The effect of magnetic field application on chemical composition in Fagus orientalis Lipsky. seed

Abstract

Previous studies have shown that magnetic field MF can affect the germination process in some seeds of agricultural crops and influence on some seed characteristics. Therefore, in the present study, the effects of MF applications on the seed of Fagus orientalis were investigated. The unstratified seed were exposed to different treatment periods as 20, 60 and 120 min. and varied MF intensities as 200 and 400 mT. Accordingly, total protein, glucose, fructose, sucrose, starch content and α-amylase activity level, in the seeds were investigated. MF applications had affected total protein, α-amilaz activitiey level, glucose, fructose, sucrose, and starch contents at significant levels. MF had negative effects on soluble protein content and α-amylase activity level in the seeds. Soluble starch content increased as the MF treatment progressed, except the treatment of 200 mT at 20 min. as well control treatment

Keywords

• magnetic field,germination,Oriental beech,seed,strees factors

Magnetik alan uygulamalarının doğu kayını Fagus orientalis Lipsky. tohumunun kimyasal içeriğine etkisi

Abstract

Önceki çalışmalar magnetik alanın, bazı tarımsal ürün tohumlarının çimlenme sürecini etkileyebildiğini ve bazı tohum karakterleri üzerine etkileri olduğunu göstermiştir. Bu sebeple bu çalışmada; magnetik alan uygulamalarının doğu kayını tohumları üzerine etkileri araştırılmıştır. Katlama ön işlemine tabi tutulmayan tohumlar; farklı sürelerde 20, 60, 120 dakika ve 200 ile 400 mT yoğunluktaki magnetik alan uygulamasına maruz bırakılmıştır. Magnetik alan uygulamasına maruz bırakılan tohumlarda; toplam protein, glukoz, fruktoz, sukroz, nişasta içeriği ve α-amilaz akitivite seviyesi analiz edilmiştir. Magnetik alan uygulamaları, toplam protein, α-amilaz akitivite seviyesi, glukoz, fruktoz, sukroz ve nişasta içeriğini önemli ölçüde etkilemiştir. Magnetik alan uygulaması, tohumdaki çözünebilir protein içeriği ve α-amilaz akitivite seviyesini negatif etkilemiştir. 200 mT yoğunluk ve 20 dakika süreli magnetik alan uygulaması hariç, diğer magnetik alan işlemlerinde çözünebilir nişasta içeriği artmıştır

Keywords

• magnetik alan,çimlenme fizyolojisi,Doğu kayını,tohum,stres faktörleri

References

1. Agustrina, R., T. T. Handayani, S. Wahyuningsih, dan O. Prasetya. 2012. Pertumbuhan Tanaman Tomat (Lycopersicum esculentum Mill.) di Bawah Perlakuan Medan Magnet 0,2 mT. In the Proceedings of the 2012. SNSMAIP III, pp: 277-281.
2. Ak, A., Yücel, E., Ayan, S. 2012. Relationship between Seed Germination and Catalase Enzyme Activity of Abies taxa from Turkey, Kastamonu Univ., Journal of Forestry Faculty, 12 (3) Special Issue; 185-188.
3. Aladjadjiyan, A., Teodora, Y. 2003. Influence of stationary magnetic field on the early stages of the development of tobacco seeds (Nicotiana tabacum L.). Journal Central Europian Agriculture. Vol. 4. No. 2. p131-137.
4. Amaya, J.M., Carbonell, M.V., Martinez, E. & Raya, A. 1996. Effects of stationary magnetic fields on germination and growth of seeds. Hort. Abst. 68, 1363.
5. Anonim, 2006. Orman Varlığımız, Çevre ve Orman Bakanlığı, Orman Genel Müdürlüğü, OGM Matbaası, Ankara, Mart 2006, 152 pp.
6. Arenas-Huertero, F., Arroyo, A., Zhou, L., Sheen, J., León, P. 2000. Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar, Genes Dev. 2000 Aug 15; 14(16): 2085–2096.
7. Atak, C., Celik, O., Olgun A., Alikamanoglu, S., Rzakoulieva, A. 2007. Effect of Agnetic Field on Peroxidase Activities of Soybean Tissue Culture. Bıotechnol. & Biotechnol. EQ. 21 (2): 166-171.
8. Atalay, İ. 1992. Kayın (Fagus orientalis Lipsky.) Ormanlarının Ekolojisi ve Tohum Transferi Yönünden Bölgelere Ayrılması. Orman Bakanlığı, Orman Ağaçları ve Tohumları Islah Araştırma Müdürlüğü, Yayın No: 5, Ankara, 209 s.

