The effect of Cuscuta babylonica Aucher Cuscuta parasitism on the phenolic contents of Alhagi maurorum

Abstract

Parasitic plants are restrict the lives of earth plants. Cuscuta species are holoparasitic plants without leaves or roots. Water and nutrients are transferred through the haustorium from the vascular system of the host into the parasite. Parasitic plants are affected by host plant physiology because of similar hormonal pathways between parasite and host plants. Host plant secretes various phenolic compounds, as a response to parasitic plant. Alhagi, a plant genus from family Fabaceae, is widely distributed in many countries of Asia, Australia ve Europe. Abroad range of biological activities such as antioxidant, cardiovascular, antiulcer, antidiarrheal, antipyretic, anti-inflammatory, anti-rheumatic, antibacterial ve antifungal have been ascribed to different parts of Alhagi. In this study it is determined that the infection of Cuscuta babylonica Aucher Cuscutaceae on the phenolic compound changes of Alhagi maurorum by LC-MS/MS anlysis. As our results, it is revealed that the Cuscuta infection lead to an important increasing on the amount of chlorogenic acid 67.64 fold , apigenin 23.18 fold and quercetin 6.75 fold , besides improved the amount of hiperoside 3.94 fold , naringenin 2.92 , p-cumaric acid 2.28 fold , quinic acid 2.08 fold , coumarin 1.74 fold , trcafeic acid 1.61 fold , salicylic acid 1.40 fold , 4-OH benzoic acid 1.36 fold , malic acid 1.109 fold ve hesperidin 1.08 fold . The amount of kaemferol 5.04 fold , gallic acid 2.58 fold , vanillin 1.54 fold , tr-akonitic acid 2.41 fold , tannic acid 2.11 fold , rosmarinic acid 1.88 fold , and rutin 1.044 fold decreased when it is observed an significant decline the amount of protocatecholic acid 36.34 fold . Hesperetin is found only infected samples

Keywords

• Cuscuta babylonica Aucher,Alhagi maurorum,phenolic compound,LC/MS-MS

Cuscuta babylonica Aucher Küsküt parazitliğinin Alhagi maurorum ’un fenolik bileşikleri üzerine etkisi

Abstract

Parazit bitkiler, yeryüzünde konuk oldukları bitkilerin yaşamlarını sınırlandıran türlerdir. Küsküt türleri, yaprak ve kökleri olmayan tam parazit bitkilerdir. Bu bitkiler, su ve besin elementlerini özelleşmiş beslenme yapıları olan hostoryumları aracılığıyla diğer bitkilerden elde ederler. Parazit bitkiler konukçularıyla benzer hormonal mekanizmalar içerdikleri için hem onları etkilerler hem de onlardan etkilenirler. Parazit bitkilerin saldırısına uğrayan konukçu bitki, en etkin savunma maddeleri olan çeşitli fenolik bileşikler sentezler. Alhagi, Fabaceae familyasından Asya, Avusturalya ve Avrupa’nın çoğu ülkesinde geniş dağılım gösteren bir bitki cinsidir. Alhagi’nin farklı kısımları, antioksidant, kardiyovasküler, antiülser, diare önleyici, ateş düşürücü, anti romatizmal, antibakteriyel, antifungal gibi geniş bir biyolojik aktiviteye sahiptir. Bu çalışmada patojen Cuscuta babylonica Aucher Cuscutaceae enfeksiyonunun Alhagi maurorum bitkisinin fenolik bileşik içeriğinde meydana getirdiği değişiklikler LC-MS/MS analizleri kullanılarak incelenmiştir. Analizler sonucunda, küsküt enfeksiyonunun klorogenik asit 67.64 kat , apigenin 23.18 kat ve kuersetin 6.75 kat içeriklerinde önemli bir artışa neden olurken, bunun yanında hiperosid 3.94 kat , naringenin 2.92 kat , p-kumarik asit 2.28 kat , kuinik asit 2.08 kat , kumarin 1.74 kat , tr- caffeik asit 1.61 kat , salisilik asit 1.40 kat , 4-OH benzoik asit 1.36 kat , malik asit 1.109 kat ve hesperidin 1.08 kat bileşiklerinde de artış olduğu ortaya çıkarılmıştır. Protokateşuik asit miktarında 36.34 kat gibi kaydadeğer bir düşüş gözlenirken, yine kaemferol 5.04 kat , gallik asit 2.58 kat , vanillin 1.54 kat , tr-akonitik asit 2.41 kat , tannik asit 2.11 kat , rosmarinik asit 1.88 kat , rutin 1.044 kat gibi bileşiklerin miktarında da düşüşe neden olmuştur. Hesperetin 0.268 ise sadece, enfekte olmuş bitkilerde tesbit edilmiştir. Enfekte olmadan önce ve enfeksiyon sonrası yapılan analiz sonuçları küsküt enfeksiyonunun Alhagi bitkisinin fenolik bileşen içeriğinde önemli değişimlere neden olduğunu ortaya koymuştur

Keywords

• Cuscuta babylonica Aucher,Alhagi maurorum,fenolik bileşikler,LC/MS-MS

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