Hypoglycemic activity of a dietary mushroom Pleurotus florida on alloxan induced diabetic rats

Abstract

Species of Pleurotus possess various medicinal properties including hypoglycemic and hypocholesterolemic activities. The feeding effect of the mushroom Pleurotus florida in powdered form was evaluated on growth, blood glucose and cholesterol level in alloxan-induced diabetic albino rats against control. The starch was estimated in dry mushroom and found to be 0.28% dry weight . The result indicated no significant difference in respect of gaining body weights between Bengal gram-fed and mushroom-fed groups. For studying antidiabetic effect, the control group and half of alloxan-induced 100 mg/kg i.p experimental groups were fed on normal diet with Bengal gram whereas the other half of alloxan-induced group was fed on same diet but with additional mushroom powder. Blood samples were analysed for glucose, serum cholesterol, triglycerides and uric acid levels on 7th, 14th, 21st and 28th days of alloxan treatment. Body weights and mortality were noted during the period of study. The diabetic group administered with mushroom diet showed significantly decreased level of blood glucose and cholesterol compared to the counterpart. Estimation of lipid profile revealed much lower LDL cholesterol in mushroom fed diabetic group than in nonmushroom fed diabetic control group. The study, thus, points out the prominent effect of feeding dried mushroom P. florida on growth of albino rat and positive effect of P. florida on lowering blood glucose and cholesterol level in diabetic rats thereby suggesting its hypoglycemic and hypocholesterolemic effect

Keywords

• Alloxan induced diabetic,Blood glucose,LDL cholesterol,Pleurotus florida,Total cholesterol,Triglycerides

References

1. Alberti, K.G.M.M., Zimmet, P., De Fronzo, R.A. 1997. International Textbook of Diabetes Mellitus. Second ed., John Wiley and Sons, Chichester, UK.
2. Badole, S.L., Bodhankar, S.L. 2007. Interaction study of aquous extract of Pleurotus pulmonarius (Fr.) Quel.-Champ with acarbose in alloxan induced diabetic mice. J. Appl. Biomed. 5: 157-166.
3. Bandopadhyay, S., Chatterjee, N.C. 2009. Water hyacinth, a low cost supplement to cultivation of Pleurotus florida. Mushroom Res. 18: 5-9.
4. Bobek, P., Ozdin, L., Galbavy, S. 1998. Dose and time-dependent hypocholesterolemic effect of oyster mushroom (Pleurotus ostreatus) in rats. Nutrition. 14: 282-286.
5. Croan, S.C. 2004. Conversion of conifer wastes into edible and medicinal mushroom. Forest Prod. J. 54: 68-76.
6. Francia, C., Rapior, S., Courtecuisse, R., Siroux, Y. 1999. Current research findings on the effects of selected mushrooms on cardiovascular diseases. Int. J. Med. Mushrooms. 1: 169-172.
7. Gray, A.M., Flatt, P.R. 1998. Insulin-releasing and insulin-like activity of Agaricus campestris (mushroom). J. Endocrinol. 157: 259-266.
8. Gumieniczek, A., Hopkala, H., Rolinski, J., Bojarska-Junak, A. 2005. Antioxidative and anti inflammatory effects of repaglinide in plasma of diabetic animals. Pharmacol. Res. 52: 162-166.

