Distribution patterns and ISSR PCR optimisation of invasive plant Eichhornia crassipes in Asi River/Turkey

Abstract

Eichhornia crassipes Mart. Solms Pontederiaceae is a perennial aquatic invasive weed throughout the tropical and subtropical regions of the world. Recently, this species was detected in Asi River, Hatay. Therefore, knowledge on its population structure, size and density is very important to understand the current status of this species in Turkey. In this study, we have determined the distribution pattern of E. crassipes. After monitoring Asi River, spanning Syrian Border to Mediterranian Sea, we have detected three main population located at Reyhanlı, Antakya and Samandağ. Population size of E. crassipes varied between 4900-13600. ISSR-PCR protocol was also optimized for E. crassipes based on concentrations of MgCl2, primer, dNTP and template DNA. Among 45 ISSR primers tested, 20 of them have produced satisfactory results. Study results suggested that distribution of E. crassipes in Turkey is not negligible and populations must be monitored periodically. In addition, the optimized ISSR-PCR procedure can be used to study the genetic structure of E. crassipes populations naturally grown in Turkey

Keywords

• Asi River,Eichhornia crassipes,ınvasive species,ISSR,Turkey

İstilacı Eichhornia crassipes bitkisinin Türkiye’de Asi Nehrinde dağılımı ve ISSR PCR optimizasyonu

Abstract

Eichhornia crassipes Mart. Solms Pontederiaceae dünyada tropik ve sub-tropik bölgelerde yayılış gösteren çok yıllık istilacı bir sulak alan bitkisidir. Bu bitki türü yakın zamanlarda Hatay yöresinde Asi Nehrinde tespit edilmiştir. Bu nedenle bu türün popülasyon yapısı, büyüklüğü, yoğunluğunun belirlenmesi Türkiye’deki güncel durumunun anlaşılması açısından oldukça önemlidir. Bu çalışmada E. crassipes’in Asi Nehri üzerindeki dağılım alanları tespit edilmiştir. Nehrin Suriye sınırı ve Akdeniz arasında kalan kısmı tarandığında Reyhanlı, Antakya ve Samandağ’da üç ana popülasyon tespit edilmiştir. Popülasyon büyüklükleri 4900-13600 arasında değişmektedir. Bunun yanında, E. crassipes için farklı MgCl2, primer, dNTP ve kalıp DNA konsantrasyonları temel alınarak ISSR-PCR protokolü optimize edilmiştir. Denenen 45 ISSR primerden 20’si kabul edilebilir sonuçlar vermiştir. Çalışma sonuçlarımız, E. crassippes’in Türkiye’deki dağılımının göz ardı edilemez seviyede olduğunu ve populasyonların düzenli olarak monitörlenmesi gerektiğini göstermektedir. Ek olarak, optimize edilen ISSR-PCR prosedürü Türkiye’de doğal yayılış gösteren E. crassipes populasyonlarının genetik yapılarının çalışılmasında kullanılabilecektir

Keywords

• Eichhornia crassipes,istilacı tür,Asi Nehri,ISSR,Türkiye

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