9. Atay, İ. 1987. Doğal Gençleştirme Yöntemleri I-II, İ.Ü Fen Bilimleri Enstitüsü, İ.Ü Yayın No: 3461, F.B.E Yayın No: 1, İstanbul, 290 s.
10. Ayaz, F. A., Glew, R. H., Turna, I., Güney, D., Chuang, L. T., Chang, Y.C., Andrews, R., Power, L., Presley, J., Torun, H., Sahin, N. 2011. Fagus orientalis (Oriental beechnut) seeds are a good source of essential fatty acids, amino acids and minerals. Food 5: 48–51.
11. Barker, L., Kuehn, G., Weise, A., Schulz, A., Gebhart, C., Hirner, B., Hellman, H., Schulze, W., Ward, J. M. and Frommer, W. B. 2000. SUT2, a putative sucrose sensor in sieve elements. Plant Cell., 12:1153-1164.
12. Bewley, J.D. 1997. Seed germination and dormancy. Plant Cell, 9,1055±1066.
13. Bewley, J.D., Black, M. 1994. Seeds: Physiology of Development and Germination. 2nd edn. New York, NY:Plenum.
14. Bewley, J.D. 2001. Seed Germination and Reserve Mobilization. J Derek Bewley, University of Guelph, Guelph, Ontario, Canada. Encyclopedia of Life Sciences & 2001 Nature Publishing Group / www.els.net.
15. Bradford, M.M. 1976. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72: 248-254, doi:10.1016/0003-2697(76) 90527-3.
16. Criveanu, H.R., Taralunga, G. 2006. Influence of magnetic fields of variable intensity on behaviour of some medicinal plants. Journal of Central European Agricultura. Vol 7 (4): 643-648.
17. Danilov, V., Bas¸ T., Eltez, M. & Rizakulyeva, A. 1994. Artificial magnetic field effects on yield and quality of tomatoes. Acta Hort.366, 279-285.
18. De Souza, A., Garcí, D., Sueiro, L., Gilart, F., Porras, E., Licea, L. 2006. Pre-sowing magnetic treatments of tomato seeds increase the growth and yield of plants. Bioelectromag 27:247-257.
19. De Souza, A., D. García, L. Sueiro, L. Licea, Porras, E. 2005. Pre-sowing magnetic treatment of tomato Seeds: effects on the growth and yield of plants cultivated late in the season. Spanish Journal of Agricultural Research. 3(1), 113-122.
20. Debeaujon, I., Koornneef, M. 2000. Gibberellin requirement for Arabidopsis seed germination is determined both by testa characteristics and by embryonic abscisic acid. Plant Physiol. 122, 415±424.
21. Dekkers, B. J. W., Schuurmans, A. M. J., Smeekens, S. C. M. 2004. Glucose delays seed germination in Arabidopsis thaliana. Planta V218:579-588.
22. Dekkers, B.J. W., Smeekens, S. 2007. Sugar and abscisic acid regulation of germination and transition to seedling growth. In: Bradford K, Nonogaki H (eds) Seed development, dormancy and germination. Blackwell Publishing, Oxford, UK.
23. Dennis, D. T., and Blakeley, S. D. 2000. Carbohydrate metabolism. In Biochemistry and Moleculer Biology of Plants, B. Buchanan, Wgruis-sem and R Jones (eds) American Society of Plant Physiologist, Rockville MD, PP:630-674.
24. Dhawi, F., Al-Khayri, J.M., & Hassan, E. 2009. Static magnetic field influence on elements composition in date palm (Phoenix dactylifera L.). Res J Agric Biol Sci 5,161-166.
25. Dornbos, D. L., Mullen, R. E. 1992. Soybean seed protein and oil concentration and fatty-acid composition adjustments by drought and temperature. J. Am. Oil Chem. Soc. 69, 228–231.
26. Dunlop, D.W., Schmidt, B.L. 1965. Biomagnetics II. Anomalies found in the root of Allium cepa L. Hort. Abst. 36, 563.
