Ultrastructure of mature pollen grains in diploid and the natural tetraploid Trıfolium pratense

Abstract

Within the scope of this study, the ultrastructural changes in diploid and natural tetraploid Trifolium pratense L. Elçi red clover were examined from tetrad stage to mature pollen stage. In both diploid and natural tetraploid types, it was identified that the mature pollens were two-celled, the generative cell was oval-shaped and situated adjacent to pollen wall and the vegetative cell was elipsoidal-shaped. In both types, the vegetative cell included abundant vesicles, ribosomes and mitochondria.. Agranular ER, plastids and dictyosomes were low in number. In the tetraploid type, abundant ER foldings parallel to the cell wall were remarkable around the generative cell. The generative cell, containing a low quantity of mitochondria, ribosomes and vesicles, had a large nucleus at the center. It is noteworthy that in both types, there were great quantities of electron-dense vesicles

Keywords

• mature pollen grains,Trıfolıum pratense.,ultrastructure

Diploid ve doğal tetraploid Trifolium pratense’de olgun polenin ince yapısı

Abstract

Bu çalışmada, diploid ve doğal tetraploid Trıfolıum pratense L. Elçi çayırüçgülü ’ de tetrad evresinden olgun polen evresine kadar polenlerdeki ultrastrüktürel değişmeler incelenmiştir.Hem diploid hem de tetraploid örneklerde olgun polenlerin iki hücreli olduğu, generatif hücrenin oval şekilli ve polen çeperine yakın yer aldığı, vejetatif hücrenin ise elipsoidal şekilli olduğu gözlendi. Her iki tipde de vejetatif hücrede çok sayıda küçük vesiküller, ribozom ve mitokondri mevcuttur. Granülsüz endoplazmik retikulum ER , plastidler ve diktiyozomlar az sayıdadır. Tetraploid polenlerde generatif hücre çevresinde bol miktarda hücre membranına paralel ER katlanmaları dikkati çekmiştir. Az sayıda mitokondri, ribozom ve vesikül içeren generatif hücrenin merkezi olarak yer alan büyük bir çekirdeğinin bulunduğu gözlenmiştir. Her iki tipde de elektronca yoğun çok miktarda vesiküllerin bulunması dikkati çekmiştir

Keywords

• olgun polen,Trıfolıum pratense.,ince yapı

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