A contribution to Onobrychis sect. Hymenobrychis Fabaceae in East of Iran

Abstract

Chromosome number, meiotic behavior and morphological characters related to habit and pollen grains were studied in Onobrychis bojnurdensis Ranjbar & Hajmoradi as a new species belonging to Onobrychis sect. Hymenobrychis from East of Iran. The new species is related to O. sintenisii but differs from it in a few morphological characters such as plant indumentums, size of stipule, bract and different part of the flower. We evaluated and determined the species limits within Eastern species of O. sect. Hymenobrychis, employing multivariate statistics. Results obtained from morphological and micromorphological studies confirm novelty of O. bojnurdensis. Cytogenetic study indicates that the O. bojnurdensis is a diploid plant and possesses 2n = 2x = 14 chromosome number. The general meiotic behavior was regular, with bivalent pairing and normal chromosome segregation at meiosis. This report is the first cytogenetic analysis of the new taxon

Keywords

• Fabaceae,Iran,meiosis,new species,Onobrychis sect. Hymenobrychis

References

1. Baltisberger, M. 1991. IOPB chromosome data 3. IOPB Newsletter. 17: 5-7.
2. Boissier, E. 1872. Onobrychis. In (Ed) Boissier, E. Flora Orientalis. Geneva, Basilease and Lugundi. pp. 545-553.
3. Erdtman, G. 1960. The acetolysis method. Svensk Botanisk Tidskrift. 54: 561-564.
4. Faegri, K. 1956. Palynological studies in NW European Papilionaaceae. Botany Museum Bergen.
5. Faegri, K., Iversen, J. 1989. Textbook of pollen analysis. Blackwell Science Publishing, Chichester.
6. Ghaffari, S. M. 2006. Occurrence of diploid and polyploidy microspores in Sorghum bicolor (Poaceae) is the result of cytomixis. African J. Biotech. 5: 1450-1453.
7. Hedge, I. C. 1970. Onobrychis. In: (Ed) Davis, P. H., Flora Turkey and the Aegean Islands. Edinburgh: Edinburgh University Press. pp: 584-589.
8. Hesamzadeh Hejazi, S. M., Ziaei Nasab, M. 2010. Cytotaxonomy of some Onobrychis (Fabaceae) species and populations in Iran. Caryologia. 63: 18-31.

