Diversity of soil fungi exposed to fresh and stored Olive Mill Wastewater

Year 2010, Volume: 3 Issue: 1, 1 - 9, 15.04.2010

Abdelali Fadıle Mohamed Merzoukı Mohamed Benlemlıh

Abstract

Olive Mill Wastewater OMW management is one of the most challenging environmental problems in Mediterranean countries. Its recycling in soil is an alternative of valorization mean of interest. The effect of OMW on growth of soil fungi, a principal element of biodegradability in soil, was investigated in this study. In a field trial, OMW application to soil at 8 l/m² and 16 l/m² caused an increase in abundances of soil fungi during the 6 months following the spreading. In a microcosm essay, growth of soil fungi was better in fresh OMW than in stored OMW becoming after storage more antimicrobial and phytotoxic. In fresh OMW sterilized then inoculated by soil microflora, survivors of soil fungi were constituted mainly by yeasts which showed an increase of abundances from 5.09 104 CFU/ml to 5.02 108CFU/ml after 15 days of incubation at 20°C. In stored sterilized then inoculated OMW, yeasts showed a fast reduction then a survival at low levels. Soil moulds were a sensitize group to OMW even fresh or stored. This group presented a fast reduction of abundances then disappearance. It could be concluded that OMW spreading in high amounts would conflict soil fungi’s homeostasis and that spreading of OMW which was stored for a prolonged period should be avoided