9. Gunde-Cimerman, N. 1999. Medicinal value of the genus Pleurotus (Fr.) P. Karst. (Agaricales S.I. Basidiomycetes). Int. J. Med. Mushrooms. 1: 69-80.
10. Halliwell, B., Gutteridge, J.M. 1990. Role of free radicals and catalytic metal ions in human disease; an overview. Methods in Enzymol. 186: 1-85.
11. Hobbs, C. 1995. Medicinal Mushrooms: An Exploration of Tradition, Healing and Culture, Botanica Press, Santa Cruz, CA., USA.
12. Hwang, H.J., Kim, S.W., Lim, J.M., Joo, J.H., Kim, H.O., Kim, H.M., Yun, J.W. 2005. Hypoglycemic effect of crude exopolysaccharides produced by a medicinal mushroom Phellinus baumii in streptozotocin-induced diabetic rats. Life Sci. 76: 3069-3080.
13. Jain, S.K., Kannan, K., Lim, G. 1998. Ketosis (acetoacetate) can generate oxygen radicals and cause increased lipid peroxidation and growth inhibition in human endothelial cells. Free Rad. Biol Med. 25: 1083-1088.
14. Kiho, T., Morimoto, H., Sakushima, M., Usui, S., Ukai, S. 1995. Polysaccharide in fungi. XXXV.1) Antidiabetic activity of an acidic polysaccharide from the fruiting bodies of Tremella aurantia. Biol. Pharmaceut. Bull. 18: 1627-1629.
15. Kiho, T., Sobue, S., Ukai, S. 1994. Structural features and hypoglycemic activities of two polysaccharides from a hot- water extract of Agrocybe cylindracea. Carbohydr. Res. 251: 81-87.
16. Kiho, T., Yamane, A., Hui, J., Usui, S., Ukai, S. 1996. Polysaccharide in fungi, XXXVI.1) Hypoglycemic activity of a polysaccharide (CS-F30) from the cultural mycelium Cordyceps sinensis and its effect on glucose metabolism in mouse liver. Biol. Pharmaceut. Bull. 19: 294-296.
17. Konno, S., Tortorelis, D.G., Fullerton, S.A., Samadi, A.A., Hettiarachchi, J., Tazaki, H.A. 2001. Possible hypoglycaemic effect of maitake mushroom on type 2 diabetic patients. Diabetic Med. 18: 1010.
18. Lo, H.C., Tu, S.T., Lin, K.C., Lin, S.C. 2004. The anti-hyperglycemic activity of the fruiting body of Cordyceps in diabetic rats induced by nicotinamide and streptozotocin. Life Sci. 74: 2897-2908.
19. Lue, G., Fruchart, J.C. 1991. Oxidation of lipoproteins and atherosclerosis. Am. J. Clinical Nutr. 53: 2065-2095.
20. Nicasio, P., Aguilar-Santamaria, L., Aranda, E., Ortiz, S., Gonzalez, M. 2005. Hypoglycemic effect and chlorogenic acid content in two Cecropia species. Phytother Res. 19: 661-664.
21. Packer, L., Rfsen, P., Tritschler, H.J., King, G.L., Azzi, A. 2000. Antioxidants in Diabetes Management. Marcel Dekker, New York.
22. Roeschlau, P., Bernt, E., Gruber, W. 1974. Enzymatic determination of total cholesterol in serum. Zeitschrift Klin. Chemie Klin. Biochem. 12: 226.
23. Sadasivam, S., Manickam, A. 1996. Biochemical Method. Second ed., New Age International (P) Limited, Publishers and Tamil Nadu Agricultural University, Coimbatore, India.
24. Swanston-Flatt, S.K., Day, C., Flatt, P.R., Gould, B.J., Bailey, C.J. 1989. Glycaemic effects of traditional European plant treatments for diabetes. Studies in normal and streptozotocin diabetic mice. Diabetes Res. 10: 69-73.
25. Talpur, N., Echard, B., Dadgar, A., Aggarwal, S., Zhuang, C., Bagchi, D. 2002. Effects of Maitake mushroom fractions on blood pressure of zucker fatty rats. Res. Comm. Mol. Path. Pharmacol. 112: 68-82.
26. Yang, B.K., Kim, D.H., Jeong, S.C., Das, S., Choi, Y.S., Shin, J.S., Lee, S.C., Song, C.H. 2002. Hypoglycemic effect of a Lentinus edodes exo-polymer produced from a submerged mycelial culture. Biosci. Biotechnol. Biochem. 66: 937-942.
27. Yuan, Z., He, P., Cui, J., Takeuchi, H. 1998. Hypoglycemic effect of water-soluble polysaccharide from Auricularia auricular-judae Quel. on genetically diabetic KK-Ay mice. Biosci. Biotechnol. Biochem. 62: 1898-1903.