9. Karamian, R., Moradi Behjou, A., Ranjbar, M. 2009. Pollen morphology of the Onobrychis sect. Heliobrychis in Iran. T. B. J. 1: 63- 72.
10. Karshibaev, H. K. 1992. Chromosome numbers of some Fabaceae in Uzbekistan. Tezisy 3 Soveshchanie Po Kariologii Rastenii. 27: 1-2.
11. Kovach, W. 1985-2002. Institute of Earth Studies, University College of Wales, Aberystwyth, (Shareware), MVSP Version 3.2, Kovach Computing Services http://www.kovcomp.com/MVPs/downl2.html.
12. Khatoon, S., Ali, S. 1991. Chromosome numbers in subfamily Papilionoideae (Leguminosae) from Pakistan. Willdenowia. 20: 159- 165.
13. Jones, N., Houben, A. 2003. B-chromosomes in plant: escapees from the A-chromosome genome? T. P. S. 8: 417-423.
14. Lock, J. M., Simpson, K. 1991. Legumes of West Asia, a check-list. Royal Bot. Gardens, Kew.
15. Mabberley, D. J., 1997. The plant book. A portable dictionary of the vascular plants, 2nd ed. Cambridge University.
16. Melhem, T. 1971. Pollen grains of plants of the Cerrado- Leguminosae- Lotoideae Tribe Phaseoleae. Hoehnea 1: 119-151.
17. Mesicek, J., Sojak, J. 1992. Chromosome numbers of Mongolia angiosperms. Preslia. 64: 193-206.
18. Moore, P. D., Webb, J. E., Collinson, M. E. 1991. Pollen analysis (2 Ed.). Blackwell scientific publications, Oxford.
19. Nimala, A., Rao, P. N. 1996. Genetics of chromosome numerical mosaism in higher plants. The nucleus 39: 151-175.
20. Ohashi, H. 1971. A taxonomic study of the tribe Coronilleae (Leguminosae) with special reference to pollen morphology. Journal of the Faculty of Science, University of Tokyo 11: 25-92.
21. Pagliarini, M. S. 2000. Meiotic behavior of economically important plant species: the relationship between fertility and male sterility. Genet. Mol. Biol. 23: 997-1002.
22. Pavlova, D. K., Manova, V. I. 2000. Pollen morphology of the genera Onobrychis and Hedysarum (Hedysareae, Fabaceae) in Bulgaria. Ann. Bot. Fen. 37: 207-217.
23. Pire, S. 1974. Studio palynologico de la tribu Hedysareae (Leguminosae). Bonplandia. 3(12): 143-168.
24. Ranjbar, M. 2009. Onobrychis oshnaviyehensis sp. nov. (sect. Hymenobrychis, Fabaceae) from Iran. Nordic J. Bot. 27: 1-5.
25. Ranjbar, M., Amirabadizadeh, H., Karamian, R., Ghahremani, M. A. 2004 Notes on Onobrychis sect. Heliobrychis (Fabaceae) in Iran. Willdenowia. 34: 187-190.
26. Ranjbar, M., Hajmoradi, F., Karamian, R. 2010a. Mitotic study of some species of Onobrychis sect. Hymenobrychis DC. in Iran. I. J. P. B. 1: 47-54.
27. Ranjbar, M., Hajmoradi, F., Karamian, R. 2011. Meiotic chromosome number and behaviour of Onobrychis alborzensis (Fabaceae): a new species from northern Iran. Feddes Repert. 122: 1-12.
28. Ranjbar, M., Hajmoradi, F., Karamian, R. 2012 An overview on cytogenetics of the genus Onobrychis (Fabaceae) with special reference to O. sect. Hymenobrychis from Iran. Caryologia. 65 (3): 187-198.
29. Ranjbar, M., Karamian, R., Afsari, S. 2010b. Meiotic chromosome number and behaviour of Onobrychis avajensis (Fabaceae): a new species from western Iran. Plant Ecol. Evol. 143: 170-175.
30. Ranjbar, M., Karamian, R., Hadadi, A. 2009a. Biosystematic study of Onobrychis vicifolia Scop. and Onobrychis altissima Grossh. (Fabaceae) in Iran. I. J. B. 15: 85-95.
31. Ranjbar, M., Karamian, R., Hadadi, A. 2010c. Cytosystematics of three Onobrychis species (Fabaceae) in Iran. Caryologia. 63: 237- 249.
32. Ranjbar, M., Karamian, R., Hajmoradi, F. 2009b. Taxonomic notes on Onobrychis sect. Hymenobrychis (Fabaceae, Hedysareae) in Iran. Novon. 19: 215-218.
33. Ranjbar, M., Karamian, R., Hajmoradi, F. 2010d. Chromosome number and meiotic behavior of two populations of Onobrychis chorassanica Bunge sect. Hymenobrychis in Iran. J. Cell Mol. Res. 2: 49-55.
34. Ranjbar, M., Karamian, R., Tolui, Z., Amirabadizadeh, H. 2007. Onobrychis assadii (Fabaceae), a new species from Iran. Ann. Bot. Fen. 44: 481-484.
35. Ranjbar, M., Karamian, R., Vitek, E. 2010e. Onobrychis bakuensis (Fabaceae), a new species from Azerbaijan Ann. Bot. Fen. 47: 233-236.
36. Ranjbar, M., Karamian, R., Vitek, E. 2010f. Notes on Onobrychis sect. Hymenobrychis (Fabaceae) in Tajikistan, with the description of a new species. Nord. J. Bot. 28: 1-4.
37. Rechinger, K. H. 1984. Onobrychis. In (Ed) Rechinger, K. H. Flora Iranica. Akademische Druck- und Verlagsanstalt, Graz. 157. pp: 389-459.
38. Spss. 1999. Statistical software package of the social sciences vol. 10.0. spss Inc., Chicago, Illinois.
39. Yakovlev, G. P., Sytin, A. K., Roskov, Y. R. 1996. Legumes of Northern Eurasia, a check-list. Royal Bot. Gardens, Kew.