27. Ertekin, M., Kırdar, E., Ayan, S. 2015. Effects of tree ages, exposures and elevations on some seed characteristics of Oriental beech (Fagus orientalis Lipsky.). SEEFOR - South-east European Forestry, 6 (1) 15-23.
28. Eşitken, A. 2002. Serada yetiştirilen çilekte manyetik alan uygulamasının etkileri, Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 34 (1): 25-27.
29. Eşitken, P.E. 2006. Elektromagnetik Alanın Lens Culinaris medik (Mercimek) Üzerinde Sitotoksik Etkileri, Marmara Üniversitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi.
30. Finkelstein, R.R., Lynch, T.J. 2000. Abscisic acid inhibition of radicle emergence but not seedling growth is suppressed by sugars. Plant Physiol 122:1179-1186.
31. Fincher, G.B. 1989. Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains. Annu Rev Plant Physiol Plant Mol Biol 40: 305-346.
32. Florez, M., Carbonell, M.V., Martinez, E. 2007. Exposure of maize seeds to stationary magnetic fields: Effects on germination and early growth. Environ Exp Botany 59:68-75.
33. Francisco Arenas-Huertero, A. A., Zhou, L., Sheen, J., León, P. 2000. Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar. Genes Dev, 14 (16), 2085-2096.
34. Garcia, F., Arze, L. I. 2001. Influence of a stationary magnetic field on water relations in lettuce seeds. Part I: Theoretical considerations, Bioelectromagnetics, Vol. 22, No. 8, pp. 589-595.
35. Garcia, I.S., Souza, A., Barbedo, C.J., Dietrich, S.M., Figueiredo-Ribeiro, R.C.L. 2006. Changes in soluble carbohydrates during storage of Caesalpinia echinata LAM. (Brazilwood) seeds, an endangered leguminous tree from the Brazilian Atlantic Forest. Braz. J. Biol. 66: 739-745.
36. Garciarrubio, A., Legaria, J.P., Covarrubias, A.A. 1997. Abscisic acid inhibits germination of mature Arabidopsis seeds by limiting the availability of energy and nutrients. Planta 203: 182–187.
37. Geigenberger, P., Kolbe, A., Tiessen, A. 2005. Redox regulation of carbon storage and partitioning in response to light and sugars. J Exp Bot, 56: 1469-1479.
38. Gezer, A., Yücedağ, C. 2006. Forest tree seeds and techniques of seedling propagation from seed originated. Suleyman Demirel University, Faculty of Forestry, Pub. No: 56, Isparta, Turkey, 140 p.
39. Gibson, S.I. 2005. Control of plant development and gene expression by sugar signaling. Curr. Opin. Plant Biol. 8:93 – 102 .
40. Guglielminetti, L., Busilacchi, H.A., Alpi, A. 2000. Effect of anoxia on a-amylase induction in maize caryopsis. J. Plant Res., 113:185-92.
41. Güney, D., Bak, Z. D., Aydınoğlu, F., Turna, İ., Ayaz, F. A. 2013. Effect of geographical variation on the sugar composition of the oriental beech (Fagus orientalis Lipsky). Turk J Agric For: 7: 221-230.
42. Hamilton J.G., Zangerl A.R., Delucia E.H., Berenbaum, M.H. 2001. The carbon-nutrient balance hypothesis: Its rise and Fall. Ecology Letters 4: 86-95.
43. Hammond, J.P., White, P. J. 2008. Sucrose transport in the phloem. Integrating root responses to phosphorus starvation. Journal Experimintal Botany 59:109-113.
