Relationships among some endemic Hieracium L. s.str. Asteraceae taxa based on internal transcribed spacer ITS

Abstract

In the current study, thirteen individuals from ten endemic Hieracium Asteraceae taxa were analyzed by using sequences of the internal transcribed spacers ITS of nuclear ribosomal DNA in order to see whether the ITS could provide additional useful information for the relationships of the genus or not. Therefore, total genomic DNA was isolated from healthy leaves of each individual collected from different localities of North-east NE Anatolia Turkey . Amplification of the entire ITS region was performed by using universal primers with the aid of the polymerase chain reaction PCR . Neighbor Joining NJ and Maximum Parsimony MP trees were constructed based on the ITS sequences to elucidate the phylogenetic relationships among the investigated taxa. The aligned sequences comprised 710 characters. Divergence values among the individuals ranged from 0.0 to 2.1%. The ITS data provided sufficient variation to resolve interspecific relationships, but the topologies of the NJ and MP trees did not support conventional subgeneric classification of the species

Keywords

• Asteraceae,Hieracium,ITS,nrDNA,Turkey

Türkiye’ de yayılış gösteren bazı endemik Hieracium Asteraceae taksonlarının ITS dizilerine dayalı akrabalıkları

Abstract

Bu çalışmada, 10 endemik Hieracium L. taksonuna ait 13 farklı birey, nüklear ribozomal DNA ITS bölgesinin cinsin akarabalık ilişkilerini ortaya koyabilecek yeni bilgiler sağlayıp sağlayamayacağını belirlemek amacıyla karşılaştırılmıştır. Bunun için öncelikle Kuzey Doğu Anadolu Bölgesi’nin değişik yerlerinden toplanan bireylerin sağlıklı yapraklarından genomik DNA’ları elde edilmiştir. Evrensel oligonükleotidler kullanılarak bireylere ait ITS bölgeleri polimeraz zincir reaksiyonu ile çoğaltılmıştır. Taksonlar arasındaki filogenetik ilişkiyi ortaya çıkarmak için Neighbour-Joining NJ ve Maksimum Parsimoni MP ağaçları çizilmiştir. Baz dizinlerinin hizalanması sonucu 710 karakterden oluşan bir veri seti elde edilmiş ve örnekler arasındaki divergens değerlerinin % 0.0-2.1 arasında değiştiği bulunmuştur. ITS bölgesi taksonlar arasındaki ilişkiyi açıklama da yeterli bilgi sağlamasına rağmen analiz sonucu elde edilen NJ ve MP ağaçlarının topolojileri, cinsin seri seviyesinde geleneksel taksonomik verileriyle uyum göstermemiştir

Keywords

• Asteraceae,Hieracium,ITS,nrDNA,Türkiye

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