Keywords

Olive Mill Wastewater , fungi , soil , Olive Mill Wastewater storage

References

• Ben Sassi, A., Ouazzani, N., Walker, G., Ibnsouda, S., El Mzibri, M., Boussaid, A. 2007. Detoxification of olive mill wastewaters by Moroccan yeast isolates. Biodegradation. 19. 337-346.
• Borja, R., Martin, A., Alonso, V., Garcian I., Banks, C.J. 1995. Influence of different aerobic pre-treatment on the kinetics of anaerobic digestion of olive mill wastewater. Water Research. 19. 489-495.
• Boukhoubza, F., Ait Boughrous, A., Yacoubi-Khebiza, M., Jail, A., Hassani, L., Loukili Idrissi, L., Nejmeddine, A. 2008. Impact of olive oil wastewater on the physicochemical and biological quality of groundwater in the Haouz plain, south of Marrakesh (Morocco). Environmental Technology. 29. 959-974.
• Box, J.D. 1983. Investigation of the Folin-Ciocalteau phenol reagent for the determination of polyphenolic substances in natural waters. Water Research. 17. 511-522.
• Brandi, G., Sisti, M., Schiavano, G., Salvaggio, L., Albano, A. 1996. Survival of Aeromonas hydrophila, Aeromonas caviae and Aeromonas sobrio in soil. Journal of Applied Bacteriology. 81. 439-444.
• Cabrera, F., Lopez, R., Martinez-Bordiu, A., Dupuy de Lome, E., Murillo, J.M. 1996. Land treatment of olive mill waste water. International Biodeterioration and Biodegradation. 38. 215-225.
• Capasso, R., Evidente, A., Schivo, L., Orru, G., Marciallis, M.A., Cristinzio, G. 1995. Antibacterial polyphenols from olive mill waste waters. Journal of Applied. Bacteriology. 79. 393-398.
• Casa, R., D’Annibale, A., Pieruccetti, F., Stazi, S.R., Giovannozzi Sermanni, G. G., Lo Cascio, B. 2003. Reduction of the phenolic components in olive-mill wastewater by enzymatic treatment and its impact on durum wheat (Triticum durum Desf.) germinability. Chemosphere. 50. 959-966.
• D’Annibale, A., Casa, R., Pieruccetti, F., Ricci, M., Marabottini, R. 2004. Lentinula edodes removes phenols from olive mill waste water: impact on durum wheat (Triticum durum Desf.) germinability. Chemosphere. 54. 887-894.
• Davies, C., Long, J., Donald, M., Ashbolt, N. 1995. Survival of fecal microorganisms in marine and fresh water sediments. Applied and Environmental Microbiology. 61. 1888-1896.
• Di Giovacchino, L., Basti, C., Costantini, M.L., Ferrante, M.L., Surrichio, G. 2001. Effects of olive mill waste water spreading on soil cultivated with maize and grapevine. Agricultura Mediterranea. 131. 33-41.
• El Hajjouji, H., Ait Baddi, G., Yaacoubi, A., Hamdi, H., Winterton, P., Revel, J.C., Hafidi, M. 2008. Optimisation of biodegradation conditions for the treatment of olive mill wastewater. Bioressource Technology. 99. 5505-5510.
• Evelyn, H., Michael, P., Christina, D., Gert, B., Sophie, Z.B. 2005. Composition of the microbial communities in the mineral soil under different types of natural forest. Soil Biology and Biochemistry. 37. 661-671.
• Field, J.A., Lettinga, G. 1989. The effect of oxidative coloration on the methanogenic toxicity and anaerobic biodegradability of phenols. Biological Wastes. 29. 161-179.
• Gamba, C., Piovanelli, C., Papini, R., Pezzarossa, B., Ceccarini, L., Bonari, E. 2005. Soil microbial characteristics and mineral nitrogen availability as affected by olive oil waste water applied to a cultivated soil. Communications in Soil Science and Plant Analysis. 36. 937-950.
• Inamori. Y., Murakami, K., Sudo, R., Kurihara, Y., Tanaka, N. 1992. Environmental assessment method for field release of genetically engineered microorganisms using microcosm systems. Water Science and Technology. 26. 2161-2164.
• Jarboui, R., Sellami, F., Kharroubi, A., Gharsallah, N., Ammar, E. 2008. Olive mill wastewater stabilization in open-air ponds: Impact on clay-sandy soil. Bioresource Technology. 99. 7699-7708.
• Kim, J. K., Shoda, M. 1999. Purification and characterisation of a novel peroxidase from Geotrichum candidum Dec 1 involved in decolorization of dyes. Applied and Environmental Microbiology. 65. 1029-1035.
• Kotsou, M., Mari, I., Lasaridi, K., Chatzipavlidis, I., Balis, C., Kyriacou, A. 2004. The effect of olive oil mill wastewater (OMW) on soil microbial communities and suppressiveness against Rhizoctonia solani. Applied Soil Ecology. 26. 113-121.
• Lanciotti, R., Gianotti, A., Baldi, D., Angrisani, R., Suzzi, G., Mastrocola, D., Guerzoni, M.E. 2004. Use of Yarrowia lipolytica strains for the treatment of olive mill wastewater. Bioresource Technology. 96. 317-322.
• Marrara, G., Tamburino, V., Zimbone, S.M. 2002. Storage and land application of olive mill wastewater: experiences in Calabria. In: Proceedings of the ASAE Annual International Meeting/CIGR XVth World Congress, July 28– 31, Chicago, Illinois, USA.
• Maunoir, S., Philip, H., Ambaud, A. 1990. Stimulation of psychrophilic methanation with a septic tank biological activator. Water Research. 24. 195-205.
• Mechri, B., Echbili, A., Issaoui, M., Braham, M., Ben Elhadj, S., Hammamia, M. 2007. Short-term effects in soil microbial community following agronomic application of olive mill wastewaters in a field of olive trees. Applied Soil Ecology. 36. 216-223.
• Mekki, A., Dhouib, A., Sayadi, S. 2006. Changes in microbial and soil properties following amendment with treated and untreated olive mill wastewater. Microbiological Research. 161. 93-101.
• Paraskeva, P., Diamadopoulos, E. 2006. Technologies for olive mill wastewater (OMW) treatment: a review. Journal of Chemical Technology and Biotechnology. 81. 1475-1485.
• Ramos-cormenzana, A., Juarez-Jimenez, B., Garcia-pareja, M.P. 1997. Antimicrobial activity of olive mill waste waters (Alpechin) and biotransformed olive mill waste water. International Biodeterioration and biodegradation. 38. 283- 290.
• Rinaldi, M., Rana, G., Introna, M. 2003. Olive mill wastewater spreading in southern italy: effects on a durum wheat crop. Field Crops Research. 84. 319-326.
• Rodier, J. 1996. L’analyse de l’eau, 8éme édition. Dunod, Paris, France.
• Rubia, T.D.L., Lucas, M., Martinez, J. 2008. Controversial role of fungal laccases in decreasing the antibacterial effect of olive mill waste-waters. Bioresource Technology. 99. 1018-1025.
• Saadi, I., Laor, Y., Raviv, M., Medina, S. 2007. Land spreading of olive mill wastewater: Effects on soil microbial activity and potential phytotoxicity. Chemosphere. 66. 75-83.
• Sierra, J., Marti, E., Garau, M., Gruanas, R. 2007. Effects of the agronomic use of olive mill wastewater: Field experiment. Science of the Total Environment. 378. 90-94.
• Tardioli, S., Ba`nne`, E.T.G., Santori, F. 1997. Species-specific selection on soil fungal population after olive mill waste-water treatment. Chemosphere. 34. 2329-2336.
• Tomati, U., Galli, E. 1992. The fertilising value of waste waters from the olive processing industry. Humus, its structure and role in agriculture and environment. J. Kubat ed. Elsevier, pp. 117-126.
• Tomati, U., Zito, V., Sandovar, A., Gaton, P., Ruiz, M. 2001. An European regulation about olive mill waste industry. In: Proceedings of 11th International Symposium on Environmental Pollution and its Impact in the Mediterranean Region, Cyprus, October 6–10, C5, pp. 184.
• Vavilin, V.A., Lokshina, L.Y., Ritov, S.V., Kotsyurbenko, O.R., Nozhevnikova, A.N. 2000. Description of two-steps kinetics in methane formation during psychrophilic H2/CO2 and mesophilic glucose conversion. Bioresource Technology. 71. 195-209.