44. Holdsworth, M., Kurup, S., McKibbin, R. 1999. Molecular and genetic mechanisms regulating the transition from embryo development to germination. Trends Plant Sci. 4, 275±280.
45. Huber, S. C. 1986. Fructose 2,6 biphosphate as a regulatory metabolits in plants. Annu. Rev. Plant. Physiol. Plant Mol Biol. 37:233- 246.
46. Huber, S. C., Huber, J. L. 1996. Role and regulation of sucrose-phosphate synthase in higher plants. Annu. Rev. Plant. Physiol. Plant Mol Biol. 47:431-444.
47. Jang, J. C., Leon, P., Zhou, L., Sheen, J. 1997. Hexokinase as a sugar sensor in higher plants. Plant Cell 9: 5-19.
48. Jang, J. C., Sheen, J. 1997. Sugar sensing in higher plants. Trends Plant Sci 2:208-214.
49. Kaneko, M. Itoh H, Ueguchi-Tanaka M, Ashikari M, Matsuoka M. 2002. The α- amylase induction in Endosperm during Rice Seed Germination Is Caused by Gibberellin Synthesized in Ephithelium. Plant Physiology. 168: 1264-1270.
50. Kimberly, L., Falk, R.H.B., Chengbin Xiang, David J. O. 1998. Metabolic Bypass of the Tricarboxylic Acid Cycle during Lipid Mobilization in Germinating Oilseeds. Plant Physiology, 117, 473-481.
51. Komnrink, E., Kruger, N.J. 1984. Inhibition by metabolic intermediates of pyrophosphate: fructose 6-phosphate phosphotransferase from germinating castor bean endosperm. Z Pflanzenphysiol 114, 443-453.
52. Krugger, R. 1997. Carbohydrate synthesis and degradation. In Plant Metabolism, 2nd ed, DT Dennis, Turpin D.H., Lefebvre D.D., Layzell D.B., Longman, Singapore, pp:83-104.
53. Lebedev, S.I., Baranskil, P.I., Litrimennko, L.G., Shiyan, L.T. 1975. Physiobiochemical characteristics of plants after presowing treatment with a permanent magnetic field. Soviet Plant Physiology. 22. 84–89.
54. Levin, M., Ernst, S. G. 1997. Applied DC magnetic fields cause alterations in the time of cell divisions and developmental abnormalities in early sea urchin embryos. Bioelectromagnetics 18:255–263.
55. Lin, Y. H., Wimer, L. T., Huang, A.H.C. 1983. Lipase in the lipid bodies of corn scutella during seedling growth. Plant Physiol 73: 460- 463.
56. Magnusson, M. 1984. Magnetic treatment of the nutrient solution for tomatoes and the influence of a magnetic field on water and plants. Rapport Ins For Tradgardsvetenskap, Sveriges, Lant Bruksuniversite, 30: 42.
57. Matsuda, T., Asou, H., Kobayashi, M., Yonekura, M. 1993. Influences of magnetic fields on growth and fruit production of strawberry. Acta Hort. 348, 378-380.
58. Minchin, P.E., Lacointe, A. 2005. New understanding of phloem physiolgy ans possible consequens for modelling long-distance carbon transport. New Phytologist 166:771-779.
59. Namba, K. 1996. Effects of alternating magnetic field on plant growth. Sci. Reports Fac. Agric. Okayama Univ. 85, 115-117.
60. Namba, K., Mohri, M., Sasao, S., Shibusawa, S. 1998. Effects of impulse electromagnetic field on plant germination. ASAE Annual International Meeting, Orlando, Florida, USA, 12-16 July.
61. Namba, K., Sasao, A., Shibusama, S. 1995. Effect of magnetic field on germination and plant growth. Acta Hort 399:143–145.
62. Nielsen, T. H., Rung, J.H., Villadsen, D. 2004. Fructose 2,6-bisphosphate: Atraffic signai in plant metabolism. Trends in Plant Science 9:556-563.
