Brezilya Barbatimão ağacından izole edilen endofitik fungusların uçucu metabolitleri

Abstract

Endofitik funguslar bitkilerle ortaklaşa bir yaşam sürdürmekle birlikte, onların biyotik ve abiyotik stres koşullarına karşı dirençlerini arttırmaktadırlar. Endofitik fungusların biyoçeşitliliği çok fazladır ve birçok biyoaktif bileşik sentezleyebilirler. Bu basit yapılı doğal moleküller ilaç, gıda ve parfümeri endüstrisinin büyük ilgisini çekmektedir. Çalışmamızda Brezilya’nın yerel bir bitkisi olan Stryphodendron adstringens’in yaprak ve gövde kabuklarından 5 farklı endofitik fungus izole edilerek moleküler metotlar ile tanımlanmışlardır. Funguslar uygun sıvı besi ortamı içerisinde kültüre alınarak, ürettikleri uçucu madde profili tepe boşluğu-katı faz mikro-ekstraksiyon ve gaz kromatografisi/kütle spektrometresi GK/KS kullanılarak, ortaya konmuştur. İzole edilen fungusların terpen yapısında mono- ve seskiterpenler ile hidrokarbon yapısında organik uçucu bileşikler ürettikleri saptanmıştır

Keywords

• endofitler,tepe boşluğu-katı faz mikro-ekstraksiyonu,GK/KS,uçucu organik bileşikler

Volatile metabolites of endophytic fungi isolated from Brazilian Barbatimão tree

Abstract

Entophytic fungi live in a symbiotic association with higher plants and may improve the host resistance to biotic and/or abiotic stresses. Endophytes have a very rich biodiversity and they can synthesize so many bioactive metabolites. These simple natural molecules are challenging potential resources for the pharmaceutical, food and fragrance industries. In the present study five endophytic fungi were isolated from barks and leaves of Brazilian native tree Stryphodendron adstringens and identified by molecular methods. The fungi were cultured in liquid media and the volatile compounds profile were determined by using headspace solid-phase micro-extraction HS-SPME and gas chromatography/mass spectrometry GC/MS techniques. Hydrocarbones and the terpenic compounds mono- and sesquiterpenes were detected as the main volatiles of the isolated fungus

Keywords

• endophytes,headspace-solid phase micro-extraction,GC/MS,volatile organic compounds

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