Time dependent cytotoxic role of Homalothecium sericeum extracts on glioma

Abstract

Bryophytes have been used as medicinal plants for more than 400 years in China, Europe and North America. There is also evidence confirming the antibiotic and anticancer activity of Bryophytes against, prokaryotes, fungi and different cancer cells. The purpose of the current study was to investigate cytotoxic property of Homalothecium sericeum hedw. schimp., which is a bryophyte, extracts on rat glioma C6 cells for 48 hrs, in vitro. We first collected two different acetone and A extracts from H. sericeum by two different extraction processes. C6 cells were seeded in 96 well plates 2x104 cells/well and incubated for 24 hrs. Following this incubation period, the medium was replaced with only medium control or medium with extracts at concentrations of 0.17, 1.7, 17, 85 or 170 µg/mL for 48 hrs. Cytotoxicity was determined by using 3- 4,5-dimethylthiazol-2yl - 2,5-diphenyltetrazolium bromide MTT colorimetric assay. Acetone extract of H. sericeum at 0.17, 1.7 and 17 µg/mL concentrations did not change the survival rate of C6, but 85 and 170 µg/mL inhibited about 16 % and 36 % after 48 hr p

Keywords

• Homalothecium sericeum,Bryophyta,Glioma,MTT,Cytotoxicity

Homalothecium sericeum özütlerinin zamana bağlı olarak glioma hücrelerinin çoğalması üzerindeki etkileri

Abstract

Bryofitler 400 yıldan fazla bir süredir Çin, Avrupa ve Kuzey Amerika’da bitkisel ilaç olarak kullanılmaktadır. Bryofitlerin prokaryot, mantar türleri üzerinde antibiyotik ve farklı kanser hücreleri üzerinde antikanser etkinlik gösterdiği konusunda veriler bulunmaktadır. Bu çalışmanın amacı, bir Bryofit olan Homalothecium sericeum’un sıçan glioma C6 hücreleri üzerinde 48 saatlik sitotoksik etkinliğini araştırmaktır. İlk olarak, iki farklı özüt elde etme yöntemiyle aseton ve A özütlerini elde ettik. C6 hücreleri 96 kuyucuklu kültür kaplarına ekilerek 2x104 hücre/kuyucuk 24 saat inkübe edildi. İnkübasyon süresinin ardından kuyucuklara özütlerin 0.17, 1.7, 17, 85 ve 170 µg/mL konsantrasyonları eklenerek 48 saat muamele edildi. Sitotoksisite, MTT [3- 4,5-dimetiltiazol-2yl - 2,5difeniltetrazolyum bromid] yöntemi ile belirlendi. 48 saat sonunda aseton özütünün 0.17, 1.7 ve 17 µg/mL konsantrasyonları C6 hücre canlılığını değiştirmezken, 85 ve 170 µg/mL konsantrasyonları hücre canlılığını sırası ile %16 ve %36 oranında azalttı p

Keywords

• Homalothecium sericeum,Bryofit,Glioma,MTT,Sitotoksisite

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