63. Nomura, T., Kono, Y., Akazawa, T. 1969. Enzymic mechanism of starch breakdown in germinating rice seeds. II. Scutellum as the site of sucrose synthesis. Plant Physiology 44, 765-769.
64. Novitsky, Y.I., Novitskaya, G.V., Kocheshkova, T.K., Nechiporenko, G.A., Dobrovol Skii, M.V. 2001. Growth of green onions in a weak permanent magnetic field. Russian Juornal of Plant Physiology. 8:709-716.
65. Palmiano, E. P., Juliano, B. O. 1972. Biochemical changes in the rice grain during germination. Plant Physiology 49, 751–756.
66. Pearson, D., Melon, H.K., Ronald, S. 1976. Chemical analysis of Food,8th edition. Churchill Livingstone. Pp 5-63.
67. Phirke, P.S., Kubde, A. B., Umbarkar, S. P. 1996. The influence of magnetic field on plant growth. Seed Sci Technol 24:375-392.
68. Pintilie, M., Oprica, L., Surleac, M., Dragutivan, S., Creanga, D.E. 2006. Enzyme Activity in Plants Treated with magnetic solution. Rom. Journ. Phys., Vol. 51, Nos. 1–2, P. 239-244.
69. Plaxton, W. C. 1996. The organisation and regulation of plant glycolysis. Annu. Rev. Plant Physiol. Plant Mol. Biol. Pp:47:185-214.
70. Podlesny, J., Misiak, L.E., Podlesna, A., Pietruszewski, S. 2005. Concentration of free radicals in pea seeds after pre-sowing treatment wirth magnetic field. International Agrophysics. Vol 19. No.3. pp 243-249.
71. Porch, T. G. 2006. Application of stress indices for heat tolerance screening of common bean. J. Agron. Crop Sci. 192, 390–394.
72. Pourakbar, L. 2013. Effect of Static Magnetic Field on Germination, Growth Characteristics and Activities of Some Enzymes in Chamomile Seeds (Matricaria chamomilla L.). International Journal of Agronomy and Plant Production. Vol., 4 (9), 2335-2340.
73. Pourakbar, L., Hatami, S. 2012. Exposure of Satureia hortensis L. Seeds to Magnetic Fields: Effect on GerminationGrowth characteristic, and activity of Some enzymes. Journal of Stress Physiology & Biochemistry. Vol 8. No. 21: 191-198.
74. Prasad, P. V. V., Boote, K. J., Allen, Jr, L. H., and Thomas, J. M. G. 2002. Effects of elevated temperature and carbon dioxide on seed-set and yield of kidney bean (Phaseolus vulgaris L.). Global Change Biol. 8, 710–721.
75. Prasad, P. V. V., Craufurd, P. Q., and Summerfield, R. J. 2000. Effect of high air and soil temperature on dry matter production, pod yield and yield components of groundnut. Plant Soil 222, 231–239.
76. Price, J., Li, T.C., Kang, S. G., Na, J. K., Jang, J.C. 2003. Mechanisms of glucose signaling during germination of Arabidopsis. Plant Physiol. 132 : 1424 – 1438.
77. Rahamatalla, A.B., Babiker, E.E., Krishna, A.G. 2001. Changes in fatty acids composition during seed growth and physicochemical characteristics of oil extracted from four saffl ower cultivars. Plant Foods Human Nut 56: 385-395, 2001.
78. Rezaei, A., Nasery, B., Yazdian, F., Mhedayatı, Ma. 2011. A study of gibberalic acid effect on oriental beech (Fagus orientalis L.) nuts dormancy removal. In: Wagner S, Fahlvik N, Fischer H (eds) Proceedings of The 9th IUFRO International Beech Symposium organized by IUFRO working party 1.01.07 “Ecology and Silviculture of Beech” Dresden/Göttingen, Germany, 12- 17 September 2011. Institute for Silviculture and Forest Protection, Dresden, Germany, pp 61-63.
