Determination of Growth Inhibitory and Apoptotic Effects of Protocatechuic Acid on Prostate Carcinoma Cells

Abstract

Protocatechuic acid PCA is a phenolic acid and widely found spread throughout in many plants as aromatic secondary metabolites. Previous studies have shown different biological and pharmacological activities of PCA, as well as, suppression of proliferation of cancer cells. Prostate cancer is one of the most common cancer types in men and the treatment is very limited. In this study, we determined the cytotoxic and apoptotic effects of PCA on human prostate cancer DU145 cells. The cytotoxic effect of PCA 0.5 to 3.5 mM was examined in cells for 24 and 48 hours by MTT and Neutral Red NR assay. All statistical analyses were performed using one-way analysis of variance ANOVA and followed up by Tukey’s multiple comparison tests. Morphological changes in cells were evaluated by inverted microscope. Apoptotic cell death was assessed in cells treated with 1 and 1.5 mM PCA by DAPI staining. The cell viability started to decrease at 1 mM p

Keywords

• protocatechuic acid,prostate carcinoma,cytotoxicity,DAPI,apoptosis

Prostat Kanseri Hücreleri Üzerinde Protokatekuik Asitin Büyümeyi Önleyici ve Apoptoz İndükleyici Etkilerinin Belirlenmesi

Abstract

Protokatekuik asit PCA ; fenolik bir asittir ve pek çok bitkide yaygın olarak bulunan aromatik sekonder bir metabolitdir. Önceki araştırmalar, PCA'nın farklı biyolojik ve farmakolojik aktiviteleri yanında özellikle kanser hücrelerinin çoğalmasını da baskıladığını göstermiştir. Prostat kanseri, erkeklerde görülme oranı yüksek kanser çeşitlerinden birisidir ve tedavisi oldukça sınırlıdır. Çalışmamızda, PCA’nın insan prostat kanseri DU145 hücreleri üzerindeki sitotoksik, morfolojik ve apoptotik etkileri araştırıldı. PCA dozlarının 0,5 ile 3,5 mM 24 ve 48 saatteki sitotoksik etkileri MTT ve Nötral Kırmızısı NR yöntemleriyle araştırıldı. Deney grupları arasındaki farklılıklar, SPSS programında, tek yönlü varyans analizi ve ardından Tukey’in çok yönlü karşılaştırma yöntemiyle değerlendirildi. Hücrelerdeki morfolojik değişiklikler ters ışık mikroskobu ile incelendi. Ayrıca 1 ve 1,5 mM PCA ile muamele edilen hücrelerdeki apoptotik hücre ölümü, DAPI boyaması ile araştırıldı. PCA’nın hücrelerindeki çoğalmayı baskılayıcı etkisinin 24 saatte 1 mM p

Keywords

• protokatekuik asit,prostat kanseri,sitotoksisite,DAPI,apoptoz

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