2478/s11686-011-0009-y
22. [22] Yaman, M. (2008). First results on the distribution of Nosema chaetocnemae (Microspora) in the populations of Chaetocnema tibialis (Coleoptera, Chrysomelidae) in Turkey. Türkiye Parazitol Derg, 32, 94-98.
23. [23] Tosun, O. & Yaman, M. (2016). The effects of temperature and humidity around the beehives on the distribution of Nosema ceranae, and also geographical and seasonal activity of the infection in the Eastern Black Sea Region of Turkey. Journal of Environmental Science and Engineering B. 5, 513-522. doi:10.17265/2162-5263/2016.11.001
24. [24] Yaman, M., Radek, R., Weiser, J. & Aydın, Ç. (2010). A microsporidian pathogen of the predatory beetle Rhizophagus grandis (Coleoptera: Rhizophagidae). Folia Parasitologica, 57, 233-236. https://doi.org/10.14411/fp.2010.031
25. [25] Singh, R. N., Daniel, A. G. K., Sindaggi, S. S., & Kamble, C. K. (2007). Microsporidians infecting silkworm, Bombyx mori. Sericologia, 47, 1-16.
26. [26] Yaman, M. & Radek, R. (2003). Nosema chaetocnemae sp. n., a microsporidian (Microspora, Nosematidae) parasite of Chaetocnema tibialis (Chrysomelidae, Coleoptera). Acta Protozoologica, 42, 231-237.
27. [27] Lewis, L. C., Bruck, D. J., Prasifka, J., R. & Raun, E. S. (2009). Nosema pyrausta: Its biology, history, and potential role in a landscape of transgenic insecticidal crops. Biological Control, 48,223–231 https://doi.org/10.1016/j.biocontrol.2008.10.009
28. [28] Aydın, L., Çakmak, İ., Güleğen, E. & Wells, H. (2005). Honeybee Nosema disease in the Republic of Turkey. Journal of Apicultural Research, 44(4), 196-197. https://doi.org/10.1080/00218839.2005.11101179
29. [29] Huang, W. F., Jiang, J. H., Chen, Y. W. & Wang, C. H. (2007). A Nosema ceranae isolate from the honey bee Apis mellifera, Apidologie, 38, 30-37. https://doi.org/10.1051/apido:2006054
30. [30] Fries, I. (2010). Nosema ceranae in european honey bees (Apis mellifera). Journal of Invertebrate Pathology, 103, 73–79. https://doi.org/10.1016/j.jip.2009.06.017
31. [31] Malone, L., A., Gatehouse, H., S., & Tregidga, E. (2001). Effects of time, temperature and honey on Nosema apis (Microsporidia: Nosematidae), a Parasite of the Honeybee, Apis mellifera (Hymenoptera: Apidae). Journal of Invertebrate Pathology, 77, 258–268 https://doi.org/10.1006/jipa.2001.5028
32. [32] Martín-Hernández, R., Meana, A., García-Palencıa, P., Marín, P., Botías, C., Garrıdo- Baılón, E., Barrıos, L. & Hıges, M. (2009). Effect of temperature on the biotic potential of honey bee microsporidia. Applied and Environmental Microbiology, 75, 2554-2557. https://doi.org/10.1128/AEM.02908-08
33. [33] De la Rocque, S. J., Rioux, A. & Slingenbergh, J. (2008). Climate change: effects on animal disease systems and implications for surveillance and control. Rev. Sci. Tech. Off. Int. Epizoot, 27, 339–354. https://doi.org/10.20506/rst.27.2.1808
34. [34] Dussaubat, C., Sagastume, S., Gómez-Moracho, T., Botías, C., García-Palencia, P., Martín-Hernández, R., Le Conte, Y. & Higes, M. (2013). Comparative study of Nosema ceranae (Microsporidia) isolates from two different geographic origins. Vet. Microbiol, 162(2-4), 670-678. https://doi.org/10.1016/j.vetmic.2012.09.012
35. [35] Yaman, M. (2017). Entomopathogen diversity of Kastamonu Region. Kastamonu Univ. Journal of Forestry Faculty, 17(3), 368-372. Doi: 10.17475/kastorman.293151