79. Rita T. T., Knorpp, C., Glimelius, K. 2005. Modified sucrose, starch, and ATP levels in two alloplasmic male-sterile lines of B. napus. Journal of Experimental Botany, 56(414), 1245–1253.
80. Rochalska M, Orzeszko-Rywka, A. 2005. Magnetic field treatment improves seed performance. Seed Sci Technol 33:669-674.
81. Rolland, F., Baena-Gonzalez, E., Sheen, J. 2006. Sugar sensing and signaling in plants: Conserved and novel mechanisms. Annu Rev Plant Biol 57:675-709.
82. Roper, T.R., J.D. Keller, W.H. Loescher, Rom, C.R. 1988. Photosynthesis and carbohydrate partitioning in sweet cherry: Fruiting effects. Physiol. Plant. 72:42-47.
83. Saatçioğlu, F. 1969. Silvikültürün Biyolojik Esasları ve Prensipleri. İ.Ü Orman Fakültesi, İ.Ü Yayın No: 1429, O.F Yayın No: 138, İstanbul, 323 s.
84. Samy, C.G. 1998. Magnetic seed treatment. I. Influence on flowering, siliquae and seed characteristics of cauliflower. Orissa J. Hort. 26, 68-69.
85. Schulz, A. 1994. Phloem transport and differential unloading in pea seedlings fluorescense microscopy. Protoplasma, 166:153-164.
86. Stange, B. C., Rowland, R. E,, Rapley, B. I., Podd, J. V. 2002. ELF magnetic fields increase amino acid uptake into Vicia faba L. roots and alter ion movement across the plasma membrane. Bioelectromagnetics 23:347–354.
87. Stitt, M., Gibon, Y., Lunn, J. E., Piques, M. 2007. Multilevel genomics analysis of carbon signalling during low carbon availability: Coordinating the supply and utilisation of carbon in a fluctuating environment. Funct Plant Biol 34:526-549.
88. Subbarao, K.V., Datta, R. and Sharma, R. 1988. Amylases synthesis in scutellum and aleurone layer of maize seeds. Phytochemistry, 49:657- 666.
89. To, J.P., Reiter, W.D., Gibson, S. I. 2002. Mobilization of seed storage lipid by Arabidopsis seedlings is retarded in the presence of exogenous sugars. BMC Plant Biol 2: 4.
90. Torres, S, Porras Leon, E., Fernandez C., R. 1999. Effects of magnetic treatment of tomato (Lycopersicon esculentum Mill) seeds on germination and seedling growth. Hort. Abst. 70, 68-92.
91. Vashisth, A., Nagarajan; S. 2010. Characterization of Water distribution an Activities of Enzymes during Germination in Magnetically-exposed Maize (Zea mays L.) Seeds. Indian Journal of Biochemistry and Biophysics. Vol 47. pp. 311-38.
92. Winter, H., Huber, S. C. 2000. Regulation of Sucrose Metbolism in Higher Plants: Localisation and Regulation of Activity of Key Enzymes. Critical Review Plants Sciences 19:31-67.
93. Xu, F., Tan, X., Wang, Z. 2010. Effects of Sucrose on Germination and Seedling Developmentof Brassica Napus. International Journal of Biology. 2(1).
94. Yılmaz, M. 2005. The researches on the physiology of oriental beech (Fagus orientalis Lipsky.) seeds. PhD thesis, Istanbul University, Faculty of Forestry, Istanbul, Turkey, 170 p.
95. Zeaaman, S.C., Smith, S.M., Smith, A.M. 2007. The diurnal metabolism of leaf starch. Biochemical Journal 401:13-28.
96. Zimmermann, M. H., Ziegler, H. 1975. List of sugars and sugar alcohols in sieve-tube exudates. In (Zimmermann MH., Milburn., JA (eds) Encycplopedia of plant physiology, Springer Verlag. Hridelberg, Vol, 1, 480-503.